Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgK and ydgT are separated by 85 nucleotides ydgT and ydgJ are separated by 640 nucleotides ydgJ and add are separated by 35 nucleotides
HEPCGN_05945: ydgK - Inner membrane protein YdgK, at 1,059,601 to 1,060,041
ydgK
HEPCGN_05950: ydgT - transcription modulator YdgT, at 1,060,127 to 1,060,342
ydgT
HEPCGN_05955: ydgJ - Uncharacterized oxidoreductase YdgJ, at 1,060,983 to 1,062,023
ydgJ
HEPCGN_05960: add - adenosine deaminase, at 1,062,059 to 1,063,060
add
Position (kb)
1060
1061
1062
1063 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1060.041 kb on + strand at 1060.042 kb on - strand at 1060.084 kb on - strand at 1060.095 kb on + strand at 1060.095 kb on + strand at 1060.097 kb on + strand at 1060.106 kb on + strand at 1060.107 kb on - strand at 1060.225 kb on - strand, within ydgT at 1060.229 kb on - strand, within ydgT at 1060.342 kb on + strand at 1060.655 kb on + strand at 1060.656 kb on - strand at 1060.796 kb on - strand at 1060.805 kb on - strand at 1060.961 kb on + strand at 1060.961 kb on + strand at 1060.962 kb on - strand at 1061.176 kb on + strand, within ydgJ at 1061.176 kb on + strand, within ydgJ at 1061.195 kb on + strand, within ydgJ at 1061.196 kb on - strand, within ydgJ at 1061.404 kb on + strand, within ydgJ at 1061.405 kb on - strand, within ydgJ at 1061.405 kb on - strand, within ydgJ at 1061.421 kb on + strand, within ydgJ at 1061.421 kb on + strand, within ydgJ at 1061.540 kb on + strand, within ydgJ at 1061.587 kb on + strand, within ydgJ at 1061.587 kb on + strand, within ydgJ at 1061.588 kb on - strand, within ydgJ at 1061.694 kb on + strand, within ydgJ at 1061.695 kb on - strand, within ydgJ at 1061.695 kb on - strand, within ydgJ at 1061.796 kb on + strand, within ydgJ at 1061.816 kb on - strand, within ydgJ at 1061.889 kb on + strand, within ydgJ at 1061.890 kb on - strand, within ydgJ at 1061.912 kb on - strand, within ydgJ at 1061.959 kb on + strand at 1061.959 kb on + strand at 1062.042 kb on - strand at 1062.130 kb on + strand at 1062.190 kb on - strand, within add at 1062.236 kb on - strand, within add at 1062.481 kb on + strand, within add at 1062.482 kb on - strand, within add at 1062.967 kb on + strand at 1062.967 kb on + strand at 1062.968 kb on - strand at 1062.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,060,041 + +0.0 1,060,042 - -0.4 1,060,084 - -0.7 1,060,095 + +0.3 1,060,095 + +0.9 1,060,097 + +0.5 1,060,106 + -0.1 1,060,107 - +1.2 1,060,225 - ydgT HEPCGN_05950 0.45 -1.2 1,060,229 - ydgT HEPCGN_05950 0.47 +0.9 1,060,342 + +1.3 1,060,655 + -0.4 1,060,656 - +0.4 1,060,796 - +0.6 1,060,805 - +0.4 1,060,961 + +2.2 1,060,961 + -0.2 1,060,962 - +0.7 1,061,176 + ydgJ HEPCGN_05955 0.19 +2.9 1,061,176 + ydgJ HEPCGN_05955 0.19 -2.0 1,061,195 + ydgJ HEPCGN_05955 0.20 -0.3 1,061,196 - ydgJ HEPCGN_05955 0.20 +1.3 1,061,404 + ydgJ HEPCGN_05955 0.40 +0.5 1,061,405 - ydgJ HEPCGN_05955 0.41 +0.3 1,061,405 - ydgJ HEPCGN_05955 0.41 +0.3 1,061,421 + ydgJ HEPCGN_05955 0.42 -0.5 1,061,421 + ydgJ HEPCGN_05955 0.42 +0.4 1,061,540 + ydgJ HEPCGN_05955 0.54 +1.1 1,061,587 + ydgJ HEPCGN_05955 0.58 -0.2 1,061,587 + ydgJ HEPCGN_05955 0.58 +0.8 1,061,588 - ydgJ HEPCGN_05955 0.58 +0.7 1,061,694 + ydgJ HEPCGN_05955 0.68 +0.4 1,061,695 - ydgJ HEPCGN_05955 0.68 +0.9 1,061,695 - ydgJ HEPCGN_05955 0.68 +0.5 1,061,796 + ydgJ HEPCGN_05955 0.78 +1.6 1,061,816 - ydgJ HEPCGN_05955 0.80 +0.2 1,061,889 + ydgJ HEPCGN_05955 0.87 -1.5 1,061,890 - ydgJ HEPCGN_05955 0.87 +1.6 1,061,912 - ydgJ HEPCGN_05955 0.89 +0.3 1,061,959 + -1.3 1,061,959 + -0.7 1,062,042 - +0.1 1,062,130 + +1.3 1,062,190 - add HEPCGN_05960 0.13 -0.0 1,062,236 - add HEPCGN_05960 0.18 -0.3 1,062,481 + add HEPCGN_05960 0.42 +1.3 1,062,482 - add HEPCGN_05960 0.42 +1.3 1,062,967 + +0.5 1,062,967 + -1.5 1,062,968 - -0.7 1,062,969 + +0.9
Or see this region's nucleotide sequence