Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjJ and rcdA are separated by 83 nucleotides rcdA and ybjL are separated by 174 nucleotides ybjL and ybjM are separated by 269 nucleotides ybjM and grxA are separated by 29 nucleotides
HEPCGN_02020: ybjJ - Inner membrane protein YbjJ, at 330,718 to 331,926
ybjJ
HEPCGN_02025: rcdA - DNA-binding transcriptional regulator RcdA, at 332,010 to 332,546
rcdA
HEPCGN_02035: ybjL - aspartate:alanine antiporter, at 332,721 to 334,406
ybjL
HEPCGN_02040: ybjM - Inner membrane protein YbjM, at 334,676 to 335,053
ybjM
HEPCGN_02045: grxA - glutaredoxin 1, at 335,083 to 335,340
grxA
Position (kb) 332
333
334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 331.807 kb on - strand at 331.906 kb on - strand at 331.926 kb on + strand at 331.942 kb on - strand at 331.962 kb on + strand at 332.086 kb on + strand, within rcdA at 332.087 kb on - strand, within rcdA at 332.148 kb on + strand, within rcdA at 332.287 kb on - strand, within rcdA at 332.328 kb on + strand, within rcdA at 332.329 kb on - strand, within rcdA at 332.365 kb on + strand, within rcdA at 332.457 kb on - strand, within rcdA at 332.547 kb on + strand at 332.791 kb on + strand at 332.791 kb on + strand at 332.791 kb on + strand at 332.791 kb on + strand at 332.803 kb on + strand at 332.834 kb on + strand at 332.916 kb on + strand, within ybjL at 332.916 kb on + strand, within ybjL at 332.917 kb on - strand, within ybjL at 332.917 kb on - strand, within ybjL at 332.920 kb on + strand, within ybjL at 332.920 kb on + strand, within ybjL at 332.920 kb on + strand, within ybjL at 332.921 kb on - strand, within ybjL at 332.921 kb on - strand, within ybjL at 333.014 kb on + strand, within ybjL at 333.015 kb on - strand, within ybjL at 333.144 kb on - strand, within ybjL at 333.360 kb on - strand, within ybjL at 333.403 kb on - strand, within ybjL at 333.588 kb on + strand, within ybjL at 333.589 kb on - strand, within ybjL at 333.615 kb on + strand, within ybjL at 333.616 kb on - strand, within ybjL at 333.692 kb on - strand, within ybjL at 333.732 kb on + strand, within ybjL at 333.841 kb on - strand, within ybjL at 333.986 kb on + strand, within ybjL at 334.073 kb on + strand, within ybjL at 334.078 kb on - strand, within ybjL at 334.129 kb on - strand, within ybjL at 334.129 kb on - strand, within ybjL at 334.208 kb on + strand, within ybjL at 334.208 kb on + strand, within ybjL at 334.260 kb on + strand at 334.370 kb on - strand at 334.523 kb on - strand at 334.531 kb on - strand at 334.538 kb on + strand at 334.538 kb on + strand at 334.568 kb on + strand at 334.586 kb on - strand at 334.590 kb on + strand at 334.613 kb on + strand at 334.613 kb on + strand at 334.618 kb on + strand at 334.685 kb on - strand at 334.906 kb on + strand, within ybjM at 334.965 kb on + strand, within ybjM at 334.966 kb on - strand, within ybjM at 334.966 kb on - strand, within ybjM at 334.966 kb on - strand, within ybjM at 335.008 kb on - strand, within ybjM at 335.011 kb on + strand, within ybjM at 335.126 kb on + strand, within grxA at 335.198 kb on - strand, within grxA at 335.229 kb on + strand, within grxA at 335.230 kb on - strand, within grxA at 335.233 kb on + strand, within grxA at 335.233 kb on + strand, within grxA at 335.233 kb on + strand, within grxA at 335.234 kb on - strand, within grxA at 335.234 kb on - strand, within grxA at 335.238 kb on - strand, within grxA at 335.238 kb on - strand, within grxA at 335.304 kb on - strand, within grxA at 335.304 kb on - strand, within grxA at 335.356 kb on - strand at 335.361 kb on + strand at 335.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 331,807 - -0.7 331,906 - -1.0 331,926 + -0.5 331,942 - -1.4 331,962 + +1.9 332,086 + rcdA HEPCGN_02025 0.14 -0.3 332,087 - rcdA HEPCGN_02025 0.14 +3.4 332,148 + rcdA HEPCGN_02025 0.26 -0.9 332,287 - rcdA HEPCGN_02025 0.52 +0.8 332,328 + rcdA HEPCGN_02025 0.59 -0.5 332,329 - rcdA HEPCGN_02025 0.59 +0.8 332,365 + rcdA HEPCGN_02025 0.66 +0.1 332,457 - rcdA HEPCGN_02025 0.83 +1.2 332,547 + -0.5 332,791 + +1.4 332,791 + +2.1 332,791 + -0.9 332,791 + -0.4 332,803 + -0.5 332,834 + +1.5 332,916 + ybjL HEPCGN_02035 0.12 +0.2 332,916 + ybjL HEPCGN_02035 0.12 +0.5 332,917 - ybjL HEPCGN_02035 0.12 +1.0 332,917 - ybjL HEPCGN_02035 0.12 +0.3 332,920 + ybjL HEPCGN_02035 0.12 +0.2 332,920 + ybjL HEPCGN_02035 0.12 -0.2 332,920 + ybjL HEPCGN_02035 0.12 +0.7 332,921 - ybjL HEPCGN_02035 0.12 +1.8 332,921 - ybjL HEPCGN_02035 0.12 -0.1 333,014 + ybjL HEPCGN_02035 0.17 -0.7 333,015 - ybjL HEPCGN_02035 0.17 +0.5 333,144 - ybjL HEPCGN_02035 0.25 -1.3 333,360 - ybjL HEPCGN_02035 0.38 -0.9 333,403 - ybjL HEPCGN_02035 0.40 +1.6 333,588 + ybjL HEPCGN_02035 0.51 -0.1 333,589 - ybjL HEPCGN_02035 0.51 -0.8 333,615 + ybjL HEPCGN_02035 0.53 -0.8 333,616 - ybjL HEPCGN_02035 0.53 -1.5 333,692 - ybjL HEPCGN_02035 0.58 -3.3 333,732 + ybjL HEPCGN_02035 0.60 +1.5 333,841 - ybjL HEPCGN_02035 0.66 -0.9 333,986 + ybjL HEPCGN_02035 0.75 -0.9 334,073 + ybjL HEPCGN_02035 0.80 +0.2 334,078 - ybjL HEPCGN_02035 0.80 -0.5 334,129 - ybjL HEPCGN_02035 0.84 +0.6 334,129 - ybjL HEPCGN_02035 0.84 +1.3 334,208 + ybjL HEPCGN_02035 0.88 -1.6 334,208 + ybjL HEPCGN_02035 0.88 +1.9 334,260 + -0.9 334,370 - +0.5 334,523 - +0.4 334,531 - -0.2 334,538 + -2.1 334,538 + -0.3 334,568 + +0.1 334,586 - +1.1 334,590 + -0.6 334,613 + -0.7 334,613 + +0.5 334,618 + +1.2 334,685 - -0.5 334,906 + ybjM HEPCGN_02040 0.61 +0.7 334,965 + ybjM HEPCGN_02040 0.76 -0.1 334,966 - ybjM HEPCGN_02040 0.77 +1.1 334,966 - ybjM HEPCGN_02040 0.77 +1.1 334,966 - ybjM HEPCGN_02040 0.77 -0.4 335,008 - ybjM HEPCGN_02040 0.88 +0.3 335,011 + ybjM HEPCGN_02040 0.89 +0.2 335,126 + grxA HEPCGN_02045 0.17 -0.4 335,198 - grxA HEPCGN_02045 0.45 +1.1 335,229 + grxA HEPCGN_02045 0.57 +0.5 335,230 - grxA HEPCGN_02045 0.57 +0.1 335,233 + grxA HEPCGN_02045 0.58 +0.3 335,233 + grxA HEPCGN_02045 0.58 -0.3 335,233 + grxA HEPCGN_02045 0.58 -0.6 335,234 - grxA HEPCGN_02045 0.59 +0.3 335,234 - grxA HEPCGN_02045 0.59 -0.6 335,238 - grxA HEPCGN_02045 0.60 -0.3 335,238 - grxA HEPCGN_02045 0.60 +0.5 335,304 - grxA HEPCGN_02045 0.86 -1.9 335,304 - grxA HEPCGN_02045 0.86 -0.4 335,356 - +0.2 335,361 + -0.3 335,362 - -0.4
Or see this region's nucleotide sequence