Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fiu and mcbA are separated by 264 nucleotides mcbA and rlmF are separated by 275 nucleotides rlmF and ybiO overlap by 4 nucleotides
HEPCGN_01800: fiu - catecholate siderophore receptor Fiu, at 282,921 to 285,203
fiu
HEPCGN_01805: mcbA - DUF1471 domain-containing protein, at 285,468 to 285,728
mcbA
HEPCGN_01810: rlmF - 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF, at 286,004 to 286,930
rlmF
HEPCGN_01815: ybiO - mechanosensitive channel protein, at 286,927 to 289,152
ybiO
Position (kb) 286
287 Strain fitness (log2 ratio) -1
0
1
2 at 285.137 kb on + strand at 285.137 kb on + strand at 285.224 kb on - strand at 285.225 kb on + strand at 285.225 kb on + strand at 285.225 kb on + strand at 285.225 kb on + strand at 285.226 kb on - strand at 285.226 kb on - strand at 285.226 kb on - strand at 285.226 kb on - strand at 285.385 kb on - strand at 285.469 kb on + strand at 285.476 kb on - strand at 285.489 kb on - strand at 285.521 kb on + strand, within mcbA at 285.521 kb on + strand, within mcbA at 285.529 kb on + strand, within mcbA at 285.529 kb on + strand, within mcbA at 285.586 kb on - strand, within mcbA at 285.586 kb on - strand, within mcbA at 285.648 kb on - strand, within mcbA at 285.667 kb on + strand, within mcbA at 285.728 kb on + strand at 285.747 kb on - strand at 285.747 kb on - strand at 285.772 kb on - strand at 285.872 kb on + strand at 285.948 kb on + strand at 285.948 kb on + strand at 285.949 kb on - strand at 285.949 kb on - strand at 285.978 kb on + strand at 285.979 kb on - strand at 286.003 kb on + strand at 286.135 kb on - strand, within rlmF at 286.259 kb on + strand, within rlmF at 286.268 kb on + strand, within rlmF at 286.351 kb on + strand, within rlmF at 286.351 kb on + strand, within rlmF at 286.379 kb on + strand, within rlmF at 286.380 kb on - strand, within rlmF at 286.393 kb on + strand, within rlmF at 286.394 kb on - strand, within rlmF at 286.538 kb on + strand, within rlmF at 286.616 kb on - strand, within rlmF at 286.794 kb on - strand, within rlmF at 286.871 kb on - strand at 286.994 kb on + strand at 286.995 kb on - strand at 287.113 kb on - strand at 287.298 kb on + strand, within ybiO at 287.299 kb on - strand, within ybiO at 287.307 kb on + strand, within ybiO at 287.307 kb on + strand, within ybiO at 287.308 kb on - strand, within ybiO at 287.308 kb on - strand, within ybiO at 287.336 kb on + strand, within ybiO at 287.451 kb on + strand, within ybiO at 287.451 kb on + strand, within ybiO at 287.451 kb on + strand, within ybiO at 287.452 kb on - strand, within ybiO at 287.512 kb on + strand, within ybiO at 287.611 kb on - strand, within ybiO at 287.758 kb on - strand, within ybiO at 287.761 kb on + strand, within ybiO at 287.762 kb on - strand, within ybiO at 287.836 kb on + strand, within ybiO
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 285,137 + +0.7 285,137 + +0.7 285,224 - -0.2 285,225 + +0.2 285,225 + -0.4 285,225 + +0.6 285,225 + -0.8 285,226 - +0.1 285,226 - -0.4 285,226 - +0.5 285,226 - +0.1 285,385 - -0.4 285,469 + +0.1 285,476 - -0.1 285,489 - +0.4 285,521 + mcbA HEPCGN_01805 0.20 +0.5 285,521 + mcbA HEPCGN_01805 0.20 -0.8 285,529 + mcbA HEPCGN_01805 0.23 -0.3 285,529 + mcbA HEPCGN_01805 0.23 +0.9 285,586 - mcbA HEPCGN_01805 0.45 -1.1 285,586 - mcbA HEPCGN_01805 0.45 -0.6 285,648 - mcbA HEPCGN_01805 0.69 -0.5 285,667 + mcbA HEPCGN_01805 0.76 -0.3 285,728 + -0.2 285,747 - +0.5 285,747 - +0.8 285,772 - -1.1 285,872 + -0.2 285,948 + +0.1 285,948 + -0.3 285,949 - +0.9 285,949 - +0.4 285,978 + -0.5 285,979 - +0.3 286,003 + +0.1 286,135 - rlmF HEPCGN_01810 0.14 +1.8 286,259 + rlmF HEPCGN_01810 0.28 -0.1 286,268 + rlmF HEPCGN_01810 0.28 +0.6 286,351 + rlmF HEPCGN_01810 0.37 +1.2 286,351 + rlmF HEPCGN_01810 0.37 -0.1 286,379 + rlmF HEPCGN_01810 0.40 -0.5 286,380 - rlmF HEPCGN_01810 0.41 -0.1 286,393 + rlmF HEPCGN_01810 0.42 -1.2 286,394 - rlmF HEPCGN_01810 0.42 -0.1 286,538 + rlmF HEPCGN_01810 0.58 -0.2 286,616 - rlmF HEPCGN_01810 0.66 +0.8 286,794 - rlmF HEPCGN_01810 0.85 -0.6 286,871 - -1.1 286,994 + -0.0 286,995 - +0.3 287,113 - +0.0 287,298 + ybiO HEPCGN_01815 0.17 +0.1 287,299 - ybiO HEPCGN_01815 0.17 +0.6 287,307 + ybiO HEPCGN_01815 0.17 +1.1 287,307 + ybiO HEPCGN_01815 0.17 -0.0 287,308 - ybiO HEPCGN_01815 0.17 -0.5 287,308 - ybiO HEPCGN_01815 0.17 +0.9 287,336 + ybiO HEPCGN_01815 0.18 +0.5 287,451 + ybiO HEPCGN_01815 0.24 +0.9 287,451 + ybiO HEPCGN_01815 0.24 +0.0 287,451 + ybiO HEPCGN_01815 0.24 -0.3 287,452 - ybiO HEPCGN_01815 0.24 +0.4 287,512 + ybiO HEPCGN_01815 0.26 -0.2 287,611 - ybiO HEPCGN_01815 0.31 -0.3 287,758 - ybiO HEPCGN_01815 0.37 +0.5 287,761 + ybiO HEPCGN_01815 0.37 +0.7 287,762 - ybiO HEPCGN_01815 0.38 +0.2 287,836 + ybiO HEPCGN_01815 0.41 +0.4
Or see this region's nucleotide sequence