Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gyrB and tnp-IS3 are separated by 155 nucleotides tnp-IS3 and tnp-IS1397 are separated by 224 nucleotides tnp-IS1397 and yidB are separated by 170 nucleotides yidB and yidA are separated by 114 nucleotides
HEPCGN_13725: gyrB - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 1,495,099 to 1,497,513
gyrB
HEPCGN_13735: tnp-IS3 - IS3 family transposase, at 1,497,669 to 1,498,190
tnp-IS3
HEPCGN_13740: tnp-IS1397 - IS3 family IS1397 transposase, at 1,498,415 to 1,499,017
tnp-IS1397
HEPCGN_13745: yidB - Uncharacterized protein YidB, at 1,499,188 to 1,499,586
yidB
HEPCGN_13750: yidA - sugar-phosphatase, at 1,499,701 to 1,500,513
yidA
Position (kb)
1498
1499
1500 Strain fitness (log2 ratio)
-1
0
1 at 1497.512 kb on - strand at 1497.520 kb on + strand at 1497.520 kb on + strand at 1499.075 kb on + strand at 1499.076 kb on - strand at 1499.076 kb on - strand at 1499.076 kb on - strand at 1499.076 kb on - strand at 1499.077 kb on + strand at 1499.077 kb on + strand at 1499.078 kb on - strand at 1499.129 kb on + strand at 1499.130 kb on - strand at 1499.145 kb on + strand at 1499.146 kb on - strand at 1499.174 kb on + strand at 1499.185 kb on - strand at 1499.242 kb on + strand, within yidB at 1499.318 kb on + strand, within yidB at 1499.319 kb on - strand, within yidB at 1499.319 kb on - strand, within yidB at 1499.319 kb on - strand, within yidB at 1499.449 kb on - strand, within yidB at 1499.463 kb on - strand, within yidB at 1499.463 kb on - strand, within yidB at 1499.533 kb on - strand, within yidB at 1499.688 kb on - strand at 1499.688 kb on - strand at 1499.706 kb on + strand at 1499.858 kb on - strand, within yidA at 1499.858 kb on - strand, within yidA at 1499.875 kb on - strand, within yidA at 1499.893 kb on + strand, within yidA at 1499.894 kb on - strand, within yidA at 1499.901 kb on + strand, within yidA at 1499.902 kb on - strand, within yidA at 1499.902 kb on - strand, within yidA at 1499.902 kb on - strand, within yidA at 1499.905 kb on + strand, within yidA at 1499.905 kb on + strand, within yidA at 1499.906 kb on - strand, within yidA at 1499.921 kb on + strand, within yidA at 1499.940 kb on + strand, within yidA at 1499.968 kb on + strand, within yidA at 1499.968 kb on + strand, within yidA at 1499.969 kb on - strand, within yidA
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,497,512 - +1.1 1,497,520 + +0.2 1,497,520 + +0.3 1,499,075 + +0.1 1,499,076 - -0.0 1,499,076 - -0.7 1,499,076 - -0.8 1,499,076 - +0.1 1,499,077 + -0.0 1,499,077 + +0.9 1,499,078 - -1.2 1,499,129 + +0.1 1,499,130 - -0.4 1,499,145 + +0.3 1,499,146 - +0.3 1,499,174 + -0.9 1,499,185 - -1.3 1,499,242 + yidB HEPCGN_13745 0.14 -0.2 1,499,318 + yidB HEPCGN_13745 0.33 +1.0 1,499,319 - yidB HEPCGN_13745 0.33 -0.1 1,499,319 - yidB HEPCGN_13745 0.33 +0.4 1,499,319 - yidB HEPCGN_13745 0.33 -0.6 1,499,449 - yidB HEPCGN_13745 0.65 +0.5 1,499,463 - yidB HEPCGN_13745 0.69 +0.7 1,499,463 - yidB HEPCGN_13745 0.69 -0.7 1,499,533 - yidB HEPCGN_13745 0.86 +0.3 1,499,688 - +0.6 1,499,688 - +0.2 1,499,706 + +0.8 1,499,858 - yidA HEPCGN_13750 0.19 +0.8 1,499,858 - yidA HEPCGN_13750 0.19 -0.3 1,499,875 - yidA HEPCGN_13750 0.21 +0.5 1,499,893 + yidA HEPCGN_13750 0.24 +0.1 1,499,894 - yidA HEPCGN_13750 0.24 +0.5 1,499,901 + yidA HEPCGN_13750 0.25 -0.5 1,499,902 - yidA HEPCGN_13750 0.25 +0.2 1,499,902 - yidA HEPCGN_13750 0.25 -0.1 1,499,902 - yidA HEPCGN_13750 0.25 -0.2 1,499,905 + yidA HEPCGN_13750 0.25 +0.8 1,499,905 + yidA HEPCGN_13750 0.25 +0.0 1,499,906 - yidA HEPCGN_13750 0.25 -0.4 1,499,921 + yidA HEPCGN_13750 0.27 -0.2 1,499,940 + yidA HEPCGN_13750 0.29 +1.5 1,499,968 + yidA HEPCGN_13750 0.33 +0.1 1,499,968 + yidA HEPCGN_13750 0.33 +0.1 1,499,969 - yidA HEPCGN_13750 0.33 -1.3
Or see this region's nucleotide sequence