Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfaD and glpC are separated by 192 nucleotides glpC and glpB overlap by 4 nucleotides
HEPCGN_22290: yfaD - ISNCY family transposase, at 3,215,169 to 3,216,104
yfaD
HEPCGN_22295: glpC - anaerobic glycerol-3-phosphate dehydrogenase subunit GlpC, at 3,216,297 to 3,217,487
glpC
HEPCGN_22300: glpB - glycerol-3-phosphate dehydrogenase subunit GlpB, at 3,217,484 to 3,218,743
glpB
Position (kb)
3216
3217
3218 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3215.370 kb on - strand, within yfaD at 3215.370 kb on - strand, within yfaD at 3215.376 kb on + strand, within yfaD at 3215.391 kb on + strand, within yfaD at 3215.392 kb on - strand, within yfaD at 3215.465 kb on + strand, within yfaD at 3215.465 kb on + strand, within yfaD at 3215.465 kb on + strand, within yfaD at 3215.466 kb on - strand, within yfaD at 3215.478 kb on + strand, within yfaD at 3215.586 kb on + strand, within yfaD at 3215.586 kb on + strand, within yfaD at 3215.588 kb on + strand, within yfaD at 3215.620 kb on - strand, within yfaD at 3215.656 kb on + strand, within yfaD at 3215.737 kb on + strand, within yfaD at 3215.872 kb on + strand, within yfaD at 3215.873 kb on - strand, within yfaD at 3215.873 kb on - strand, within yfaD at 3215.877 kb on - strand, within yfaD at 3215.878 kb on + strand, within yfaD at 3216.009 kb on + strand, within yfaD at 3216.010 kb on - strand, within yfaD at 3216.015 kb on + strand at 3216.016 kb on - strand at 3216.054 kb on - strand at 3216.123 kb on - strand at 3216.123 kb on - strand at 3216.126 kb on + strand at 3216.142 kb on + strand at 3216.225 kb on - strand at 3216.226 kb on + strand at 3216.324 kb on - strand at 3216.372 kb on - strand at 3216.481 kb on + strand, within glpC at 3216.770 kb on + strand, within glpC at 3216.938 kb on + strand, within glpC at 3216.939 kb on - strand, within glpC at 3216.970 kb on + strand, within glpC at 3216.970 kb on + strand, within glpC at 3216.971 kb on - strand, within glpC at 3216.971 kb on - strand, within glpC at 3217.010 kb on + strand, within glpC at 3217.011 kb on - strand, within glpC at 3217.011 kb on - strand, within glpC at 3217.012 kb on + strand, within glpC at 3217.012 kb on + strand, within glpC at 3217.012 kb on + strand, within glpC at 3217.012 kb on + strand, within glpC at 3217.343 kb on - strand, within glpC at 3217.458 kb on - strand at 3217.529 kb on + strand at 3217.530 kb on - strand at 3217.530 kb on - strand at 3217.736 kb on + strand, within glpB at 3217.737 kb on - strand, within glpB at 3217.880 kb on + strand, within glpB at 3217.880 kb on + strand, within glpB at 3218.028 kb on - strand, within glpB at 3218.028 kb on - strand, within glpB at 3218.120 kb on - strand, within glpB at 3218.132 kb on + strand, within glpB at 3218.133 kb on - strand, within glpB at 3218.319 kb on - strand, within glpB at 3218.321 kb on + strand, within glpB at 3218.327 kb on + strand, within glpB at 3218.372 kb on + strand, within glpB at 3218.462 kb on + strand, within glpB
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 3,215,370 - yfaD HEPCGN_22290 0.21 +0.3 3,215,370 - yfaD HEPCGN_22290 0.21 -1.0 3,215,376 + yfaD HEPCGN_22290 0.22 -0.3 3,215,391 + yfaD HEPCGN_22290 0.24 -0.2 3,215,392 - yfaD HEPCGN_22290 0.24 -0.1 3,215,465 + yfaD HEPCGN_22290 0.32 +0.6 3,215,465 + yfaD HEPCGN_22290 0.32 +0.6 3,215,465 + yfaD HEPCGN_22290 0.32 +0.4 3,215,466 - yfaD HEPCGN_22290 0.32 -0.6 3,215,478 + yfaD HEPCGN_22290 0.33 -0.8 3,215,586 + yfaD HEPCGN_22290 0.45 +0.6 3,215,586 + yfaD HEPCGN_22290 0.45 -0.1 3,215,588 + yfaD HEPCGN_22290 0.45 -0.2 3,215,620 - yfaD HEPCGN_22290 0.48 +2.0 3,215,656 + yfaD HEPCGN_22290 0.52 -0.4 3,215,737 + yfaD HEPCGN_22290 0.61 +0.7 3,215,872 + yfaD HEPCGN_22290 0.75 -0.2 3,215,873 - yfaD HEPCGN_22290 0.75 +0.9 3,215,873 - yfaD HEPCGN_22290 0.75 -0.6 3,215,877 - yfaD HEPCGN_22290 0.76 +1.9 3,215,878 + yfaD HEPCGN_22290 0.76 +0.6 3,216,009 + yfaD HEPCGN_22290 0.90 +0.0 3,216,010 - yfaD HEPCGN_22290 0.90 +0.1 3,216,015 + -0.6 3,216,016 - -0.1 3,216,054 - +0.8 3,216,123 - -0.5 3,216,123 - -0.7 3,216,126 + +0.8 3,216,142 + +0.6 3,216,225 - +0.1 3,216,226 + +0.7 3,216,324 - +0.2 3,216,372 - +2.6 3,216,481 + glpC HEPCGN_22295 0.15 +0.6 3,216,770 + glpC HEPCGN_22295 0.40 +0.9 3,216,938 + glpC HEPCGN_22295 0.54 -0.2 3,216,939 - glpC HEPCGN_22295 0.54 -2.8 3,216,970 + glpC HEPCGN_22295 0.57 -0.4 3,216,970 + glpC HEPCGN_22295 0.57 -0.4 3,216,971 - glpC HEPCGN_22295 0.57 -0.1 3,216,971 - glpC HEPCGN_22295 0.57 +0.1 3,217,010 + glpC HEPCGN_22295 0.60 -0.6 3,217,011 - glpC HEPCGN_22295 0.60 +0.1 3,217,011 - glpC HEPCGN_22295 0.60 -1.0 3,217,012 + glpC HEPCGN_22295 0.60 -2.0 3,217,012 + glpC HEPCGN_22295 0.60 -0.8 3,217,012 + glpC HEPCGN_22295 0.60 -1.6 3,217,012 + glpC HEPCGN_22295 0.60 +0.6 3,217,343 - glpC HEPCGN_22295 0.88 +0.4 3,217,458 - -0.1 3,217,529 + -0.9 3,217,530 - -0.1 3,217,530 - +2.5 3,217,736 + glpB HEPCGN_22300 0.20 +0.7 3,217,737 - glpB HEPCGN_22300 0.20 -0.8 3,217,880 + glpB HEPCGN_22300 0.31 -2.4 3,217,880 + glpB HEPCGN_22300 0.31 -0.5 3,218,028 - glpB HEPCGN_22300 0.43 +0.4 3,218,028 - glpB HEPCGN_22300 0.43 +0.5 3,218,120 - glpB HEPCGN_22300 0.50 -1.0 3,218,132 + glpB HEPCGN_22300 0.51 +0.5 3,218,133 - glpB HEPCGN_22300 0.52 +1.0 3,218,319 - glpB HEPCGN_22300 0.66 +0.3 3,218,321 + glpB HEPCGN_22300 0.66 +1.5 3,218,327 + glpB HEPCGN_22300 0.67 -1.2 3,218,372 + glpB HEPCGN_22300 0.70 +0.3 3,218,462 + glpB HEPCGN_22300 0.78 -0.5
Or see this region's nucleotide sequence