Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thyA and ppdA are separated by 184 nucleotides ppdA and pulJ overlap by 10 nucleotides pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides
HEPCGN_19270: thyA - thymidylate synthase, at 2,605,257 to 2,606,051
thyA
HEPCGN_19275: ppdA - prepilin peptidase-dependent protein, at 2,606,236 to 2,606,706
ppdA
HEPCGN_19280: pulJ - prepilin peptidase-dependent protein, at 2,606,697 to 2,607,260
pulJ
HEPCGN_19285: ygdB - DUF2509 domain-containing protein, at 2,607,257 to 2,607,664
ygdB
HEPCGN_19290: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,607,649 to 2,607,972
ppdC
HEPCGN_19295: recC - exodeoxyribonuclease V subunit gamma, at 2,607,985 to 2,611,353
recC
Position (kb)
2606
2607
2608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2605.892 kb on + strand, within thyA at 2606.049 kb on + strand at 2606.050 kb on - strand at 2606.050 kb on - strand at 2606.050 kb on - strand at 2606.053 kb on + strand at 2606.164 kb on + strand at 2606.165 kb on - strand at 2606.165 kb on - strand at 2606.226 kb on + strand at 2606.226 kb on + strand at 2606.256 kb on + strand at 2606.301 kb on - strand, within ppdA at 2606.301 kb on - strand, within ppdA at 2606.430 kb on + strand, within ppdA at 2606.430 kb on + strand, within ppdA at 2606.431 kb on - strand, within ppdA at 2606.761 kb on + strand, within pulJ at 2606.761 kb on + strand, within pulJ at 2606.762 kb on - strand, within pulJ at 2606.762 kb on - strand, within pulJ at 2606.797 kb on + strand, within pulJ at 2606.798 kb on - strand, within pulJ at 2606.825 kb on + strand, within pulJ at 2606.895 kb on + strand, within pulJ at 2607.185 kb on + strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.298 kb on + strand, within ygdB at 2607.325 kb on - strand, within ygdB at 2607.476 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.532 kb on - strand, within ygdB at 2607.758 kb on + strand, within ppdC at 2607.758 kb on + strand, within ppdC at 2607.758 kb on + strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.970 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2608.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,605,892 + thyA HEPCGN_19270 0.80 +1.7 2,606,049 + -0.3 2,606,050 - -0.1 2,606,050 - -1.4 2,606,050 - +1.2 2,606,053 + +2.2 2,606,164 + -0.6 2,606,165 - +1.1 2,606,165 - +0.2 2,606,226 + -0.2 2,606,226 + +0.3 2,606,256 + -0.1 2,606,301 - ppdA HEPCGN_19275 0.14 +1.4 2,606,301 - ppdA HEPCGN_19275 0.14 +0.5 2,606,430 + ppdA HEPCGN_19275 0.41 +0.3 2,606,430 + ppdA HEPCGN_19275 0.41 +1.6 2,606,431 - ppdA HEPCGN_19275 0.41 -0.3 2,606,761 + pulJ HEPCGN_19280 0.11 -0.4 2,606,761 + pulJ HEPCGN_19280 0.11 -0.1 2,606,762 - pulJ HEPCGN_19280 0.12 -0.4 2,606,762 - pulJ HEPCGN_19280 0.12 +0.1 2,606,797 + pulJ HEPCGN_19280 0.18 -0.3 2,606,798 - pulJ HEPCGN_19280 0.18 +0.2 2,606,825 + pulJ HEPCGN_19280 0.23 +0.8 2,606,895 + pulJ HEPCGN_19280 0.35 +0.0 2,607,185 + pulJ HEPCGN_19280 0.87 -0.1 2,607,186 - pulJ HEPCGN_19280 0.87 +0.3 2,607,186 - pulJ HEPCGN_19280 0.87 +0.9 2,607,298 + ygdB HEPCGN_19285 0.10 -1.1 2,607,325 - ygdB HEPCGN_19285 0.17 +0.6 2,607,476 + ygdB HEPCGN_19285 0.54 -0.9 2,607,510 + ygdB HEPCGN_19285 0.62 -0.3 2,607,510 + ygdB HEPCGN_19285 0.62 +1.7 2,607,532 - ygdB HEPCGN_19285 0.67 +1.9 2,607,758 + ppdC HEPCGN_19290 0.34 +0.6 2,607,758 + ppdC HEPCGN_19290 0.34 +1.7 2,607,758 + ppdC HEPCGN_19290 0.34 +1.7 2,607,912 - ppdC HEPCGN_19290 0.81 -1.9 2,607,912 - ppdC HEPCGN_19290 0.81 +1.7 2,607,970 + -1.0 2,607,984 + -0.6 2,607,984 + +2.0 2,607,984 + +0.4 2,608,101 + +0.1
Or see this region's nucleotide sequence