Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00525 and ybcY are separated by 193 nucleotides ybcY and HEPCGN_00535 are separated by 68 nucleotides HEPCGN_00535 and menH are separated by 281 nucleotides
HEPCGN_00525: HEPCGN_00525 - hypothetical protein, at 28,104 to 28,397
_00525
HEPCGN_00530: ybcY - Putative uncharacterized protein YbcY, at 28,591 to 29,247
ybcY
HEPCGN_00535: HEPCGN_00535 - Phage tail fiber assembly protein, at 29,316 to 29,516
_00535
HEPCGN_00540: menH - alpha/beta hydrolase, at 29,798 to 31,282
menH
Position (kb)
29
30 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 28.485 kb on + strand at 28.485 kb on + strand at 28.485 kb on + strand at 28.485 kb on + strand at 28.503 kb on + strand at 28.548 kb on - strand at 28.619 kb on - strand at 28.621 kb on + strand at 28.670 kb on - strand, within ybcY at 28.671 kb on + strand, within ybcY at 28.678 kb on + strand, within ybcY at 28.794 kb on - strand, within ybcY at 28.814 kb on - strand, within ybcY at 28.835 kb on - strand, within ybcY at 28.838 kb on - strand, within ybcY at 28.838 kb on - strand, within ybcY at 28.838 kb on - strand, within ybcY at 28.916 kb on - strand, within ybcY at 28.956 kb on + strand, within ybcY at 28.986 kb on + strand, within ybcY at 28.986 kb on + strand, within ybcY at 29.048 kb on - strand, within ybcY at 29.097 kb on + strand, within ybcY at 29.304 kb on - strand at 29.427 kb on + strand, within HEPCGN_00535 at 29.427 kb on + strand, within HEPCGN_00535 at 29.427 kb on + strand, within HEPCGN_00535 at 29.428 kb on - strand, within HEPCGN_00535 at 29.429 kb on + strand, within HEPCGN_00535 at 29.430 kb on - strand, within HEPCGN_00535 at 29.528 kb on - strand at 29.528 kb on - strand at 29.535 kb on + strand at 29.536 kb on - strand at 29.607 kb on + strand at 29.701 kb on - strand at 29.917 kb on + strand at 30.015 kb on - strand, within menH at 30.073 kb on + strand, within menH at 30.074 kb on - strand, within menH at 30.074 kb on - strand, within menH at 30.077 kb on + strand, within menH at 30.078 kb on - strand, within menH at 30.078 kb on - strand, within menH at 30.210 kb on - strand, within menH at 30.286 kb on + strand, within menH at 30.286 kb on + strand, within menH at 30.395 kb on - strand, within menH at 30.413 kb on + strand, within menH at 30.443 kb on + strand, within menH at 30.444 kb on - strand, within menH at 30.462 kb on - strand, within menH
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 28,485 + -0.7 28,485 + +0.6 28,485 + +0.6 28,485 + +0.1 28,503 + +0.5 28,548 - +0.3 28,619 - -0.7 28,621 + +1.0 28,670 - ybcY HEPCGN_00530 0.12 -0.1 28,671 + ybcY HEPCGN_00530 0.12 +0.8 28,678 + ybcY HEPCGN_00530 0.13 +0.0 28,794 - ybcY HEPCGN_00530 0.31 +0.9 28,814 - ybcY HEPCGN_00530 0.34 -0.7 28,835 - ybcY HEPCGN_00530 0.37 -0.8 28,838 - ybcY HEPCGN_00530 0.38 -1.0 28,838 - ybcY HEPCGN_00530 0.38 +0.8 28,838 - ybcY HEPCGN_00530 0.38 +2.1 28,916 - ybcY HEPCGN_00530 0.49 +0.4 28,956 + ybcY HEPCGN_00530 0.56 -2.9 28,986 + ybcY HEPCGN_00530 0.60 +0.3 28,986 + ybcY HEPCGN_00530 0.60 -0.7 29,048 - ybcY HEPCGN_00530 0.70 -0.1 29,097 + ybcY HEPCGN_00530 0.77 -0.0 29,304 - -0.0 29,427 + HEPCGN_00535 0.55 +0.0 29,427 + HEPCGN_00535 0.55 -1.1 29,427 + HEPCGN_00535 0.55 -0.4 29,428 - HEPCGN_00535 0.56 +0.5 29,429 + HEPCGN_00535 0.56 +2.2 29,430 - HEPCGN_00535 0.57 -0.7 29,528 - -0.5 29,528 - -0.9 29,535 + +0.2 29,536 - +1.4 29,607 + +0.2 29,701 - +0.3 29,917 + -1.0 30,015 - menH HEPCGN_00540 0.15 +0.2 30,073 + menH HEPCGN_00540 0.19 +0.5 30,074 - menH HEPCGN_00540 0.19 +0.2 30,074 - menH HEPCGN_00540 0.19 -0.4 30,077 + menH HEPCGN_00540 0.19 +1.1 30,078 - menH HEPCGN_00540 0.19 -1.7 30,078 - menH HEPCGN_00540 0.19 -0.3 30,210 - menH HEPCGN_00540 0.28 +1.3 30,286 + menH HEPCGN_00540 0.33 -0.4 30,286 + menH HEPCGN_00540 0.33 -0.3 30,395 - menH HEPCGN_00540 0.40 +0.5 30,413 + menH HEPCGN_00540 0.41 +2.1 30,443 + menH HEPCGN_00540 0.43 +1.8 30,444 - menH HEPCGN_00540 0.44 +0.8 30,462 - menH HEPCGN_00540 0.45 +0.1
Or see this region's nucleotide sequence