Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgbU and ulaD overlap by 8 nucleotides ulaD and lyxK overlap by 4 nucleotides lyxK and yiaO are separated by 3 nucleotides
HEPCGN_14750: sgbU - Putative L-ribulose-5-phosphate 3-epimerase SgbU, at 1,709,361 to 1,710,221
sgbU
HEPCGN_14755: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 1,710,214 to 1,710,876
ulaD
HEPCGN_14760: lyxK - L-xylulokinase, at 1,710,873 to 1,712,369
lyxK
HEPCGN_14765: yiaO - 2,3-diketo-L-gulonate transporter substrate-binding protein YiaO, at 1,712,373 to 1,713,359
yiaO
Position (kb) 1710
1711
1712
1713 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1710.197 kb on - strand at 1710.197 kb on - strand at 1710.215 kb on + strand at 1710.216 kb on - strand at 1710.337 kb on - strand, within ulaD at 1710.411 kb on + strand, within ulaD at 1710.412 kb on - strand, within ulaD at 1710.412 kb on - strand, within ulaD at 1710.496 kb on - strand, within ulaD at 1710.634 kb on - strand, within ulaD at 1710.876 kb on + strand at 1710.877 kb on - strand at 1710.877 kb on - strand at 1710.877 kb on - strand at 1710.955 kb on + strand at 1711.005 kb on + strand at 1711.005 kb on + strand at 1711.005 kb on + strand at 1711.006 kb on - strand at 1711.197 kb on - strand, within lyxK at 1711.252 kb on + strand, within lyxK at 1711.253 kb on - strand, within lyxK at 1711.253 kb on - strand, within lyxK at 1711.499 kb on + strand, within lyxK at 1711.499 kb on + strand, within lyxK at 1711.513 kb on + strand, within lyxK at 1711.513 kb on + strand, within lyxK at 1711.513 kb on + strand, within lyxK at 1711.519 kb on + strand, within lyxK at 1711.519 kb on + strand, within lyxK at 1711.519 kb on + strand, within lyxK at 1711.520 kb on - strand, within lyxK at 1711.520 kb on - strand, within lyxK at 1711.520 kb on - strand, within lyxK at 1711.520 kb on - strand, within lyxK at 1711.520 kb on - strand, within lyxK at 1711.555 kb on - strand, within lyxK at 1711.592 kb on + strand, within lyxK at 1711.798 kb on + strand, within lyxK at 1711.886 kb on - strand, within lyxK at 1712.076 kb on - strand, within lyxK at 1712.076 kb on - strand, within lyxK at 1712.138 kb on + strand, within lyxK at 1712.139 kb on - strand, within lyxK at 1712.181 kb on - strand, within lyxK at 1712.181 kb on - strand, within lyxK at 1712.312 kb on - strand at 1712.347 kb on - strand at 1712.374 kb on + strand at 1712.426 kb on + strand at 1712.563 kb on + strand, within yiaO at 1712.563 kb on + strand, within yiaO at 1712.564 kb on - strand, within yiaO at 1712.564 kb on - strand, within yiaO at 1712.564 kb on - strand, within yiaO at 1712.773 kb on + strand, within yiaO at 1712.843 kb on + strand, within yiaO at 1713.123 kb on + strand, within yiaO at 1713.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 1,710,197 - -0.7 1,710,197 - -1.3 1,710,215 + +0.2 1,710,216 - +0.2 1,710,337 - ulaD HEPCGN_14755 0.19 -0.5 1,710,411 + ulaD HEPCGN_14755 0.30 +1.2 1,710,412 - ulaD HEPCGN_14755 0.30 +0.3 1,710,412 - ulaD HEPCGN_14755 0.30 +2.4 1,710,496 - ulaD HEPCGN_14755 0.43 +0.4 1,710,634 - ulaD HEPCGN_14755 0.63 -0.5 1,710,876 + +0.3 1,710,877 - -2.1 1,710,877 - -1.3 1,710,877 - -0.5 1,710,955 + -0.4 1,711,005 + -0.9 1,711,005 + +0.3 1,711,005 + +0.8 1,711,006 - -0.2 1,711,197 - lyxK HEPCGN_14760 0.22 -1.7 1,711,252 + lyxK HEPCGN_14760 0.25 -0.2 1,711,253 - lyxK HEPCGN_14760 0.25 -0.9 1,711,253 - lyxK HEPCGN_14760 0.25 -0.6 1,711,499 + lyxK HEPCGN_14760 0.42 -0.8 1,711,499 + lyxK HEPCGN_14760 0.42 -0.0 1,711,513 + lyxK HEPCGN_14760 0.43 +0.6 1,711,513 + lyxK HEPCGN_14760 0.43 -0.1 1,711,513 + lyxK HEPCGN_14760 0.43 +0.6 1,711,519 + lyxK HEPCGN_14760 0.43 -1.4 1,711,519 + lyxK HEPCGN_14760 0.43 +0.3 1,711,519 + lyxK HEPCGN_14760 0.43 +0.4 1,711,520 - lyxK HEPCGN_14760 0.43 -0.1 1,711,520 - lyxK HEPCGN_14760 0.43 +0.3 1,711,520 - lyxK HEPCGN_14760 0.43 +0.9 1,711,520 - lyxK HEPCGN_14760 0.43 -0.2 1,711,520 - lyxK HEPCGN_14760 0.43 -0.6 1,711,555 - lyxK HEPCGN_14760 0.46 -1.2 1,711,592 + lyxK HEPCGN_14760 0.48 -0.5 1,711,798 + lyxK HEPCGN_14760 0.62 -0.8 1,711,886 - lyxK HEPCGN_14760 0.68 -0.5 1,712,076 - lyxK HEPCGN_14760 0.80 -0.1 1,712,076 - lyxK HEPCGN_14760 0.80 -0.3 1,712,138 + lyxK HEPCGN_14760 0.85 +0.0 1,712,139 - lyxK HEPCGN_14760 0.85 +0.1 1,712,181 - lyxK HEPCGN_14760 0.87 -1.9 1,712,181 - lyxK HEPCGN_14760 0.87 -4.0 1,712,312 - +0.8 1,712,347 - -0.1 1,712,374 + -1.2 1,712,426 + +2.1 1,712,563 + yiaO HEPCGN_14765 0.19 +0.4 1,712,563 + yiaO HEPCGN_14765 0.19 +0.1 1,712,564 - yiaO HEPCGN_14765 0.19 -0.3 1,712,564 - yiaO HEPCGN_14765 0.19 +0.1 1,712,564 - yiaO HEPCGN_14765 0.19 -2.1 1,712,773 + yiaO HEPCGN_14765 0.41 -0.4 1,712,843 + yiaO HEPCGN_14765 0.48 -0.2 1,713,123 + yiaO HEPCGN_14765 0.76 -0.5 1,713,303 - -1.5
Or see this region's nucleotide sequence