Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaE and wcaF are separated by 15 nucleotides wcaF and HEPCGN_03045 are separated by 26 nucleotides HEPCGN_03045 and fcl are separated by 2 nucleotides fcl and gmm overlap by 1 nucleotides
HEPCGN_03035: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 516,186 to 516,932
wcaE
HEPCGN_03040: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 516,948 to 517,496
wcaF
HEPCGN_03045: HEPCGN_03045 - GDP-mannose 4,6-dehydratase, at 517,523 to 518,644
_03045
HEPCGN_03050: fcl - GDP-L-fucose synthase, at 518,647 to 519,612
fcl
HEPCGN_03055: gmm - GDP-mannose mannosyl hydrolase, at 519,612 to 520,094
gmm
Position (kb) 517
518
519 Strain fitness (log2 ratio) -1
0
1
2 at 516.600 kb on - strand, within wcaE at 517.039 kb on + strand, within wcaF at 517.204 kb on + strand, within wcaF at 517.223 kb on - strand, within wcaF at 517.264 kb on + strand, within wcaF at 517.571 kb on + strand at 517.571 kb on + strand at 517.615 kb on + strand at 517.615 kb on + strand at 517.616 kb on - strand at 517.619 kb on - strand at 517.724 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.823 kb on + strand, within HEPCGN_03045 at 517.907 kb on + strand, within HEPCGN_03045 at 518.010 kb on - strand, within HEPCGN_03045 at 518.010 kb on - strand, within HEPCGN_03045 at 518.016 kb on - strand, within HEPCGN_03045 at 518.029 kb on + strand, within HEPCGN_03045 at 518.055 kb on - strand, within HEPCGN_03045 at 518.292 kb on - strand, within HEPCGN_03045 at 518.652 kb on + strand at 518.667 kb on + strand at 518.741 kb on + strand at 518.741 kb on + strand at 518.741 kb on + strand at 518.745 kb on - strand, within fcl at 518.745 kb on - strand, within fcl at 518.745 kb on - strand, within fcl at 518.812 kb on - strand, within fcl at 519.174 kb on + strand, within fcl at 519.483 kb on + strand, within fcl at 519.571 kb on + strand at 519.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 516,600 - wcaE HEPCGN_03035 0.55 -1.3 517,039 + wcaF HEPCGN_03040 0.17 +0.6 517,204 + wcaF HEPCGN_03040 0.47 -0.1 517,223 - wcaF HEPCGN_03040 0.50 +0.2 517,264 + wcaF HEPCGN_03040 0.58 -0.8 517,571 + +0.1 517,571 + +0.3 517,615 + -1.5 517,615 + +0.4 517,616 - -0.1 517,619 - +1.8 517,724 - HEPCGN_03045 0.18 -0.2 517,773 - HEPCGN_03045 0.22 -0.6 517,773 - HEPCGN_03045 0.22 -0.8 517,773 - HEPCGN_03045 0.22 +2.1 517,823 + HEPCGN_03045 0.27 -0.8 517,907 + HEPCGN_03045 0.34 -0.1 518,010 - HEPCGN_03045 0.43 +1.2 518,010 - HEPCGN_03045 0.43 +2.0 518,016 - HEPCGN_03045 0.44 -0.1 518,029 + HEPCGN_03045 0.45 -1.0 518,055 - HEPCGN_03045 0.47 +0.0 518,292 - HEPCGN_03045 0.69 +0.2 518,652 + -1.6 518,667 + -0.0 518,741 + -0.1 518,741 + +0.5 518,741 + +0.7 518,745 - fcl HEPCGN_03050 0.10 +0.1 518,745 - fcl HEPCGN_03050 0.10 +0.1 518,745 - fcl HEPCGN_03050 0.10 +1.5 518,812 - fcl HEPCGN_03050 0.17 +0.3 519,174 + fcl HEPCGN_03050 0.55 -0.4 519,483 + fcl HEPCGN_03050 0.87 +1.1 519,571 + +1.4 519,610 + +0.1
Or see this region's nucleotide sequence