Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00045 and iss are separated by 168 nucleotides iss and rzpD are separated by 31 nucleotides rzpD and rrrD overlap by 4 nucleotides rrrD and essD overlap by 1 nucleotides essD and HEPCGN_00070 are separated by 188 nucleotides
HEPCGN_00045: HEPCGN_00045 - Arginyl-tRNA synthetase, at 133 to 288
_00045
HEPCGN_00050: iss - increased serum survival lipoprotein Iss, at 457 to 750
iss
HEPCGN_00055: rzpD - prophage endopeptidase RzpD, at 782 to 1,243
rzpD
HEPCGN_00060: rrrD - lysozyme RrrD, at 1,240 to 1,737
rrrD
HEPCGN_00065: essD - phage lysis protein EssD, at 1,737 to 1,952
essD
HEPCGN_00070: HEPCGN_00070 - AraC family transcriptional regulator, at 2,141 to 2,872
_00070
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2 at 0.042 kb on + strand at 0.092 kb on - strand at 0.156 kb on + strand, within HEPCGN_00045 at 0.165 kb on + strand, within HEPCGN_00045 at 0.350 kb on - strand at 0.350 kb on - strand at 0.366 kb on + strand at 0.697 kb on - strand, within iss at 0.699 kb on + strand, within iss at 0.712 kb on + strand, within iss at 0.714 kb on + strand, within iss at 0.749 kb on - strand at 0.784 kb on - strand at 0.804 kb on + strand at 0.805 kb on - strand at 1.146 kb on + strand, within rzpD at 1.150 kb on + strand, within rzpD at 1.175 kb on - strand, within rzpD at 1.319 kb on + strand, within rrrD at 1.333 kb on + strand, within rrrD at 1.472 kb on + strand, within rrrD at 1.541 kb on + strand, within rrrD at 1.605 kb on + strand, within rrrD at 1.605 kb on + strand, within rrrD at 1.614 kb on - strand, within rrrD at 1.619 kb on - strand, within rrrD at 1.619 kb on - strand, within rrrD at 1.623 kb on - strand, within rrrD at 1.678 kb on + strand, within rrrD at 1.738 kb on + strand at 1.772 kb on + strand, within essD at 1.816 kb on - strand, within essD at 1.816 kb on - strand, within essD at 1.836 kb on + strand, within essD at 1.837 kb on - strand, within essD at 1.837 kb on - strand, within essD at 1.915 kb on + strand, within essD at 1.938 kb on - strand at 2.009 kb on + strand at 2.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 42 + -1.1 92 - +0.8 156 + HEPCGN_00045 0.15 -0.4 165 + HEPCGN_00045 0.21 +0.2 350 - -0.1 350 - -1.3 366 + +0.9 697 - iss HEPCGN_00050 0.82 -0.2 699 + iss HEPCGN_00050 0.82 +0.6 712 + iss HEPCGN_00050 0.87 -0.3 714 + iss HEPCGN_00050 0.87 +0.1 749 - +0.2 784 - -0.1 804 + -0.1 805 - +1.2 1,146 + rzpD HEPCGN_00055 0.79 -0.9 1,150 + rzpD HEPCGN_00055 0.80 -1.0 1,175 - rzpD HEPCGN_00055 0.85 -1.7 1,319 + rrrD HEPCGN_00060 0.16 -1.2 1,333 + rrrD HEPCGN_00060 0.19 -0.5 1,472 + rrrD HEPCGN_00060 0.47 +0.2 1,541 + rrrD HEPCGN_00060 0.60 -0.6 1,605 + rrrD HEPCGN_00060 0.73 -0.5 1,605 + rrrD HEPCGN_00060 0.73 -0.1 1,614 - rrrD HEPCGN_00060 0.75 -0.7 1,619 - rrrD HEPCGN_00060 0.76 -1.7 1,619 - rrrD HEPCGN_00060 0.76 -0.5 1,623 - rrrD HEPCGN_00060 0.77 -1.9 1,678 + rrrD HEPCGN_00060 0.88 +0.2 1,738 + +0.7 1,772 + essD HEPCGN_00065 0.16 -0.3 1,816 - essD HEPCGN_00065 0.37 -1.2 1,816 - essD HEPCGN_00065 0.37 +2.2 1,836 + essD HEPCGN_00065 0.46 +0.6 1,837 - essD HEPCGN_00065 0.46 -1.2 1,837 - essD HEPCGN_00065 0.46 -1.0 1,915 + essD HEPCGN_00065 0.82 +1.0 1,938 - -0.1 2,009 + -0.9 2,012 - -0.6
Or see this region's nucleotide sequence