Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livJ and panM are separated by 187 nucleotides panM and livK are separated by 423 nucleotides livK and livH are separated by 47 nucleotides livH and livM overlap by 4 nucleotides
HEPCGN_15550: livJ - branched chain amino acid ABC transporter substrate-binding protein LivJ, at 1,872,397 to 1,873,500
livJ
HEPCGN_15555: panM - aspartate 1-decarboxylase autocleavage activator PanM, at 1,873,688 to 1,874,071
panM
HEPCGN_15560: livK - high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK, at 1,874,495 to 1,875,604
livK
HEPCGN_15565: livH - high-affinity branched-chain amino acid ABC transporter permease LivH, at 1,875,652 to 1,876,578
livH
HEPCGN_15570: livM - branched chain amino acid ABC transporter permease LivM, at 1,876,575 to 1,877,852
livM
Position (kb)
1874
1875
1876 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1873.538 kb on - strand at 1873.632 kb on - strand at 1873.953 kb on + strand, within panM at 1873.954 kb on - strand, within panM at 1874.181 kb on - strand at 1874.181 kb on - strand at 1874.193 kb on - strand at 1874.193 kb on - strand at 1874.405 kb on + strand at 1874.495 kb on - strand at 1874.558 kb on - strand at 1874.569 kb on + strand at 1874.572 kb on + strand at 1874.663 kb on - strand, within livK at 1874.709 kb on - strand, within livK at 1874.773 kb on + strand, within livK at 1874.773 kb on + strand, within livK at 1874.773 kb on + strand, within livK at 1874.777 kb on + strand, within livK at 1874.777 kb on + strand, within livK at 1874.824 kb on + strand, within livK at 1874.825 kb on - strand, within livK at 1874.828 kb on + strand, within livK at 1874.828 kb on + strand, within livK at 1874.828 kb on + strand, within livK at 1874.829 kb on - strand, within livK at 1874.842 kb on + strand, within livK at 1874.900 kb on + strand, within livK at 1874.901 kb on - strand, within livK at 1874.901 kb on - strand, within livK at 1874.901 kb on - strand, within livK at 1874.901 kb on - strand, within livK at 1874.912 kb on - strand, within livK at 1874.954 kb on + strand, within livK at 1874.955 kb on - strand, within livK at 1875.086 kb on - strand, within livK at 1875.389 kb on + strand, within livK at 1875.389 kb on + strand, within livK at 1875.389 kb on + strand, within livK at 1875.390 kb on - strand, within livK at 1875.390 kb on - strand, within livK at 1875.390 kb on - strand, within livK at 1875.500 kb on - strand at 1875.652 kb on - strand at 1875.670 kb on + strand at 1875.711 kb on + strand at 1875.737 kb on - strand at 1875.741 kb on + strand at 1875.741 kb on + strand at 1875.742 kb on - strand at 1875.790 kb on + strand, within livH at 1875.791 kb on - strand, within livH at 1875.791 kb on - strand, within livH at 1875.791 kb on - strand, within livH at 1875.791 kb on - strand, within livH at 1875.805 kb on + strand, within livH at 1875.805 kb on + strand, within livH at 1875.806 kb on - strand, within livH at 1875.806 kb on - strand, within livH at 1875.816 kb on + strand, within livH at 1875.991 kb on - strand, within livH at 1876.012 kb on + strand, within livH at 1876.012 kb on + strand, within livH at 1876.013 kb on - strand, within livH at 1876.110 kb on + strand, within livH at 1876.111 kb on - strand, within livH at 1876.141 kb on - strand, within livH at 1876.181 kb on - strand, within livH at 1876.337 kb on + strand, within livH at 1876.471 kb on + strand, within livH at 1876.471 kb on + strand, within livH at 1876.472 kb on - strand, within livH at 1876.486 kb on + strand at 1876.488 kb on + strand at 1876.489 kb on - strand at 1876.565 kb on + strand at 1876.574 kb on + strand at 1876.589 kb on + strand at 1876.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,873,538 - -0.8 1,873,632 - +0.5 1,873,953 + panM HEPCGN_15555 0.69 -0.3 1,873,954 - panM HEPCGN_15555 0.69 +1.9 1,874,181 - +0.4 1,874,181 - +0.7 1,874,193 - -0.8 1,874,193 - -0.5 1,874,405 + -0.2 1,874,495 - -0.1 1,874,558 - -0.1 1,874,569 + +0.5 1,874,572 + +0.4 1,874,663 - livK HEPCGN_15560 0.15 -0.9 1,874,709 - livK HEPCGN_15560 0.19 +0.0 1,874,773 + livK HEPCGN_15560 0.25 +1.9 1,874,773 + livK HEPCGN_15560 0.25 -1.1 1,874,773 + livK HEPCGN_15560 0.25 +1.2 1,874,777 + livK HEPCGN_15560 0.25 -0.0 1,874,777 + livK HEPCGN_15560 0.25 -0.4 1,874,824 + livK HEPCGN_15560 0.30 +0.5 1,874,825 - livK HEPCGN_15560 0.30 -0.2 1,874,828 + livK HEPCGN_15560 0.30 -0.1 1,874,828 + livK HEPCGN_15560 0.30 +0.4 1,874,828 + livK HEPCGN_15560 0.30 +1.1 1,874,829 - livK HEPCGN_15560 0.30 +1.5 1,874,842 + livK HEPCGN_15560 0.31 +0.0 1,874,900 + livK HEPCGN_15560 0.36 +0.6 1,874,901 - livK HEPCGN_15560 0.37 -0.6 1,874,901 - livK HEPCGN_15560 0.37 -0.9 1,874,901 - livK HEPCGN_15560 0.37 +0.3 1,874,901 - livK HEPCGN_15560 0.37 -1.7 1,874,912 - livK HEPCGN_15560 0.38 -0.1 1,874,954 + livK HEPCGN_15560 0.41 +0.6 1,874,955 - livK HEPCGN_15560 0.41 -1.5 1,875,086 - livK HEPCGN_15560 0.53 -0.1 1,875,389 + livK HEPCGN_15560 0.81 -0.5 1,875,389 + livK HEPCGN_15560 0.81 +1.6 1,875,389 + livK HEPCGN_15560 0.81 -2.4 1,875,390 - livK HEPCGN_15560 0.81 -0.1 1,875,390 - livK HEPCGN_15560 0.81 +0.2 1,875,390 - livK HEPCGN_15560 0.81 -0.5 1,875,500 - -0.7 1,875,652 - +0.1 1,875,670 + -0.3 1,875,711 + +0.5 1,875,737 - -0.2 1,875,741 + +0.6 1,875,741 + -0.9 1,875,742 - +0.3 1,875,790 + livH HEPCGN_15565 0.15 -0.5 1,875,791 - livH HEPCGN_15565 0.15 +0.4 1,875,791 - livH HEPCGN_15565 0.15 -0.1 1,875,791 - livH HEPCGN_15565 0.15 -0.3 1,875,791 - livH HEPCGN_15565 0.15 +0.1 1,875,805 + livH HEPCGN_15565 0.17 -0.5 1,875,805 + livH HEPCGN_15565 0.17 +0.5 1,875,806 - livH HEPCGN_15565 0.17 -1.1 1,875,806 - livH HEPCGN_15565 0.17 +2.0 1,875,816 + livH HEPCGN_15565 0.18 -0.9 1,875,991 - livH HEPCGN_15565 0.37 +0.4 1,876,012 + livH HEPCGN_15565 0.39 -1.6 1,876,012 + livH HEPCGN_15565 0.39 +0.3 1,876,013 - livH HEPCGN_15565 0.39 -0.2 1,876,110 + livH HEPCGN_15565 0.49 +0.2 1,876,111 - livH HEPCGN_15565 0.50 -0.1 1,876,141 - livH HEPCGN_15565 0.53 +0.0 1,876,181 - livH HEPCGN_15565 0.57 +1.3 1,876,337 + livH HEPCGN_15565 0.74 +0.8 1,876,471 + livH HEPCGN_15565 0.88 -0.5 1,876,471 + livH HEPCGN_15565 0.88 -0.2 1,876,472 - livH HEPCGN_15565 0.88 -0.3 1,876,486 + +4.2 1,876,488 + -2.1 1,876,489 - -0.1 1,876,565 + +0.5 1,876,574 + +0.3 1,876,589 + +0.5 1,876,590 - +0.7
Or see this region's nucleotide sequence