Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcsA and bcsQ overlap by 4 nucleotides bcsQ and bcsR are separated by 11 nucleotides bcsR and bcsE are separated by 284 nucleotides
BNILDI_03420: bcsA - UDP-forming cellulose synthase catalytic subunit, at 672,324 to 674,942
bcsA
BNILDI_03425: bcsQ - cellulose biosynthesis protein BcsQ, at 674,939 to 675,691
bcsQ
BNILDI_03430: bcsR - cellulose biosynthesis protein BcsR, at 675,703 to 675,891
bcsR
BNILDI_03435: bcsE - cellulose biosynthesis protein BcsE, at 676,176 to 677,735
bcsE
Position (kb) 674
675
676 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 673.953 kb on - strand, within bcsA at 673.954 kb on + strand, within bcsA at 674.062 kb on + strand, within bcsA at 674.062 kb on + strand, within bcsA at 674.066 kb on + strand, within bcsA at 674.098 kb on + strand, within bcsA at 674.099 kb on - strand, within bcsA at 674.114 kb on + strand, within bcsA at 674.138 kb on + strand, within bcsA at 674.139 kb on - strand, within bcsA at 674.139 kb on - strand, within bcsA at 674.139 kb on - strand, within bcsA at 674.139 kb on - strand, within bcsA at 674.179 kb on - strand, within bcsA at 674.218 kb on + strand, within bcsA at 674.290 kb on + strand, within bcsA at 674.290 kb on + strand, within bcsA at 674.303 kb on + strand, within bcsA at 674.304 kb on - strand, within bcsA at 674.305 kb on + strand, within bcsA at 674.306 kb on - strand, within bcsA at 674.306 kb on - strand, within bcsA at 674.373 kb on + strand, within bcsA at 674.411 kb on + strand, within bcsA at 674.412 kb on - strand, within bcsA at 674.667 kb on - strand, within bcsA at 674.746 kb on + strand at 674.757 kb on - strand at 674.977 kb on - strand at 675.008 kb on + strand at 675.009 kb on - strand at 675.142 kb on - strand, within bcsQ at 675.146 kb on - strand, within bcsQ at 675.190 kb on + strand, within bcsQ at 675.191 kb on - strand, within bcsQ at 675.191 kb on - strand, within bcsQ at 675.326 kb on + strand, within bcsQ at 675.547 kb on + strand, within bcsQ at 675.547 kb on + strand, within bcsQ at 675.548 kb on - strand, within bcsQ at 675.548 kb on - strand, within bcsQ at 675.828 kb on + strand, within bcsR at 675.870 kb on + strand, within bcsR at 675.870 kb on + strand, within bcsR at 675.913 kb on - strand at 675.919 kb on - strand at 676.117 kb on - strand at 676.123 kb on + strand at 676.186 kb on + strand at 676.199 kb on + strand at 676.211 kb on - strand at 676.229 kb on + strand at 676.230 kb on - strand at 676.280 kb on + strand at 676.443 kb on + strand, within bcsE at 676.469 kb on + strand, within bcsE at 676.470 kb on - strand, within bcsE
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 673,953 - bcsA BNILDI_03420 0.62 -0.2 673,954 + bcsA BNILDI_03420 0.62 -2.6 674,062 + bcsA BNILDI_03420 0.66 -2.2 674,062 + bcsA BNILDI_03420 0.66 -0.1 674,066 + bcsA BNILDI_03420 0.67 +0.9 674,098 + bcsA BNILDI_03420 0.68 +0.8 674,099 - bcsA BNILDI_03420 0.68 -0.7 674,114 + bcsA BNILDI_03420 0.68 -0.0 674,138 + bcsA BNILDI_03420 0.69 +0.4 674,139 - bcsA BNILDI_03420 0.69 -1.3 674,139 - bcsA BNILDI_03420 0.69 +0.9 674,139 - bcsA BNILDI_03420 0.69 -2.5 674,139 - bcsA BNILDI_03420 0.69 +0.0 674,179 - bcsA BNILDI_03420 0.71 -0.0 674,218 + bcsA BNILDI_03420 0.72 +1.3 674,290 + bcsA BNILDI_03420 0.75 -0.5 674,290 + bcsA BNILDI_03420 0.75 -1.5 674,303 + bcsA BNILDI_03420 0.76 -1.2 674,304 - bcsA BNILDI_03420 0.76 +0.1 674,305 + bcsA BNILDI_03420 0.76 +0.3 674,306 - bcsA BNILDI_03420 0.76 +1.3 674,306 - bcsA BNILDI_03420 0.76 +0.2 674,373 + bcsA BNILDI_03420 0.78 -0.7 674,411 + bcsA BNILDI_03420 0.80 -0.9 674,412 - bcsA BNILDI_03420 0.80 +0.2 674,667 - bcsA BNILDI_03420 0.89 -2.0 674,746 + +3.6 674,757 - +0.1 674,977 - -0.4 675,008 + +0.2 675,009 - +0.5 675,142 - bcsQ BNILDI_03425 0.27 +0.2 675,146 - bcsQ BNILDI_03425 0.27 -0.9 675,190 + bcsQ BNILDI_03425 0.33 -0.5 675,191 - bcsQ BNILDI_03425 0.33 -1.4 675,191 - bcsQ BNILDI_03425 0.33 -1.7 675,326 + bcsQ BNILDI_03425 0.51 +0.5 675,547 + bcsQ BNILDI_03425 0.81 -1.0 675,547 + bcsQ BNILDI_03425 0.81 -0.0 675,548 - bcsQ BNILDI_03425 0.81 +0.0 675,548 - bcsQ BNILDI_03425 0.81 -0.5 675,828 + bcsR BNILDI_03430 0.66 +1.7 675,870 + bcsR BNILDI_03430 0.88 -1.5 675,870 + bcsR BNILDI_03430 0.88 +2.3 675,913 - -0.9 675,919 - +0.5 676,117 - -0.8 676,123 + -0.5 676,186 + -1.0 676,199 + +0.8 676,211 - -1.2 676,229 + +1.3 676,230 - -0.3 676,280 + +2.8 676,443 + bcsE BNILDI_03435 0.17 +0.9 676,469 + bcsE BNILDI_03435 0.19 +1.3 676,470 - bcsE BNILDI_03435 0.19 -2.0
Or see this region's nucleotide sequence