Strain Fitness in Escherichia coli ECOR38 around HEPCGN_19280

Experiment: RB68

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntthyA and ppdA are separated by 184 nucleotidesppdA and pulJ overlap by 10 nucleotidespulJ and ygdB overlap by 4 nucleotidesygdB and ppdC overlap by 16 nucleotidesppdC and recC are separated by 12 nucleotides HEPCGN_19270: thyA - thymidylate synthase, at 2,605,257 to 2,606,051 thyA HEPCGN_19275: ppdA - prepilin peptidase-dependent protein, at 2,606,236 to 2,606,706 ppdA HEPCGN_19280: pulJ - prepilin peptidase-dependent protein, at 2,606,697 to 2,607,260 pulJ HEPCGN_19285: ygdB - DUF2509 domain-containing protein, at 2,607,257 to 2,607,664 ygdB HEPCGN_19290: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,607,649 to 2,607,972 ppdC HEPCGN_19295: recC - exodeoxyribonuclease V subunit gamma, at 2,607,985 to 2,611,353 recC Position (kb) 2606 2607 2608Strain fitness (log2 ratio) -2 -1 0 1 2at 2605.892 kb on + strand, within thyAat 2606.049 kb on + strandat 2606.050 kb on - strandat 2606.050 kb on - strandat 2606.050 kb on - strandat 2606.053 kb on + strandat 2606.164 kb on + strandat 2606.165 kb on - strandat 2606.165 kb on - strandat 2606.226 kb on + strandat 2606.226 kb on + strandat 2606.256 kb on + strandat 2606.301 kb on - strand, within ppdAat 2606.301 kb on - strand, within ppdAat 2606.430 kb on + strand, within ppdAat 2606.430 kb on + strand, within ppdAat 2606.431 kb on - strand, within ppdAat 2606.761 kb on + strand, within pulJat 2606.761 kb on + strand, within pulJat 2606.762 kb on - strand, within pulJat 2606.762 kb on - strand, within pulJat 2606.797 kb on + strand, within pulJat 2606.798 kb on - strand, within pulJat 2606.825 kb on + strand, within pulJat 2606.895 kb on + strand, within pulJat 2607.185 kb on + strand, within pulJat 2607.186 kb on - strand, within pulJat 2607.186 kb on - strand, within pulJat 2607.298 kb on + strand, within ygdBat 2607.325 kb on - strand, within ygdBat 2607.476 kb on + strand, within ygdBat 2607.510 kb on + strand, within ygdBat 2607.510 kb on + strand, within ygdBat 2607.532 kb on - strand, within ygdBat 2607.758 kb on + strand, within ppdCat 2607.758 kb on + strand, within ppdCat 2607.758 kb on + strand, within ppdCat 2607.912 kb on - strand, within ppdCat 2607.912 kb on - strand, within ppdCat 2607.970 kb on + strandat 2607.984 kb on + strandat 2607.984 kb on + strandat 2607.984 kb on + strandat 2608.101 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RB68
remove
2,605,892 + thyA HEPCGN_19270 0.80 +1.7
2,606,049 + -0.3
2,606,050 - -0.1
2,606,050 - -1.4
2,606,050 - +1.2
2,606,053 + +2.2
2,606,164 + -0.6
2,606,165 - +1.1
2,606,165 - +0.2
2,606,226 + -0.2
2,606,226 + +0.3
2,606,256 + -0.1
2,606,301 - ppdA HEPCGN_19275 0.14 +1.4
2,606,301 - ppdA HEPCGN_19275 0.14 +0.5
2,606,430 + ppdA HEPCGN_19275 0.41 +0.3
2,606,430 + ppdA HEPCGN_19275 0.41 +1.6
2,606,431 - ppdA HEPCGN_19275 0.41 -0.3
2,606,761 + pulJ HEPCGN_19280 0.11 -0.4
2,606,761 + pulJ HEPCGN_19280 0.11 -0.1
2,606,762 - pulJ HEPCGN_19280 0.12 -0.4
2,606,762 - pulJ HEPCGN_19280 0.12 +0.1
2,606,797 + pulJ HEPCGN_19280 0.18 -0.3
2,606,798 - pulJ HEPCGN_19280 0.18 +0.2
2,606,825 + pulJ HEPCGN_19280 0.23 +0.8
2,606,895 + pulJ HEPCGN_19280 0.35 +0.0
2,607,185 + pulJ HEPCGN_19280 0.87 -0.1
2,607,186 - pulJ HEPCGN_19280 0.87 +0.3
2,607,186 - pulJ HEPCGN_19280 0.87 +0.9
2,607,298 + ygdB HEPCGN_19285 0.10 -1.1
2,607,325 - ygdB HEPCGN_19285 0.17 +0.6
2,607,476 + ygdB HEPCGN_19285 0.54 -0.9
2,607,510 + ygdB HEPCGN_19285 0.62 -0.3
2,607,510 + ygdB HEPCGN_19285 0.62 +1.7
2,607,532 - ygdB HEPCGN_19285 0.67 +1.9
2,607,758 + ppdC HEPCGN_19290 0.34 +0.6
2,607,758 + ppdC HEPCGN_19290 0.34 +1.7
2,607,758 + ppdC HEPCGN_19290 0.34 +1.7
2,607,912 - ppdC HEPCGN_19290 0.81 -1.9
2,607,912 - ppdC HEPCGN_19290 0.81 +1.7
2,607,970 + -1.0
2,607,984 + -0.6
2,607,984 + +2.0
2,607,984 + +0.4
2,608,101 + +0.1

Or see this region's nucleotide sequence