Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt curA and mcbR are separated by 198 nucleotides mcbR and yncD are separated by 35 nucleotides yncD and yncE are separated by 110 nucleotides
HEPCGN_26280: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 4,000,585 to 4,001,622
curA
HEPCGN_26285: mcbR - colanic acid/biofilm transcriptional regulator McbR, at 4,001,821 to 4,002,486
mcbR
HEPCGN_26290: yncD - putative TonB-dependent receptor YncD, at 4,002,522 to 4,004,756
yncD
HEPCGN_26295: yncE - Uncharacterized protein YncE, at 4,004,867 to 4,005,928
yncE
Position (kb)
4002
4003
4004
4005 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4001.559 kb on + strand at 4001.744 kb on + strand at 4001.817 kb on + strand at 4001.860 kb on - strand at 4001.893 kb on - strand, within mcbR at 4001.964 kb on + strand, within mcbR at 4002.036 kb on + strand, within mcbR at 4002.374 kb on - strand, within mcbR at 4002.478 kb on - strand at 4002.490 kb on - strand at 4002.692 kb on + strand at 4002.720 kb on - strand at 4002.770 kb on + strand, within yncD at 4002.773 kb on - strand, within yncD at 4002.815 kb on - strand, within yncD at 4002.815 kb on - strand, within yncD at 4002.837 kb on - strand, within yncD at 4002.969 kb on + strand, within yncD at 4003.028 kb on + strand, within yncD at 4003.184 kb on - strand, within yncD at 4003.239 kb on - strand, within yncD at 4003.239 kb on - strand, within yncD at 4003.295 kb on - strand, within yncD at 4003.332 kb on - strand, within yncD at 4003.337 kb on - strand, within yncD at 4003.337 kb on - strand, within yncD at 4003.369 kb on + strand, within yncD at 4003.423 kb on - strand, within yncD at 4003.670 kb on + strand, within yncD at 4003.726 kb on + strand, within yncD at 4003.726 kb on + strand, within yncD at 4003.742 kb on + strand, within yncD at 4003.742 kb on + strand, within yncD at 4003.743 kb on - strand, within yncD at 4003.772 kb on - strand, within yncD at 4003.920 kb on + strand, within yncD at 4004.032 kb on + strand, within yncD at 4004.116 kb on + strand, within yncD at 4004.195 kb on - strand, within yncD at 4004.397 kb on + strand, within yncD at 4004.439 kb on - strand, within yncD at 4004.439 kb on - strand, within yncD at 4004.713 kb on + strand at 4004.714 kb on - strand at 4004.786 kb on + strand at 4004.810 kb on - strand at 4004.874 kb on + strand at 4004.910 kb on + strand at 4004.987 kb on - strand, within yncE at 4005.011 kb on - strand, within yncE at 4005.011 kb on - strand, within yncE at 4005.213 kb on + strand, within yncE at 4005.306 kb on + strand, within yncE at 4005.465 kb on - strand, within yncE at 4005.616 kb on + strand, within yncE at 4005.634 kb on - strand, within yncE at 4005.748 kb on + strand, within yncE
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 4,001,559 + -1.2 4,001,744 + +0.4 4,001,817 + -0.6 4,001,860 - -0.1 4,001,893 - mcbR HEPCGN_26285 0.11 +0.9 4,001,964 + mcbR HEPCGN_26285 0.21 +0.9 4,002,036 + mcbR HEPCGN_26285 0.32 -2.3 4,002,374 - mcbR HEPCGN_26285 0.83 +0.3 4,002,478 - +0.3 4,002,490 - -0.6 4,002,692 + +0.1 4,002,720 - -1.0 4,002,770 + yncD HEPCGN_26290 0.11 +0.3 4,002,773 - yncD HEPCGN_26290 0.11 +0.9 4,002,815 - yncD HEPCGN_26290 0.13 -0.3 4,002,815 - yncD HEPCGN_26290 0.13 -1.1 4,002,837 - yncD HEPCGN_26290 0.14 +0.4 4,002,969 + yncD HEPCGN_26290 0.20 +0.1 4,003,028 + yncD HEPCGN_26290 0.23 +0.6 4,003,184 - yncD HEPCGN_26290 0.30 -0.7 4,003,239 - yncD HEPCGN_26290 0.32 +0.6 4,003,239 - yncD HEPCGN_26290 0.32 +0.5 4,003,295 - yncD HEPCGN_26290 0.35 +0.4 4,003,332 - yncD HEPCGN_26290 0.36 +2.4 4,003,337 - yncD HEPCGN_26290 0.36 -1.1 4,003,337 - yncD HEPCGN_26290 0.36 -2.4 4,003,369 + yncD HEPCGN_26290 0.38 -0.7 4,003,423 - yncD HEPCGN_26290 0.40 -1.0 4,003,670 + yncD HEPCGN_26290 0.51 -0.8 4,003,726 + yncD HEPCGN_26290 0.54 +0.2 4,003,726 + yncD HEPCGN_26290 0.54 -0.5 4,003,742 + yncD HEPCGN_26290 0.55 -0.5 4,003,742 + yncD HEPCGN_26290 0.55 -0.7 4,003,743 - yncD HEPCGN_26290 0.55 +1.4 4,003,772 - yncD HEPCGN_26290 0.56 -0.0 4,003,920 + yncD HEPCGN_26290 0.63 -0.1 4,004,032 + yncD HEPCGN_26290 0.68 +0.9 4,004,116 + yncD HEPCGN_26290 0.71 +0.5 4,004,195 - yncD HEPCGN_26290 0.75 +2.2 4,004,397 + yncD HEPCGN_26290 0.84 +0.9 4,004,439 - yncD HEPCGN_26290 0.86 +0.1 4,004,439 - yncD HEPCGN_26290 0.86 -0.1 4,004,713 + +0.3 4,004,714 - +0.7 4,004,786 + +0.2 4,004,810 - -2.6 4,004,874 + -0.6 4,004,910 + +0.4 4,004,987 - yncE HEPCGN_26295 0.11 -1.9 4,005,011 - yncE HEPCGN_26295 0.14 +0.4 4,005,011 - yncE HEPCGN_26295 0.14 -0.5 4,005,213 + yncE HEPCGN_26295 0.33 +1.5 4,005,306 + yncE HEPCGN_26295 0.41 +0.5 4,005,465 - yncE HEPCGN_26295 0.56 -0.5 4,005,616 + yncE HEPCGN_26295 0.71 +0.3 4,005,634 - yncE HEPCGN_26295 0.72 -1.6 4,005,748 + yncE HEPCGN_26295 0.83 +0.1
Or see this region's nucleotide sequence