Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iscU and iscA are separated by 16 nucleotides iscA and hscB are separated by 95 nucleotides hscB and hscA are separated by 2 nucleotides hscA and fdx are separated by 1 nucleotides fdx and iscX are separated by 11 nucleotides iscX and pepB are separated by 58 nucleotides
HEPCGN_20765: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,893,777 to 2,894,163
iscU
HEPCGN_20770: iscA - iron-sulfur cluster assembly protein IscA, at 2,894,180 to 2,894,503
iscA
HEPCGN_20775: hscB - co-chaperone HscB, at 2,894,599 to 2,895,129
hscB
HEPCGN_20780: hscA - Fe-S protein assembly chaperone HscA, at 2,895,132 to 2,896,982
hscA
HEPCGN_20785: fdx - ISC system 2Fe-2S type ferredoxin, at 2,896,984 to 2,897,319
fdx
HEPCGN_20790: iscX - Fe-S cluster assembly protein IscX, at 2,897,331 to 2,897,531
iscX
HEPCGN_20795: pepB - aminopeptidase PepB, at 2,897,590 to 2,898,873
pepB
Position (kb)
2895
2896
2897 Strain fitness (log2 ratio)
-1
0
1 at 2894.221 kb on + strand, within iscA at 2894.297 kb on + strand, within iscA at 2894.298 kb on - strand, within iscA at 2894.418 kb on + strand, within iscA at 2894.431 kb on + strand, within iscA at 2894.599 kb on - strand at 2894.647 kb on - strand at 2894.851 kb on - strand, within hscB at 2895.005 kb on + strand, within hscB at 2895.306 kb on + strand at 2895.344 kb on + strand, within hscA at 2895.874 kb on - strand, within hscA at 2896.102 kb on + strand, within hscA at 2896.368 kb on - strand, within hscA at 2896.433 kb on + strand, within hscA at 2896.442 kb on - strand, within hscA at 2896.670 kb on + strand, within hscA at 2896.686 kb on + strand, within hscA at 2896.983 kb on + strand at 2897.046 kb on + strand, within fdx at 2897.047 kb on - strand, within fdx at 2897.049 kb on + strand, within fdx at 2897.295 kb on + strand at 2897.328 kb on - strand at 2897.328 kb on - strand at 2897.330 kb on + strand at 2897.330 kb on + strand at 2897.339 kb on + strand at 2897.339 kb on + strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.376 kb on + strand, within iscX at 2897.376 kb on + strand, within iscX at 2897.377 kb on - strand, within iscX at 2897.529 kb on + strand at 2897.586 kb on + strand at 2897.587 kb on - strand at 2897.611 kb on - strand at 2897.659 kb on + strand at 2897.660 kb on - strand at 2897.667 kb on + strand at 2897.667 kb on + strand at 2897.670 kb on + strand at 2897.671 kb on - strand at 2897.683 kb on - strand at 2897.683 kb on - strand at 2897.821 kb on + strand, within pepB at 2897.821 kb on + strand, within pepB at 2897.845 kb on - strand, within pepB
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,894,221 + iscA HEPCGN_20770 0.13 +0.0 2,894,297 + iscA HEPCGN_20770 0.36 -1.2 2,894,298 - iscA HEPCGN_20770 0.36 +0.6 2,894,418 + iscA HEPCGN_20770 0.73 +0.3 2,894,431 + iscA HEPCGN_20770 0.77 +0.6 2,894,599 - -0.4 2,894,647 - +0.6 2,894,851 - hscB HEPCGN_20775 0.47 +0.6 2,895,005 + hscB HEPCGN_20775 0.76 -0.7 2,895,306 + +0.6 2,895,344 + hscA HEPCGN_20780 0.11 +0.0 2,895,874 - hscA HEPCGN_20780 0.40 +0.0 2,896,102 + hscA HEPCGN_20780 0.52 -0.7 2,896,368 - hscA HEPCGN_20780 0.67 +1.2 2,896,433 + hscA HEPCGN_20780 0.70 +0.6 2,896,442 - hscA HEPCGN_20780 0.71 -0.4 2,896,670 + hscA HEPCGN_20780 0.83 +0.6 2,896,686 + hscA HEPCGN_20780 0.84 +0.6 2,896,983 + -0.4 2,897,046 + fdx HEPCGN_20785 0.18 +0.6 2,897,047 - fdx HEPCGN_20785 0.19 +0.6 2,897,049 + fdx HEPCGN_20785 0.19 +1.6 2,897,295 + -1.4 2,897,328 - -0.3 2,897,328 - -0.9 2,897,330 + +1.0 2,897,330 + -0.4 2,897,339 + -0.0 2,897,339 + +0.7 2,897,340 - -0.0 2,897,340 - +0.1 2,897,340 - -0.4 2,897,340 - -0.5 2,897,376 + iscX HEPCGN_20790 0.22 -0.4 2,897,376 + iscX HEPCGN_20790 0.22 +1.0 2,897,377 - iscX HEPCGN_20790 0.23 +0.0 2,897,529 + -0.1 2,897,586 + -0.4 2,897,587 - -0.6 2,897,611 - +0.2 2,897,659 + +0.5 2,897,660 - +0.0 2,897,667 + +0.1 2,897,667 + -1.0 2,897,670 + -0.3 2,897,671 - +0.4 2,897,683 - +1.6 2,897,683 - +0.8 2,897,821 + pepB HEPCGN_20795 0.18 -0.1 2,897,821 + pepB HEPCGN_20795 0.18 +0.2 2,897,845 - pepB HEPCGN_20795 0.20 -0.1
Or see this region's nucleotide sequence