Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdB and HEPCGN_10580 are separated by 10 nucleotides HEPCGN_10580 and frdD are separated by 10 nucleotides frdD and blaEC are separated by 62 nucleotides blaEC and blc are separated by 87 nucleotides blc and sugE overlap by 4 nucleotides
HEPCGN_10575: frdB - fumarate reductase iron-sulfur protein, at 858,989 to 859,723
frdB
HEPCGN_10580: HEPCGN_10580 - Fumarate reductase subunit C, at 859,734 to 860,129
_10580
HEPCGN_10585: frdD - fumarate reductase subunit FrdD, at 860,140 to 860,499
frdD
HEPCGN_10590: blaEC - BlaEC family class C beta-lactamase, at 860,562 to 861,695
blaEC
HEPCGN_10595: blc - lipocalin Blc, at 861,783 to 862,316
blc
HEPCGN_10600: sugE - quaternary ammonium compound efflux SMR transporter SugE, at 862,313 to 862,630
sugE
Position (kb) 860
861
862 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 859.603 kb on + strand, within frdB at 859.603 kb on + strand, within frdB at 859.677 kb on + strand at 859.748 kb on + strand at 859.755 kb on + strand at 859.755 kb on + strand at 859.755 kb on + strand at 859.755 kb on + strand at 859.756 kb on - strand at 859.756 kb on - strand at 859.759 kb on + strand at 859.759 kb on + strand at 859.759 kb on + strand at 859.759 kb on + strand at 859.759 kb on + strand at 859.809 kb on + strand, within HEPCGN_10580 at 860.022 kb on - strand, within HEPCGN_10580 at 860.101 kb on + strand at 860.206 kb on - strand, within frdD at 860.293 kb on + strand, within frdD at 860.336 kb on + strand, within frdD at 860.379 kb on - strand, within frdD at 860.385 kb on + strand, within frdD at 860.386 kb on - strand, within frdD at 860.420 kb on + strand, within frdD at 860.458 kb on - strand, within frdD at 860.490 kb on + strand at 860.490 kb on + strand at 860.497 kb on + strand at 860.498 kb on - strand at 860.528 kb on + strand at 860.561 kb on + strand at 860.562 kb on - strand at 860.587 kb on + strand at 860.588 kb on - strand at 860.590 kb on + strand at 860.590 kb on + strand at 860.591 kb on - strand at 860.639 kb on + strand at 860.658 kb on - strand at 860.669 kb on - strand at 860.704 kb on + strand, within blaEC at 860.727 kb on + strand, within blaEC at 860.732 kb on + strand, within blaEC at 860.742 kb on + strand, within blaEC at 860.840 kb on + strand, within blaEC at 860.840 kb on + strand, within blaEC at 860.864 kb on - strand, within blaEC at 860.884 kb on - strand, within blaEC at 860.926 kb on + strand, within blaEC at 860.926 kb on + strand, within blaEC at 860.927 kb on - strand, within blaEC at 860.930 kb on - strand, within blaEC at 860.930 kb on - strand, within blaEC at 860.930 kb on - strand, within blaEC at 860.930 kb on - strand, within blaEC at 861.012 kb on + strand, within blaEC at 861.057 kb on + strand, within blaEC at 861.167 kb on + strand, within blaEC at 861.168 kb on - strand, within blaEC at 861.177 kb on - strand, within blaEC at 861.177 kb on - strand, within blaEC at 861.182 kb on - strand, within blaEC at 861.205 kb on - strand, within blaEC at 861.235 kb on + strand, within blaEC at 861.236 kb on - strand, within blaEC at 861.236 kb on - strand, within blaEC at 861.256 kb on + strand, within blaEC at 861.257 kb on - strand, within blaEC at 861.270 kb on + strand, within blaEC at 861.270 kb on + strand, within blaEC at 861.406 kb on - strand, within blaEC at 861.532 kb on + strand, within blaEC at 861.551 kb on + strand, within blaEC at 861.551 kb on + strand, within blaEC at 861.551 kb on + strand, within blaEC at 861.552 kb on - strand, within blaEC at 861.552 kb on - strand, within blaEC at 861.582 kb on + strand at 861.582 kb on + strand at 861.582 kb on + strand at 861.583 kb on - strand at 861.583 kb on - strand at 861.583 kb on - strand at 861.583 kb on - strand at 861.583 kb on - strand at 861.583 kb on - strand at 861.640 kb on - strand at 861.689 kb on - strand at 861.751 kb on + strand at 861.752 kb on - strand at 861.757 kb on + strand at 861.782 kb on + strand at 861.782 kb on + strand at 861.848 kb on + strand, within blc at 861.879 kb on - strand, within blc at 861.972 kb on + strand, within blc at 861.973 kb on - strand, within blc at 862.007 kb on + strand, within blc at 862.010 kb on + strand, within blc at 862.011 kb on - strand, within blc at 862.018 kb on - strand, within blc at 862.018 kb on - strand, within blc at 862.018 kb on - strand, within blc at 862.062 kb on + strand, within blc at 862.129 kb on - strand, within blc at 862.131 kb on - strand, within blc at 862.131 kb on - strand, within blc at 862.136 kb on + strand, within blc at 862.191 kb on + strand, within blc at 862.192 kb on - strand, within blc at 862.202 kb on - strand, within blc at 862.283 kb on + strand at 862.284 kb on - strand at 862.284 kb on - strand at 862.289 kb on + strand at 862.297 kb on + strand at 862.339 kb on + strand at 862.340 kb on - strand at 862.529 kb on - strand, within sugE at 862.566 kb on + strand, within sugE at 862.610 kb on - strand at 862.618 kb on + strand at 862.618 kb on + strand at 862.618 kb on + strand at 862.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 859,603 + frdB HEPCGN_10575 0.84 +0.6 859,603 + frdB HEPCGN_10575 0.84 -1.1 859,677 + -0.4 859,748 + -0.1 859,755 + -0.4 859,755 + -1.3 859,755 + +0.3 859,755 + -0.3 859,756 - -1.3 859,756 - +0.1 859,759 + +1.2 859,759 + -0.1 859,759 + -0.7 859,759 + -0.1 859,759 + +1.2 859,809 + HEPCGN_10580 0.19 +1.4 860,022 - HEPCGN_10580 0.73 +0.4 860,101 + +0.4 860,206 - frdD HEPCGN_10585 0.18 +0.4 860,293 + frdD HEPCGN_10585 0.42 +0.2 860,336 + frdD HEPCGN_10585 0.54 -0.1 860,379 - frdD HEPCGN_10585 0.66 -0.3 860,385 + frdD HEPCGN_10585 0.68 -0.5 860,386 - frdD HEPCGN_10585 0.68 +2.7 860,420 + frdD HEPCGN_10585 0.78 -0.1 860,458 - frdD HEPCGN_10585 0.88 +1.4 860,490 + -1.0 860,490 + -1.5 860,497 + -1.5 860,498 - +0.3 860,528 + -0.0 860,561 + -0.7 860,562 - +1.4 860,587 + -0.5 860,588 - -1.2 860,590 + -0.3 860,590 + -0.4 860,591 - -0.1 860,639 + +0.0 860,658 - -1.2 860,669 - +0.5 860,704 + blaEC HEPCGN_10590 0.13 -0.5 860,727 + blaEC HEPCGN_10590 0.15 -0.1 860,732 + blaEC HEPCGN_10590 0.15 +1.5 860,742 + blaEC HEPCGN_10590 0.16 -2.5 860,840 + blaEC HEPCGN_10590 0.25 -0.8 860,840 + blaEC HEPCGN_10590 0.25 -1.6 860,864 - blaEC HEPCGN_10590 0.27 +1.8 860,884 - blaEC HEPCGN_10590 0.28 -0.7 860,926 + blaEC HEPCGN_10590 0.32 -1.0 860,926 + blaEC HEPCGN_10590 0.32 -0.3 860,927 - blaEC HEPCGN_10590 0.32 +0.2 860,930 - blaEC HEPCGN_10590 0.32 -0.3 860,930 - blaEC HEPCGN_10590 0.32 -0.3 860,930 - blaEC HEPCGN_10590 0.32 -0.3 860,930 - blaEC HEPCGN_10590 0.32 +0.6 861,012 + blaEC HEPCGN_10590 0.40 -0.0 861,057 + blaEC HEPCGN_10590 0.44 +0.2 861,167 + blaEC HEPCGN_10590 0.53 -0.1 861,168 - blaEC HEPCGN_10590 0.53 +0.7 861,177 - blaEC HEPCGN_10590 0.54 +0.9 861,177 - blaEC HEPCGN_10590 0.54 -0.1 861,182 - blaEC HEPCGN_10590 0.55 +0.6 861,205 - blaEC HEPCGN_10590 0.57 -0.1 861,235 + blaEC HEPCGN_10590 0.59 -0.4 861,236 - blaEC HEPCGN_10590 0.59 -0.8 861,236 - blaEC HEPCGN_10590 0.59 -0.1 861,256 + blaEC HEPCGN_10590 0.61 +0.1 861,257 - blaEC HEPCGN_10590 0.61 +2.0 861,270 + blaEC HEPCGN_10590 0.62 +0.1 861,270 + blaEC HEPCGN_10590 0.62 -1.1 861,406 - blaEC HEPCGN_10590 0.74 +0.3 861,532 + blaEC HEPCGN_10590 0.86 -1.6 861,551 + blaEC HEPCGN_10590 0.87 +0.1 861,551 + blaEC HEPCGN_10590 0.87 +0.9 861,551 + blaEC HEPCGN_10590 0.87 +0.2 861,552 - blaEC HEPCGN_10590 0.87 -0.1 861,552 - blaEC HEPCGN_10590 0.87 -0.7 861,582 + +0.4 861,582 + -0.8 861,582 + -0.7 861,583 - +2.8 861,583 - -0.1 861,583 - +2.3 861,583 - -0.3 861,583 - -0.8 861,583 - -3.0 861,640 - -0.4 861,689 - -0.2 861,751 + +0.5 861,752 - -1.7 861,757 + +0.2 861,782 + -0.4 861,782 + +0.3 861,848 + blc HEPCGN_10595 0.12 +0.6 861,879 - blc HEPCGN_10595 0.18 +0.8 861,972 + blc HEPCGN_10595 0.35 -1.9 861,973 - blc HEPCGN_10595 0.36 -0.0 862,007 + blc HEPCGN_10595 0.42 +0.9 862,010 + blc HEPCGN_10595 0.43 -0.4 862,011 - blc HEPCGN_10595 0.43 -0.2 862,018 - blc HEPCGN_10595 0.44 -0.5 862,018 - blc HEPCGN_10595 0.44 -0.3 862,018 - blc HEPCGN_10595 0.44 -0.2 862,062 + blc HEPCGN_10595 0.52 -2.0 862,129 - blc HEPCGN_10595 0.65 -0.8 862,131 - blc HEPCGN_10595 0.65 +0.2 862,131 - blc HEPCGN_10595 0.65 +2.1 862,136 + blc HEPCGN_10595 0.66 +1.9 862,191 + blc HEPCGN_10595 0.76 -0.4 862,192 - blc HEPCGN_10595 0.77 -0.8 862,202 - blc HEPCGN_10595 0.78 +0.0 862,283 + -0.1 862,284 - +0.3 862,284 - +0.5 862,289 + -1.1 862,297 + -0.6 862,339 + +0.6 862,340 - +0.3 862,529 - sugE HEPCGN_10600 0.68 -0.6 862,566 + sugE HEPCGN_10600 0.80 -0.5 862,610 - -0.0 862,618 + +1.0 862,618 + -0.7 862,618 + -0.8 862,631 - +0.2
Or see this region's nucleotide sequence