Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serB and ahpA are separated by 105 nucleotides ahpA and lplA are separated by 27 nucleotides lplA and HEPCGN_09215 are separated by 31 nucleotides HEPCGN_09215 and deoD are separated by 160 nucleotides
HEPCGN_09200: serB - phosphoserine phosphatase, at 588,328 to 589,296
serB
HEPCGN_09205: ahpA - YtjB family periplasmic protein, at 589,402 to 590,046
ahpA
HEPCGN_09210: lplA - lipoate--protein ligase LplA, at 590,074 to 591,090
lplA
HEPCGN_09215: HEPCGN_09215 - transcriptional regulator, at 591,122 to 591,385
_09215
HEPCGN_09220: deoD - purine-nucleoside phosphorylase, at 591,546 to 592,265
deoD
Position (kb) 590
591
592 Strain fitness (log2 ratio) -2
-1
0
1
2 at 589.076 kb on + strand, within serB at 589.110 kb on + strand, within serB at 589.133 kb on + strand, within serB at 589.169 kb on + strand, within serB at 589.169 kb on + strand, within serB at 589.200 kb on - strand at 589.333 kb on - strand at 589.346 kb on - strand at 589.353 kb on - strand at 589.353 kb on - strand at 589.353 kb on - strand at 589.353 kb on - strand at 589.374 kb on + strand at 589.374 kb on + strand at 589.375 kb on - strand at 589.375 kb on - strand at 589.375 kb on - strand at 589.375 kb on - strand at 589.524 kb on + strand, within ahpA at 589.769 kb on - strand, within ahpA at 589.774 kb on - strand, within ahpA at 589.774 kb on - strand, within ahpA at 589.849 kb on - strand, within ahpA at 589.849 kb on - strand, within ahpA at 589.887 kb on + strand, within ahpA at 589.888 kb on - strand, within ahpA at 589.895 kb on + strand, within ahpA at 589.895 kb on + strand, within ahpA at 590.052 kb on + strand at 590.052 kb on + strand at 590.052 kb on + strand at 590.053 kb on - strand at 590.055 kb on + strand at 590.056 kb on - strand at 590.084 kb on + strand at 590.085 kb on - strand at 590.085 kb on - strand at 590.143 kb on - strand at 590.241 kb on + strand, within lplA at 590.241 kb on + strand, within lplA at 590.265 kb on + strand, within lplA at 590.265 kb on + strand, within lplA at 590.334 kb on + strand, within lplA at 590.608 kb on - strand, within lplA at 590.707 kb on + strand, within lplA at 590.793 kb on + strand, within lplA at 590.899 kb on - strand, within lplA at 591.121 kb on + strand at 591.162 kb on + strand, within HEPCGN_09215 at 591.163 kb on - strand, within HEPCGN_09215 at 591.163 kb on - strand, within HEPCGN_09215 at 591.163 kb on - strand, within HEPCGN_09215 at 591.179 kb on - strand, within HEPCGN_09215 at 591.180 kb on + strand, within HEPCGN_09215 at 591.180 kb on + strand, within HEPCGN_09215 at 591.180 kb on + strand, within HEPCGN_09215 at 591.181 kb on - strand, within HEPCGN_09215 at 591.181 kb on - strand, within HEPCGN_09215 at 591.181 kb on - strand, within HEPCGN_09215 at 591.211 kb on + strand, within HEPCGN_09215 at 591.211 kb on + strand, within HEPCGN_09215 at 591.212 kb on - strand, within HEPCGN_09215 at 591.217 kb on - strand, within HEPCGN_09215 at 591.232 kb on - strand, within HEPCGN_09215 at 591.247 kb on + strand, within HEPCGN_09215 at 591.248 kb on - strand, within HEPCGN_09215 at 591.256 kb on + strand, within HEPCGN_09215 at 591.327 kb on - strand, within HEPCGN_09215 at 591.351 kb on + strand, within HEPCGN_09215 at 591.351 kb on + strand, within HEPCGN_09215 at 591.379 kb on + strand at 591.380 kb on - strand at 591.403 kb on + strand at 591.477 kb on - strand at 591.547 kb on + strand at 591.711 kb on - strand, within deoD at 591.785 kb on - strand, within deoD at 591.857 kb on + strand, within deoD at 591.902 kb on + strand, within deoD at 591.903 kb on - strand, within deoD
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 589,076 + serB HEPCGN_09200 0.77 +1.3 589,110 + serB HEPCGN_09200 0.81 +0.4 589,133 + serB HEPCGN_09200 0.83 -0.4 589,169 + serB HEPCGN_09200 0.87 +2.6 589,169 + serB HEPCGN_09200 0.87 -0.0 589,200 - -0.0 589,333 - -0.2 589,346 - -0.4 589,353 - -0.6 589,353 - -0.2 589,353 - -0.8 589,353 - +1.6 589,374 + +0.9 589,374 + +0.2 589,375 - -0.4 589,375 - +0.1 589,375 - -2.3 589,375 - +0.8 589,524 + ahpA HEPCGN_09205 0.19 -1.4 589,769 - ahpA HEPCGN_09205 0.57 +1.1 589,774 - ahpA HEPCGN_09205 0.58 -1.0 589,774 - ahpA HEPCGN_09205 0.58 -0.5 589,849 - ahpA HEPCGN_09205 0.69 -1.4 589,849 - ahpA HEPCGN_09205 0.69 -0.2 589,887 + ahpA HEPCGN_09205 0.75 -2.6 589,888 - ahpA HEPCGN_09205 0.75 -0.9 589,895 + ahpA HEPCGN_09205 0.76 -1.0 589,895 + ahpA HEPCGN_09205 0.76 -0.2 590,052 + -0.0 590,052 + +0.3 590,052 + +1.2 590,053 - -2.2 590,055 + +0.4 590,056 - +1.2 590,084 + +0.7 590,085 - +1.0 590,085 - +0.1 590,143 - -1.3 590,241 + lplA HEPCGN_09210 0.16 +1.0 590,241 + lplA HEPCGN_09210 0.16 +0.6 590,265 + lplA HEPCGN_09210 0.19 +0.1 590,265 + lplA HEPCGN_09210 0.19 +0.9 590,334 + lplA HEPCGN_09210 0.26 -1.6 590,608 - lplA HEPCGN_09210 0.53 +1.0 590,707 + lplA HEPCGN_09210 0.62 +0.5 590,793 + lplA HEPCGN_09210 0.71 +0.1 590,899 - lplA HEPCGN_09210 0.81 +0.9 591,121 + -0.2 591,162 + HEPCGN_09215 0.15 -0.6 591,163 - HEPCGN_09215 0.16 -0.7 591,163 - HEPCGN_09215 0.16 +0.1 591,163 - HEPCGN_09215 0.16 +0.3 591,179 - HEPCGN_09215 0.22 +0.4 591,180 + HEPCGN_09215 0.22 +0.6 591,180 + HEPCGN_09215 0.22 -1.7 591,180 + HEPCGN_09215 0.22 +0.3 591,181 - HEPCGN_09215 0.22 +0.0 591,181 - HEPCGN_09215 0.22 +1.6 591,181 - HEPCGN_09215 0.22 +1.3 591,211 + HEPCGN_09215 0.34 -1.2 591,211 + HEPCGN_09215 0.34 -0.1 591,212 - HEPCGN_09215 0.34 +0.1 591,217 - HEPCGN_09215 0.36 +0.1 591,232 - HEPCGN_09215 0.42 -0.7 591,247 + HEPCGN_09215 0.47 +0.5 591,248 - HEPCGN_09215 0.48 +0.3 591,256 + HEPCGN_09215 0.51 +0.7 591,327 - HEPCGN_09215 0.78 -0.5 591,351 + HEPCGN_09215 0.87 -0.0 591,351 + HEPCGN_09215 0.87 -0.1 591,379 + +0.2 591,380 - -0.2 591,403 + +0.9 591,477 - +0.7 591,547 + -0.9 591,711 - deoD HEPCGN_09220 0.23 -1.6 591,785 - deoD HEPCGN_09220 0.33 -2.0 591,857 + deoD HEPCGN_09220 0.43 -1.4 591,902 + deoD HEPCGN_09220 0.49 -0.6 591,903 - deoD HEPCGN_09220 0.50 -0.1
Or see this region's nucleotide sequence