Strain Fitness in Escherichia coli ECRC62 around BNILDI_08170

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotidesthrC and yaaX are separated by 213 nucleotidesyaaX and BNILDI_08180 are separated by 259 nucleotides BNILDI_08160: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 1,627,791 to 1,630,253 thrA BNILDI_08165: thrB - homoserine kinase, at 1,630,255 to 1,631,187 thrB BNILDI_08170: thrC - threonine synthase, at 1,631,188 to 1,632,474 thrC BNILDI_08175: yaaX - Uncharacterized protein YaaX, at 1,632,688 to 1,632,984 yaaX BNILDI_08180: BNILDI_08180 - IS110-like element IS621 family transposase, at 1,633,244 to 1,634,224 _08180 Position (kb) 1631 1632 1633Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1630.193 kb on + strandat 1630.222 kb on + strandat 1630.222 kb on + strandat 1630.223 kb on - strandat 1630.226 kb on + strandat 1630.226 kb on + strandat 1630.244 kb on - strandat 1630.260 kb on + strandat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.443 kb on + strand, within thrBat 1630.444 kb on - strand, within thrBat 1630.522 kb on - strand, within thrBat 1630.542 kb on - strand, within thrBat 1630.821 kb on + strand, within thrBat 1630.897 kb on + strand, within thrBat 1630.941 kb on + strand, within thrBat 1630.954 kb on + strand, within thrBat 1631.061 kb on - strand, within thrBat 1631.114 kb on - strandat 1631.143 kb on - strandat 1631.197 kb on + strandat 1631.624 kb on - strand, within thrCat 1631.701 kb on - strand, within thrCat 1631.820 kb on + strand, within thrCat 1631.830 kb on - strand, within thrCat 1632.295 kb on + strand, within thrCat 1632.296 kb on - strand, within thrCat 1632.404 kb on + strandat 1632.404 kb on + strandat 1632.420 kb on + strandat 1632.420 kb on + strandat 1632.472 kb on + strandat 1632.479 kb on + strandat 1632.487 kb on - strandat 1632.512 kb on + strandat 1632.594 kb on + strandat 1632.673 kb on + strandat 1632.674 kb on - strandat 1632.705 kb on + strandat 1632.706 kb on - strandat 1632.706 kb on - strandat 1632.710 kb on + strandat 1632.765 kb on + strand, within yaaXat 1632.765 kb on + strand, within yaaXat 1632.795 kb on - strand, within yaaXat 1632.820 kb on + strand, within yaaXat 1632.820 kb on + strand, within yaaXat 1632.868 kb on + strand, within yaaXat 1632.868 kb on + strand, within yaaXat 1632.928 kb on - strand, within yaaXat 1632.982 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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1,630,193 + +0.1
1,630,222 + +1.3
1,630,222 + -0.1
1,630,223 - -0.9
1,630,226 + -1.7
1,630,226 + -2.9
1,630,244 - -2.7
1,630,260 + -2.3
1,630,358 + thrB BNILDI_08165 0.11 +1.9
1,630,358 + thrB BNILDI_08165 0.11 +0.0
1,630,358 + thrB BNILDI_08165 0.11 +2.1
1,630,358 + thrB BNILDI_08165 0.11 +2.0
1,630,359 - thrB BNILDI_08165 0.11 -1.8
1,630,359 - thrB BNILDI_08165 0.11 +0.1
1,630,359 - thrB BNILDI_08165 0.11 -1.1
1,630,443 + thrB BNILDI_08165 0.20 -0.6
1,630,444 - thrB BNILDI_08165 0.20 -0.0
1,630,522 - thrB BNILDI_08165 0.29 +0.1
1,630,542 - thrB BNILDI_08165 0.31 +0.2
1,630,821 + thrB BNILDI_08165 0.61 +1.4
1,630,897 + thrB BNILDI_08165 0.69 -0.7
1,630,941 + thrB BNILDI_08165 0.74 -2.7
1,630,954 + thrB BNILDI_08165 0.75 -0.4
1,631,061 - thrB BNILDI_08165 0.86 -0.4
1,631,114 - +0.1
1,631,143 - +0.3
1,631,197 + -1.5
1,631,624 - thrC BNILDI_08170 0.34 -0.7
1,631,701 - thrC BNILDI_08170 0.40 +0.6
1,631,820 + thrC BNILDI_08170 0.49 -2.5
1,631,830 - thrC BNILDI_08170 0.50 +0.4
1,632,295 + thrC BNILDI_08170 0.86 -1.6
1,632,296 - thrC BNILDI_08170 0.86 +0.8
1,632,404 + +1.4
1,632,404 + +1.6
1,632,420 + -0.3
1,632,420 + +2.4
1,632,472 + +0.9
1,632,479 + +1.9
1,632,487 - -1.2
1,632,512 + +1.2
1,632,594 + -0.6
1,632,673 + +1.1
1,632,674 - -0.8
1,632,705 + +1.4
1,632,706 - -1.5
1,632,706 - +0.5
1,632,710 + -1.7
1,632,765 + yaaX BNILDI_08175 0.26 -0.2
1,632,765 + yaaX BNILDI_08175 0.26 +0.7
1,632,795 - yaaX BNILDI_08175 0.36 -0.0
1,632,820 + yaaX BNILDI_08175 0.44 -0.8
1,632,820 + yaaX BNILDI_08175 0.44 +0.1
1,632,868 + yaaX BNILDI_08175 0.61 +0.4
1,632,868 + yaaX BNILDI_08175 0.61 +0.5
1,632,928 - yaaX BNILDI_08175 0.81 +1.7
1,632,982 + +1.1

Or see this region's nucleotide sequence