Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yeeZ are separated by 426 nucleotides
HEPCGN_03220: hisD - Histidinol dehydrogenase, at 555,681 to 556,985
hisD
HEPCGN_03225: hisG - ATP phosphoribosyltransferase, at 556,991 to 557,890
hisG
HEPCGN_03230: hisL - his operon leader peptide, at 558,036 to 558,086
hisL
HEPCGN_03235: yeeZ - Protein YeeZ, at 558,513 to 559,337
yeeZ
Position (kb) 556
557
558 Strain fitness (log2 ratio) -2
-1
0
1
2 at 556.292 kb on + strand, within hisD at 556.323 kb on + strand, within hisD at 556.598 kb on - strand, within hisD at 556.598 kb on - strand, within hisD at 556.598 kb on - strand, within hisD at 556.615 kb on - strand, within hisD at 556.641 kb on + strand, within hisD at 556.659 kb on + strand, within hisD at 556.659 kb on + strand, within hisD at 556.704 kb on - strand, within hisD at 556.734 kb on + strand, within hisD at 556.735 kb on - strand, within hisD at 556.825 kb on + strand, within hisD at 556.955 kb on + strand at 556.955 kb on + strand at 556.956 kb on - strand at 556.976 kb on + strand at 557.102 kb on + strand, within hisG at 557.134 kb on + strand, within hisG at 557.208 kb on + strand, within hisG at 557.329 kb on + strand, within hisG at 557.329 kb on + strand, within hisG at 557.330 kb on - strand, within hisG at 557.353 kb on + strand, within hisG at 557.442 kb on - strand, within hisG at 557.499 kb on + strand, within hisG at 557.512 kb on + strand, within hisG at 557.800 kb on + strand, within hisG at 557.865 kb on - strand at 557.896 kb on + strand at 557.897 kb on - strand at 558.164 kb on + strand at 558.164 kb on + strand at 558.229 kb on + strand at 558.230 kb on - strand at 558.230 kb on - strand at 558.255 kb on - strand at 558.294 kb on - strand at 558.368 kb on - strand at 558.368 kb on - strand at 558.415 kb on - strand at 558.417 kb on - strand at 558.471 kb on + strand at 558.482 kb on + strand at 558.484 kb on + strand at 558.485 kb on - strand at 558.485 kb on - strand at 558.485 kb on - strand at 558.539 kb on - strand at 558.651 kb on - strand, within yeeZ at 558.654 kb on + strand, within yeeZ at 558.680 kb on - strand, within yeeZ at 558.779 kb on - strand, within yeeZ
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 556,292 + hisD HEPCGN_03220 0.47 -0.3 556,323 + hisD HEPCGN_03220 0.49 -0.7 556,598 - hisD HEPCGN_03220 0.70 +0.4 556,598 - hisD HEPCGN_03220 0.70 +0.3 556,598 - hisD HEPCGN_03220 0.70 -1.8 556,615 - hisD HEPCGN_03220 0.72 -0.2 556,641 + hisD HEPCGN_03220 0.74 +0.1 556,659 + hisD HEPCGN_03220 0.75 +1.0 556,659 + hisD HEPCGN_03220 0.75 -0.0 556,704 - hisD HEPCGN_03220 0.78 -0.2 556,734 + hisD HEPCGN_03220 0.81 -1.4 556,735 - hisD HEPCGN_03220 0.81 +0.3 556,825 + hisD HEPCGN_03220 0.88 +0.3 556,955 + -0.3 556,955 + +0.2 556,956 - -0.2 556,976 + +0.3 557,102 + hisG HEPCGN_03225 0.12 +2.7 557,134 + hisG HEPCGN_03225 0.16 +0.5 557,208 + hisG HEPCGN_03225 0.24 +0.8 557,329 + hisG HEPCGN_03225 0.38 +0.7 557,329 + hisG HEPCGN_03225 0.38 -0.7 557,330 - hisG HEPCGN_03225 0.38 +0.3 557,353 + hisG HEPCGN_03225 0.40 +0.4 557,442 - hisG HEPCGN_03225 0.50 -2.7 557,499 + hisG HEPCGN_03225 0.56 -0.0 557,512 + hisG HEPCGN_03225 0.58 -0.3 557,800 + hisG HEPCGN_03225 0.90 +1.0 557,865 - -0.9 557,896 + +0.1 557,897 - +0.3 558,164 + -2.4 558,164 + +0.9 558,229 + +0.9 558,230 - +0.8 558,230 - +1.6 558,255 - -1.2 558,294 - -0.5 558,368 - +0.2 558,368 - +0.2 558,415 - +0.2 558,417 - +1.6 558,471 + -0.3 558,482 + -0.3 558,484 + -0.8 558,485 - +0.7 558,485 - -1.0 558,485 - -0.0 558,539 - -0.8 558,651 - yeeZ HEPCGN_03235 0.17 -1.8 558,654 + yeeZ HEPCGN_03235 0.17 -0.6 558,680 - yeeZ HEPCGN_03235 0.20 -0.3 558,779 - yeeZ HEPCGN_03235 0.32 -1.7
Or see this region's nucleotide sequence