Strain Fitness in Escherichia coli ECOR38 around HEPCGN_08695

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntleuO and leuL are separated by 658 nucleotidesleuL and HEPCGN_08695 are separated by 92 nucleotidesHEPCGN_08695 and leuB overlap by 1 nucleotides HEPCGN_08685: leuO - transcriptional regulator LeuO, at 477,083 to 478,027 leuO HEPCGN_08690: leuL - leu operon leader peptide, at 478,686 to 478,772 leuL HEPCGN_08695: HEPCGN_08695 - 2-isopropylmalate synthase, at 478,865 to 480,436 _08695 HEPCGN_08700: leuB - 3-isopropylmalate dehydrogenase, at 480,436 to 481,527 leuB Position (kb) 478 479 480 481Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 478.027 kb on + strandat 478.027 kb on + strandat 478.050 kb on - strandat 478.114 kb on - strandat 478.295 kb on - strandat 478.323 kb on - strandat 478.470 kb on - strandat 478.566 kb on + strandat 478.715 kb on - strand, within leuLat 478.717 kb on + strand, within leuLat 478.720 kb on + strand, within leuLat 478.721 kb on - strand, within leuLat 479.074 kb on - strand, within HEPCGN_08695at 479.082 kb on + strand, within HEPCGN_08695at 479.156 kb on - strand, within HEPCGN_08695at 479.156 kb on - strand, within HEPCGN_08695at 479.156 kb on - strand, within HEPCGN_08695at 479.162 kb on - strand, within HEPCGN_08695at 479.230 kb on + strand, within HEPCGN_08695at 479.231 kb on - strand, within HEPCGN_08695at 479.255 kb on - strand, within HEPCGN_08695at 479.426 kb on + strand, within HEPCGN_08695at 479.426 kb on + strand, within HEPCGN_08695at 479.467 kb on - strand, within HEPCGN_08695at 479.467 kb on - strand, within HEPCGN_08695at 479.470 kb on + strand, within HEPCGN_08695at 479.471 kb on - strand, within HEPCGN_08695at 479.471 kb on - strand, within HEPCGN_08695at 479.517 kb on + strand, within HEPCGN_08695at 479.576 kb on - strand, within HEPCGN_08695at 479.639 kb on - strand, within HEPCGN_08695at 479.680 kb on - strand, within HEPCGN_08695at 479.684 kb on - strand, within HEPCGN_08695at 479.696 kb on - strand, within HEPCGN_08695at 479.818 kb on + strand, within HEPCGN_08695at 479.818 kb on + strand, within HEPCGN_08695at 479.901 kb on - strand, within HEPCGN_08695at 479.903 kb on - strand, within HEPCGN_08695at 479.903 kb on - strand, within HEPCGN_08695at 479.903 kb on - strand, within HEPCGN_08695at 479.918 kb on - strand, within HEPCGN_08695at 479.981 kb on + strand, within HEPCGN_08695at 479.981 kb on + strand, within HEPCGN_08695at 479.981 kb on + strand, within HEPCGN_08695at 479.982 kb on - strand, within HEPCGN_08695at 479.982 kb on - strand, within HEPCGN_08695at 479.982 kb on - strand, within HEPCGN_08695at 480.161 kb on + strand, within HEPCGN_08695at 480.162 kb on - strand, within HEPCGN_08695at 480.235 kb on + strand, within HEPCGN_08695at 480.272 kb on + strand, within HEPCGN_08695at 480.273 kb on - strand, within HEPCGN_08695at 480.273 kb on - strand, within HEPCGN_08695at 480.313 kb on + strandat 480.348 kb on + strandat 480.348 kb on + strandat 480.349 kb on - strandat 480.358 kb on + strandat 480.448 kb on + strandat 480.448 kb on + strandat 480.448 kb on + strandat 480.448 kb on + strandat 480.563 kb on - strand, within leuBat 480.589 kb on - strand, within leuBat 480.663 kb on - strand, within leuBat 480.733 kb on - strand, within leuBat 480.935 kb on - strand, within leuBat 480.935 kb on - strand, within leuBat 480.935 kb on - strand, within leuBat 480.935 kb on - strand, within leuBat 481.011 kb on + strand, within leuBat 481.104 kb on + strand, within leuBat 481.104 kb on + strand, within leuBat 481.108 kb on + strand, within leuBat 481.109 kb on - strand, within leuB

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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478,027 + +0.2
478,027 + -0.4
478,050 - +0.0
478,114 - +0.5
478,295 - +2.1
478,323 - +0.2
478,470 - +0.4
478,566 + +0.0
478,715 - leuL HEPCGN_08690 0.33 +2.3
478,717 + leuL HEPCGN_08690 0.36 -2.5
478,720 + leuL HEPCGN_08690 0.39 -0.3
478,721 - leuL HEPCGN_08690 0.40 +0.6
479,074 - HEPCGN_08695 0.13 +2.6
479,082 + HEPCGN_08695 0.14 -0.4
479,156 - HEPCGN_08695 0.19 -0.6
479,156 - HEPCGN_08695 0.19 +1.2
479,156 - HEPCGN_08695 0.19 +1.5
479,162 - HEPCGN_08695 0.19 -0.1
479,230 + HEPCGN_08695 0.23 +0.0
479,231 - HEPCGN_08695 0.23 -0.4
479,255 - HEPCGN_08695 0.25 -0.5
479,426 + HEPCGN_08695 0.36 -0.3
479,426 + HEPCGN_08695 0.36 +0.5
479,467 - HEPCGN_08695 0.38 +0.8
479,467 - HEPCGN_08695 0.38 +0.8
479,470 + HEPCGN_08695 0.38 +0.9
479,471 - HEPCGN_08695 0.39 -0.0
479,471 - HEPCGN_08695 0.39 -1.0
479,517 + HEPCGN_08695 0.41 +0.5
479,576 - HEPCGN_08695 0.45 -1.3
479,639 - HEPCGN_08695 0.49 -0.9
479,680 - HEPCGN_08695 0.52 -0.7
479,684 - HEPCGN_08695 0.52 +0.3
479,696 - HEPCGN_08695 0.53 +0.5
479,818 + HEPCGN_08695 0.61 +1.6
479,818 + HEPCGN_08695 0.61 +0.8
479,901 - HEPCGN_08695 0.66 +0.3
479,903 - HEPCGN_08695 0.66 +0.4
479,903 - HEPCGN_08695 0.66 +0.7
479,903 - HEPCGN_08695 0.66 +0.5
479,918 - HEPCGN_08695 0.67 -0.2
479,981 + HEPCGN_08695 0.71 -0.6
479,981 + HEPCGN_08695 0.71 -0.6
479,981 + HEPCGN_08695 0.71 +1.1
479,982 - HEPCGN_08695 0.71 +0.5
479,982 - HEPCGN_08695 0.71 -0.5
479,982 - HEPCGN_08695 0.71 -0.4
480,161 + HEPCGN_08695 0.82 -1.4
480,162 - HEPCGN_08695 0.83 +0.1
480,235 + HEPCGN_08695 0.87 +0.5
480,272 + HEPCGN_08695 0.90 +1.5
480,273 - HEPCGN_08695 0.90 +1.5
480,273 - HEPCGN_08695 0.90 -0.4
480,313 + -0.2
480,348 + -0.6
480,348 + +1.5
480,349 - -0.1
480,358 + +0.3
480,448 + -1.1
480,448 + -0.9
480,448 + -0.0
480,448 + +0.3
480,563 - leuB HEPCGN_08700 0.12 +0.2
480,589 - leuB HEPCGN_08700 0.14 -0.5
480,663 - leuB HEPCGN_08700 0.21 +0.5
480,733 - leuB HEPCGN_08700 0.27 +0.4
480,935 - leuB HEPCGN_08700 0.46 +0.2
480,935 - leuB HEPCGN_08700 0.46 +0.3
480,935 - leuB HEPCGN_08700 0.46 -0.7
480,935 - leuB HEPCGN_08700 0.46 +3.8
481,011 + leuB HEPCGN_08700 0.53 +0.6
481,104 + leuB HEPCGN_08700 0.61 -0.0
481,104 + leuB HEPCGN_08700 0.61 +0.7
481,108 + leuB HEPCGN_08700 0.62 -0.8
481,109 - leuB HEPCGN_08700 0.62 +0.6

Or see this region's nucleotide sequence