Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiI and gcvT are separated by 447 nucleotides gcvT and gcvH are separated by 23 nucleotides gcvH and gcvP are separated by 117 nucleotides
HEPCGN_18805: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 2,503,884 to 2,505,086
ubiI
HEPCGN_18810: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 2,505,534 to 2,506,628
gcvT
HEPCGN_18815: gcvH - glycine cleavage system protein GcvH, at 2,506,652 to 2,507,041
gcvH
HEPCGN_18820: gcvP - aminomethyl-transferring glycine dehydrogenase, at 2,507,159 to 2,510,032
gcvP
Position (kb)
2505
2506
2507 Strain fitness (log2 ratio)
-1
0
1
2 at 2504.660 kb on - strand, within ubiI at 2504.930 kb on + strand, within ubiI at 2505.106 kb on + strand at 2505.175 kb on + strand at 2505.176 kb on - strand at 2505.276 kb on + strand at 2505.555 kb on + strand at 2505.556 kb on - strand at 2505.621 kb on + strand at 2505.622 kb on - strand at 2505.622 kb on - strand at 2505.657 kb on - strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.686 kb on + strand, within gcvT at 2505.687 kb on - strand, within gcvT at 2505.687 kb on - strand, within gcvT at 2505.687 kb on - strand, within gcvT at 2505.736 kb on - strand, within gcvT at 2505.820 kb on + strand, within gcvT at 2505.821 kb on - strand, within gcvT at 2505.837 kb on + strand, within gcvT at 2505.837 kb on + strand, within gcvT at 2505.841 kb on - strand, within gcvT at 2505.846 kb on - strand, within gcvT at 2506.014 kb on - strand, within gcvT at 2506.099 kb on - strand, within gcvT at 2506.100 kb on + strand, within gcvT at 2506.100 kb on + strand, within gcvT at 2506.240 kb on - strand, within gcvT at 2506.500 kb on + strand, within gcvT at 2506.500 kb on + strand, within gcvT at 2506.500 kb on + strand, within gcvT at 2506.501 kb on - strand, within gcvT at 2506.533 kb on - strand at 2506.613 kb on + strand at 2506.662 kb on + strand at 2506.680 kb on - strand at 2506.724 kb on + strand, within gcvH at 2506.725 kb on - strand, within gcvH at 2506.843 kb on - strand, within gcvH at 2506.862 kb on + strand, within gcvH at 2506.862 kb on + strand, within gcvH at 2506.862 kb on + strand, within gcvH at 2506.862 kb on + strand, within gcvH at 2506.863 kb on - strand, within gcvH at 2506.872 kb on + strand, within gcvH at 2506.893 kb on + strand, within gcvH at 2507.015 kb on + strand at 2507.028 kb on + strand at 2507.028 kb on + strand at 2507.028 kb on + strand at 2507.048 kb on + strand at 2507.049 kb on - strand at 2507.049 kb on - strand at 2507.049 kb on - strand at 2507.172 kb on + strand at 2507.201 kb on - strand at 2507.306 kb on - strand at 2507.377 kb on + strand at 2507.377 kb on + strand at 2507.409 kb on - strand at 2507.483 kb on + strand, within gcvP at 2507.483 kb on + strand, within gcvP at 2507.492 kb on + strand, within gcvP at 2507.492 kb on + strand, within gcvP at 2507.493 kb on - strand, within gcvP at 2507.502 kb on - strand, within gcvP
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,504,660 - ubiI HEPCGN_18805 0.65 +0.8 2,504,930 + ubiI HEPCGN_18805 0.87 +1.8 2,505,106 + -1.4 2,505,175 + +0.3 2,505,176 - +0.5 2,505,276 + -0.7 2,505,555 + +0.6 2,505,556 - -0.4 2,505,621 + +0.6 2,505,622 - -1.4 2,505,622 - +0.4 2,505,657 - gcvT HEPCGN_18810 0.11 +0.2 2,505,686 + gcvT HEPCGN_18810 0.14 +1.1 2,505,686 + gcvT HEPCGN_18810 0.14 -0.3 2,505,686 + gcvT HEPCGN_18810 0.14 -0.3 2,505,686 + gcvT HEPCGN_18810 0.14 -0.9 2,505,686 + gcvT HEPCGN_18810 0.14 -0.1 2,505,686 + gcvT HEPCGN_18810 0.14 +1.8 2,505,687 - gcvT HEPCGN_18810 0.14 +0.8 2,505,687 - gcvT HEPCGN_18810 0.14 -0.6 2,505,687 - gcvT HEPCGN_18810 0.14 +0.5 2,505,736 - gcvT HEPCGN_18810 0.18 -0.1 2,505,820 + gcvT HEPCGN_18810 0.26 +2.1 2,505,821 - gcvT HEPCGN_18810 0.26 +1.6 2,505,837 + gcvT HEPCGN_18810 0.28 +0.8 2,505,837 + gcvT HEPCGN_18810 0.28 -0.9 2,505,841 - gcvT HEPCGN_18810 0.28 +1.2 2,505,846 - gcvT HEPCGN_18810 0.28 +0.1 2,506,014 - gcvT HEPCGN_18810 0.44 +0.4 2,506,099 - gcvT HEPCGN_18810 0.52 +0.4 2,506,100 + gcvT HEPCGN_18810 0.52 -0.3 2,506,100 + gcvT HEPCGN_18810 0.52 +0.9 2,506,240 - gcvT HEPCGN_18810 0.64 -0.3 2,506,500 + gcvT HEPCGN_18810 0.88 -0.2 2,506,500 + gcvT HEPCGN_18810 0.88 -0.3 2,506,500 + gcvT HEPCGN_18810 0.88 +1.1 2,506,501 - gcvT HEPCGN_18810 0.88 -0.4 2,506,533 - -0.2 2,506,613 + +0.5 2,506,662 + +1.8 2,506,680 - +0.3 2,506,724 + gcvH HEPCGN_18815 0.18 +0.3 2,506,725 - gcvH HEPCGN_18815 0.19 +0.2 2,506,843 - gcvH HEPCGN_18815 0.49 -0.8 2,506,862 + gcvH HEPCGN_18815 0.54 +0.8 2,506,862 + gcvH HEPCGN_18815 0.54 -0.4 2,506,862 + gcvH HEPCGN_18815 0.54 +2.2 2,506,862 + gcvH HEPCGN_18815 0.54 -1.5 2,506,863 - gcvH HEPCGN_18815 0.54 -0.1 2,506,872 + gcvH HEPCGN_18815 0.56 -0.3 2,506,893 + gcvH HEPCGN_18815 0.62 +0.6 2,507,015 + -0.4 2,507,028 + -0.5 2,507,028 + -0.1 2,507,028 + +0.1 2,507,048 + -1.1 2,507,049 - -0.1 2,507,049 - +0.1 2,507,049 - -0.2 2,507,172 + +0.5 2,507,201 - -0.4 2,507,306 - +0.4 2,507,377 + +0.4 2,507,377 + +0.7 2,507,409 - +0.8 2,507,483 + gcvP HEPCGN_18820 0.11 -0.5 2,507,483 + gcvP HEPCGN_18820 0.11 +0.0 2,507,492 + gcvP HEPCGN_18820 0.12 +0.2 2,507,492 + gcvP HEPCGN_18820 0.12 +0.2 2,507,493 - gcvP HEPCGN_18820 0.12 +0.2 2,507,502 - gcvP HEPCGN_18820 0.12 +0.6
Or see this region's nucleotide sequence