Strain Fitness in Escherichia coli ECRC62 around BNILDI_01685

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsH and rlmE are separated by 90 nucleotidesrlmE and yhbY are separated by 125 nucleotidesyhbY and greA are separated by 155 nucleotidesgreA and dacB are separated by 247 nucleotides BNILDI_01675: ftsH - ATP-dependent zinc metalloprotease FtsH, at 329,522 to 331,465 ftsH BNILDI_01680: rlmE - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 331,556 to 332,185 rlmE BNILDI_01685: yhbY - ribosome assembly RNA-binding protein YhbY, at 332,311 to 332,604 yhbY BNILDI_01690: greA - transcription elongation factor GreA, at 332,760 to 333,236 greA BNILDI_01695: dacB - serine-type D-Ala-D-Ala carboxypeptidase, at 333,484 to 334,917 dacB Position (kb) 332 333Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 332.177 kb on - strandat 332.253 kb on - strandat 332.253 kb on - strandat 332.253 kb on - strandat 332.253 kb on - strandat 332.253 kb on - strandat 332.255 kb on - strandat 332.325 kb on + strandat 332.326 kb on - strandat 332.388 kb on + strand, within yhbYat 332.432 kb on + strand, within yhbYat 332.485 kb on - strand, within yhbYat 332.506 kb on - strand, within yhbYat 332.506 kb on - strand, within yhbYat 332.529 kb on + strand, within yhbYat 332.529 kb on + strand, within yhbYat 332.534 kb on + strand, within yhbYat 332.535 kb on - strand, within yhbYat 332.535 kb on - strand, within yhbYat 332.574 kb on + strand, within yhbYat 332.574 kb on + strand, within yhbYat 332.636 kb on + strandat 332.692 kb on + strandat 332.777 kb on + strandat 332.778 kb on - strandat 332.778 kb on - strandat 332.781 kb on + strandat 332.782 kb on - strandat 332.794 kb on - strandat 332.892 kb on - strand, within greAat 332.924 kb on - strand, within greAat 333.094 kb on + strand, within greAat 333.241 kb on + strandat 333.241 kb on + strandat 333.242 kb on - strandat 333.243 kb on + strandat 333.244 kb on - strandat 333.244 kb on - strandat 333.424 kb on - strandat 333.431 kb on - strandat 333.445 kb on + strandat 333.448 kb on + strandat 333.522 kb on + strandat 333.523 kb on - strandat 333.523 kb on - strandat 333.524 kb on + strandat 333.559 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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332,177 - +0.8
332,253 - +0.6
332,253 - +2.6
332,253 - +0.6
332,253 - +1.5
332,253 - +4.4
332,255 - +1.8
332,325 + +1.4
332,326 - +1.9
332,388 + yhbY BNILDI_01685 0.26 -0.2
332,432 + yhbY BNILDI_01685 0.41 +3.1
332,485 - yhbY BNILDI_01685 0.59 +0.0
332,506 - yhbY BNILDI_01685 0.66 -0.4
332,506 - yhbY BNILDI_01685 0.66 -0.0
332,529 + yhbY BNILDI_01685 0.74 +3.2
332,529 + yhbY BNILDI_01685 0.74 -1.2
332,534 + yhbY BNILDI_01685 0.76 +1.5
332,535 - yhbY BNILDI_01685 0.76 +2.4
332,535 - yhbY BNILDI_01685 0.76 -0.0
332,574 + yhbY BNILDI_01685 0.89 -0.5
332,574 + yhbY BNILDI_01685 0.89 -0.5
332,636 + +1.0
332,692 + +0.5
332,777 + -4.0
332,778 - -4.6
332,778 - -3.6
332,781 + -1.5
332,782 - -4.0
332,794 - -0.2
332,892 - greA BNILDI_01690 0.28 -0.2
332,924 - greA BNILDI_01690 0.34 -2.3
333,094 + greA BNILDI_01690 0.70 +0.4
333,241 + +1.4
333,241 + +2.4
333,242 - -2.3
333,243 + -2.1
333,244 - -3.0
333,244 - -1.0
333,424 - +1.2
333,431 - -1.0
333,445 + -0.4
333,448 + -2.3
333,522 + -1.8
333,523 - +1.2
333,523 - -1.3
333,524 + +2.7
333,559 + -1.4

Or see this region's nucleotide sequence