Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt curA and mcbR are separated by 198 nucleotides mcbR and yncD are separated by 35 nucleotides
HEPCGN_26280: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 4,000,585 to 4,001,622
curA
HEPCGN_26285: mcbR - colanic acid/biofilm transcriptional regulator McbR, at 4,001,821 to 4,002,486
mcbR
HEPCGN_26290: yncD - putative TonB-dependent receptor YncD, at 4,002,522 to 4,004,756
yncD
Position (kb) 4001
4002
4003 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4000.897 kb on + strand, within curA at 4000.904 kb on + strand, within curA at 4000.905 kb on - strand, within curA at 4000.996 kb on + strand, within curA at 4000.996 kb on + strand, within curA at 4000.997 kb on - strand, within curA at 4000.998 kb on + strand, within curA at 4001.048 kb on + strand, within curA at 4001.238 kb on + strand, within curA at 4001.245 kb on - strand, within curA at 4001.248 kb on + strand, within curA at 4001.249 kb on - strand, within curA at 4001.273 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.302 kb on - strand, within curA at 4001.302 kb on - strand, within curA at 4001.342 kb on - strand, within curA at 4001.559 kb on + strand at 4001.744 kb on + strand at 4001.817 kb on + strand at 4001.860 kb on - strand at 4001.893 kb on - strand, within mcbR at 4001.964 kb on + strand, within mcbR at 4002.036 kb on + strand, within mcbR at 4002.374 kb on - strand, within mcbR at 4002.478 kb on - strand at 4002.490 kb on - strand at 4002.692 kb on + strand at 4002.720 kb on - strand at 4002.770 kb on + strand, within yncD at 4002.773 kb on - strand, within yncD at 4002.815 kb on - strand, within yncD at 4002.815 kb on - strand, within yncD at 4002.837 kb on - strand, within yncD at 4002.969 kb on + strand, within yncD at 4003.028 kb on + strand, within yncD at 4003.184 kb on - strand, within yncD at 4003.239 kb on - strand, within yncD at 4003.239 kb on - strand, within yncD at 4003.295 kb on - strand, within yncD at 4003.332 kb on - strand, within yncD at 4003.337 kb on - strand, within yncD at 4003.337 kb on - strand, within yncD at 4003.369 kb on + strand, within yncD at 4003.423 kb on - strand, within yncD
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 4,000,897 + curA HEPCGN_26280 0.30 +1.1 4,000,904 + curA HEPCGN_26280 0.31 +0.8 4,000,905 - curA HEPCGN_26280 0.31 +1.3 4,000,996 + curA HEPCGN_26280 0.40 +2.8 4,000,996 + curA HEPCGN_26280 0.40 -0.5 4,000,997 - curA HEPCGN_26280 0.40 +0.5 4,000,998 + curA HEPCGN_26280 0.40 -0.7 4,001,048 + curA HEPCGN_26280 0.45 -0.2 4,001,238 + curA HEPCGN_26280 0.63 +0.0 4,001,245 - curA HEPCGN_26280 0.64 -1.7 4,001,248 + curA HEPCGN_26280 0.64 -0.0 4,001,249 - curA HEPCGN_26280 0.64 -0.9 4,001,273 + curA HEPCGN_26280 0.66 -0.3 4,001,301 + curA HEPCGN_26280 0.69 +1.1 4,001,301 + curA HEPCGN_26280 0.69 +0.5 4,001,301 + curA HEPCGN_26280 0.69 -1.4 4,001,302 - curA HEPCGN_26280 0.69 -0.5 4,001,302 - curA HEPCGN_26280 0.69 +1.0 4,001,342 - curA HEPCGN_26280 0.73 -1.0 4,001,559 + -1.2 4,001,744 + +0.4 4,001,817 + -0.6 4,001,860 - -0.1 4,001,893 - mcbR HEPCGN_26285 0.11 +0.9 4,001,964 + mcbR HEPCGN_26285 0.21 +0.9 4,002,036 + mcbR HEPCGN_26285 0.32 -2.3 4,002,374 - mcbR HEPCGN_26285 0.83 +0.3 4,002,478 - +0.3 4,002,490 - -0.6 4,002,692 + +0.1 4,002,720 - -1.0 4,002,770 + yncD HEPCGN_26290 0.11 +0.3 4,002,773 - yncD HEPCGN_26290 0.11 +0.9 4,002,815 - yncD HEPCGN_26290 0.13 -0.3 4,002,815 - yncD HEPCGN_26290 0.13 -1.1 4,002,837 - yncD HEPCGN_26290 0.14 +0.4 4,002,969 + yncD HEPCGN_26290 0.20 +0.1 4,003,028 + yncD HEPCGN_26290 0.23 +0.6 4,003,184 - yncD HEPCGN_26290 0.30 -0.7 4,003,239 - yncD HEPCGN_26290 0.32 +0.6 4,003,239 - yncD HEPCGN_26290 0.32 +0.5 4,003,295 - yncD HEPCGN_26290 0.35 +0.4 4,003,332 - yncD HEPCGN_26290 0.36 +2.4 4,003,337 - yncD HEPCGN_26290 0.36 -1.1 4,003,337 - yncD HEPCGN_26290 0.36 -2.4 4,003,369 + yncD HEPCGN_26290 0.38 -0.7 4,003,423 - yncD HEPCGN_26290 0.40 -1.0
Or see this region's nucleotide sequence