Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefB and yheV are separated by 9 nucleotides yheV and slyD are separated by 94 nucleotides slyD and slyX are separated by 49 nucleotides slyX and fkpA are separated by 220 nucleotides
HEPCGN_16105: kefB - glutathione-regulated potassium-efflux system protein KefB, at 1,994,235 to 1,994,978
kefB
HEPCGN_16110: yheV - Uncharacterized protein YheV, at 1,994,988 to 1,995,188
yheV
HEPCGN_16115: slyD - peptidylprolyl isomerase, at 1,995,283 to 1,995,873
slyD
HEPCGN_16120: slyX - Protein SlyX, at 1,995,923 to 1,996,141
slyX
HEPCGN_16125: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase, at 1,996,362 to 1,997,174
fkpA
Position (kb)
1995
1996 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1994.299 kb on + strand at 1994.300 kb on - strand at 1994.300 kb on - strand at 1994.303 kb on + strand at 1994.369 kb on + strand, within kefB at 1994.370 kb on - strand, within kefB at 1994.439 kb on - strand, within kefB at 1994.439 kb on - strand, within kefB at 1994.497 kb on - strand, within kefB at 1994.543 kb on - strand, within kefB at 1994.623 kb on + strand, within kefB at 1994.623 kb on + strand, within kefB at 1994.623 kb on + strand, within kefB at 1994.624 kb on - strand, within kefB at 1994.651 kb on + strand, within kefB at 1994.692 kb on + strand, within kefB at 1994.695 kb on + strand, within kefB at 1994.695 kb on + strand, within kefB at 1994.696 kb on - strand, within kefB at 1994.743 kb on + strand, within kefB at 1995.008 kb on + strand, within yheV at 1995.009 kb on - strand, within yheV at 1995.071 kb on + strand, within yheV at 1995.072 kb on - strand, within yheV at 1995.074 kb on + strand, within yheV at 1995.075 kb on - strand, within yheV at 1995.080 kb on + strand, within yheV at 1995.096 kb on - strand, within yheV at 1995.209 kb on + strand at 1995.214 kb on + strand at 1995.214 kb on + strand at 1995.214 kb on + strand at 1995.214 kb on + strand at 1995.215 kb on - strand at 1995.215 kb on - strand at 1995.257 kb on + strand at 1995.279 kb on + strand at 1995.319 kb on + strand at 1995.320 kb on - strand at 1995.327 kb on - strand at 1995.330 kb on + strand at 1995.330 kb on + strand at 1995.331 kb on - strand at 1995.331 kb on - strand at 1995.484 kb on + strand, within slyD at 1995.522 kb on + strand, within slyD at 1995.523 kb on - strand, within slyD at 1995.530 kb on + strand, within slyD at 1995.535 kb on - strand, within slyD at 1995.557 kb on + strand, within slyD at 1995.558 kb on - strand, within slyD at 1995.558 kb on - strand, within slyD at 1995.632 kb on + strand, within slyD at 1995.632 kb on + strand, within slyD at 1995.632 kb on + strand, within slyD at 1995.646 kb on - strand, within slyD at 1995.850 kb on - strand at 1995.871 kb on + strand at 1995.872 kb on - strand at 1995.928 kb on - strand at 1995.986 kb on + strand, within slyX at 1995.987 kb on - strand, within slyX at 1996.130 kb on + strand at 1996.232 kb on + strand at 1996.253 kb on + strand at 1996.253 kb on + strand at 1996.254 kb on - strand at 1996.260 kb on + strand at 1996.260 kb on + strand at 1996.260 kb on + strand at 1996.261 kb on - strand at 1996.261 kb on - strand at 1996.261 kb on - strand at 1996.261 kb on - strand at 1996.263 kb on + strand at 1996.263 kb on + strand at 1996.264 kb on - strand at 1996.264 kb on - strand at 1996.276 kb on + strand at 1996.277 kb on - strand at 1996.277 kb on - strand at 1996.301 kb on + strand at 1996.301 kb on + strand at 1996.302 kb on - strand at 1996.303 kb on + strand at 1996.306 kb on + strand at 1996.306 kb on + strand at 1996.320 kb on + strand at 1996.320 kb on + strand at 1996.321 kb on - strand at 1996.321 kb on - strand at 1996.321 kb on - strand at 1996.321 kb on - strand at 1996.324 kb on + strand at 1996.325 kb on - strand at 1996.358 kb on - strand at 1996.376 kb on + strand at 1996.381 kb on + strand at 1996.536 kb on + strand, within fkpA at 1996.536 kb on + strand, within fkpA at 1996.536 kb on + strand, within fkpA at 1996.536 kb on + strand, within fkpA at 1996.536 kb on + strand, within fkpA at 1996.536 kb on + strand, within fkpA at 1996.537 kb on - strand, within fkpA at 1996.537 kb on - strand, within fkpA at 1996.819 kb on + strand, within fkpA at 1996.820 kb on - strand, within fkpA at 1996.820 kb on - strand, within fkpA at 1996.856 kb on + strand, within fkpA at 1996.856 kb on + strand, within fkpA
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 1,994,299 + +0.3 1,994,300 - -0.6 1,994,300 - +0.8 1,994,303 + +0.5 1,994,369 + kefB HEPCGN_16105 0.18 -1.6 1,994,370 - kefB HEPCGN_16105 0.18 -0.6 1,994,439 - kefB HEPCGN_16105 0.27 -0.7 1,994,439 - kefB HEPCGN_16105 0.27 -0.6 1,994,497 - kefB HEPCGN_16105 0.35 -0.1 1,994,543 - kefB HEPCGN_16105 0.41 +0.1 1,994,623 + kefB HEPCGN_16105 0.52 -0.3 1,994,623 + kefB HEPCGN_16105 0.52 -0.5 1,994,623 + kefB HEPCGN_16105 0.52 +0.6 1,994,624 - kefB HEPCGN_16105 0.52 +0.7 1,994,651 + kefB HEPCGN_16105 0.56 +0.7 1,994,692 + kefB HEPCGN_16105 0.61 -2.3 1,994,695 + kefB HEPCGN_16105 0.62 -1.1 1,994,695 + kefB HEPCGN_16105 0.62 -0.5 1,994,696 - kefB HEPCGN_16105 0.62 +0.8 1,994,743 + kefB HEPCGN_16105 0.68 -0.1 1,995,008 + yheV HEPCGN_16110 0.10 +0.1 1,995,009 - yheV HEPCGN_16110 0.10 -2.6 1,995,071 + yheV HEPCGN_16110 0.41 +0.0 1,995,072 - yheV HEPCGN_16110 0.42 +0.1 1,995,074 + yheV HEPCGN_16110 0.43 +0.1 1,995,075 - yheV HEPCGN_16110 0.43 -1.1 1,995,080 + yheV HEPCGN_16110 0.46 -2.4 1,995,096 - yheV HEPCGN_16110 0.54 +0.3 1,995,209 + -0.1 1,995,214 + -0.5 1,995,214 + -0.3 1,995,214 + -1.9 1,995,214 + -0.7 1,995,215 - -0.6 1,995,215 - -0.5 1,995,257 + +0.5 1,995,279 + -0.4 1,995,319 + +0.2 1,995,320 - +0.0 1,995,327 - -0.6 1,995,330 + -1.1 1,995,330 + -0.1 1,995,331 - +0.2 1,995,331 - -0.3 1,995,484 + slyD HEPCGN_16115 0.34 -1.8 1,995,522 + slyD HEPCGN_16115 0.40 +0.8 1,995,523 - slyD HEPCGN_16115 0.41 +1.0 1,995,530 + slyD HEPCGN_16115 0.42 -0.1 1,995,535 - slyD HEPCGN_16115 0.43 +0.8 1,995,557 + slyD HEPCGN_16115 0.46 -0.2 1,995,558 - slyD HEPCGN_16115 0.47 +0.4 1,995,558 - slyD HEPCGN_16115 0.47 +0.4 1,995,632 + slyD HEPCGN_16115 0.59 +0.7 1,995,632 + slyD HEPCGN_16115 0.59 -0.7 1,995,632 + slyD HEPCGN_16115 0.59 -0.3 1,995,646 - slyD HEPCGN_16115 0.61 +0.3 1,995,850 - -0.5 1,995,871 + -0.1 1,995,872 - +0.7 1,995,928 - -1.7 1,995,986 + slyX HEPCGN_16120 0.29 +0.2 1,995,987 - slyX HEPCGN_16120 0.29 -0.7 1,996,130 + +0.9 1,996,232 + -0.1 1,996,253 + -0.0 1,996,253 + -0.7 1,996,254 - +1.3 1,996,260 + +1.9 1,996,260 + +0.8 1,996,260 + +0.2 1,996,261 - -1.3 1,996,261 - -0.3 1,996,261 - -0.9 1,996,261 - -0.5 1,996,263 + -1.1 1,996,263 + -0.5 1,996,264 - +0.1 1,996,264 - -1.1 1,996,276 + -0.2 1,996,277 - +0.5 1,996,277 - -0.8 1,996,301 + +0.5 1,996,301 + -0.7 1,996,302 - +1.0 1,996,303 + -0.6 1,996,306 + +0.1 1,996,306 + -0.5 1,996,320 + -0.1 1,996,320 + -0.3 1,996,321 - +0.3 1,996,321 - -0.5 1,996,321 - -0.6 1,996,321 - -0.1 1,996,324 + +0.6 1,996,325 - +0.0 1,996,358 - +1.7 1,996,376 + +0.8 1,996,381 + -0.3 1,996,536 + fkpA HEPCGN_16125 0.21 +0.4 1,996,536 + fkpA HEPCGN_16125 0.21 +0.5 1,996,536 + fkpA HEPCGN_16125 0.21 +0.1 1,996,536 + fkpA HEPCGN_16125 0.21 +0.4 1,996,536 + fkpA HEPCGN_16125 0.21 +0.8 1,996,536 + fkpA HEPCGN_16125 0.21 +0.9 1,996,537 - fkpA HEPCGN_16125 0.22 -0.7 1,996,537 - fkpA HEPCGN_16125 0.22 +0.9 1,996,819 + fkpA HEPCGN_16125 0.56 +1.6 1,996,820 - fkpA HEPCGN_16125 0.56 -1.4 1,996,820 - fkpA HEPCGN_16125 0.56 +0.5 1,996,856 + fkpA HEPCGN_16125 0.61 +0.8 1,996,856 + fkpA HEPCGN_16125 0.61 -0.5
Or see this region's nucleotide sequence