Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06025

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfumB and fumC are separated by 142 nucleotidesfumC and tus overlap by 4 nucleotides HEPCGN_06020: fumB - class I fumarate hydratase, at 1,078,174 to 1,079,820 fumB HEPCGN_06025: fumC - class II fumarate hydratase, at 1,079,963 to 1,081,366 fumC HEPCGN_06030: tus - DNA replication terminus site-binding protein, at 1,081,363 to 1,082,292 tus Position (kb) 1079 1080 1081 1082Strain fitness (log2 ratio) -2 -1 0 1 2at 1079.046 kb on + strand, within fumBat 1079.050 kb on + strand, within fumBat 1079.140 kb on - strand, within fumBat 1079.193 kb on + strand, within fumBat 1079.206 kb on + strand, within fumBat 1079.206 kb on + strand, within fumBat 1079.206 kb on + strand, within fumBat 1079.207 kb on - strand, within fumBat 1079.207 kb on - strand, within fumBat 1079.263 kb on - strand, within fumBat 1079.465 kb on - strand, within fumBat 1079.513 kb on - strand, within fumBat 1079.513 kb on - strand, within fumBat 1079.544 kb on + strand, within fumBat 1079.545 kb on - strand, within fumBat 1079.545 kb on - strand, within fumBat 1079.623 kb on - strand, within fumBat 1079.626 kb on - strand, within fumBat 1079.645 kb on + strand, within fumBat 1079.662 kb on - strandat 1079.664 kb on + strandat 1079.665 kb on - strandat 1079.849 kb on + strandat 1079.968 kb on + strandat 1079.968 kb on + strandat 1079.969 kb on - strandat 1079.973 kb on + strandat 1080.020 kb on - strandat 1080.148 kb on - strand, within fumCat 1080.268 kb on + strand, within fumCat 1080.268 kb on + strand, within fumCat 1080.269 kb on - strand, within fumCat 1080.340 kb on + strand, within fumCat 1080.353 kb on - strand, within fumCat 1080.604 kb on + strand, within fumCat 1080.604 kb on + strand, within fumCat 1080.612 kb on - strand, within fumCat 1080.627 kb on + strand, within fumCat 1080.669 kb on + strand, within fumCat 1080.673 kb on + strand, within fumCat 1080.673 kb on + strand, within fumCat 1080.674 kb on - strand, within fumCat 1080.686 kb on + strand, within fumCat 1080.687 kb on - strand, within fumCat 1081.266 kb on + strandat 1081.267 kb on - strandat 1081.348 kb on + strandat 1081.376 kb on + strandat 1081.376 kb on + strandat 1081.426 kb on - strandat 1081.426 kb on - strandat 1081.428 kb on - strandat 1081.428 kb on - strandat 1081.764 kb on + strand, within tusat 1081.765 kb on - strand, within tusat 1081.765 kb on - strand, within tusat 1082.063 kb on - strand, within tusat 1082.128 kb on - strand, within tusat 1082.142 kb on + strand, within tusat 1082.295 kb on + strandat 1082.296 kb on - strandat 1082.298 kb on + strandat 1082.363 kb on + strandat 1082.364 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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1,079,046 + fumB HEPCGN_06020 0.53 -0.8
1,079,050 + fumB HEPCGN_06020 0.53 +0.1
1,079,140 - fumB HEPCGN_06020 0.59 -1.6
1,079,193 + fumB HEPCGN_06020 0.62 +0.2
1,079,206 + fumB HEPCGN_06020 0.63 -0.4
1,079,206 + fumB HEPCGN_06020 0.63 -1.5
1,079,206 + fumB HEPCGN_06020 0.63 +0.3
1,079,207 - fumB HEPCGN_06020 0.63 -0.3
1,079,207 - fumB HEPCGN_06020 0.63 -1.8
1,079,263 - fumB HEPCGN_06020 0.66 -0.3
1,079,465 - fumB HEPCGN_06020 0.78 +1.1
1,079,513 - fumB HEPCGN_06020 0.81 +0.3
1,079,513 - fumB HEPCGN_06020 0.81 -0.7
1,079,544 + fumB HEPCGN_06020 0.83 -0.4
1,079,545 - fumB HEPCGN_06020 0.83 +0.1
1,079,545 - fumB HEPCGN_06020 0.83 +1.6
1,079,623 - fumB HEPCGN_06020 0.88 -0.3
1,079,626 - fumB HEPCGN_06020 0.88 +0.1
1,079,645 + fumB HEPCGN_06020 0.89 -0.0
1,079,662 - +0.1
1,079,664 + +0.2
1,079,665 - +1.1
1,079,849 + +0.2
1,079,968 + +2.1
1,079,968 + +1.1
1,079,969 - -0.7
1,079,973 + -0.1
1,080,020 - +0.6
1,080,148 - fumC HEPCGN_06025 0.13 -1.1
1,080,268 + fumC HEPCGN_06025 0.22 -0.9
1,080,268 + fumC HEPCGN_06025 0.22 +0.0
1,080,269 - fumC HEPCGN_06025 0.22 -1.5
1,080,340 + fumC HEPCGN_06025 0.27 -0.4
1,080,353 - fumC HEPCGN_06025 0.28 -0.3
1,080,604 + fumC HEPCGN_06025 0.46 +0.1
1,080,604 + fumC HEPCGN_06025 0.46 +0.3
1,080,612 - fumC HEPCGN_06025 0.46 -0.4
1,080,627 + fumC HEPCGN_06025 0.47 +0.1
1,080,669 + fumC HEPCGN_06025 0.50 -0.4
1,080,673 + fumC HEPCGN_06025 0.51 -0.5
1,080,673 + fumC HEPCGN_06025 0.51 +0.5
1,080,674 - fumC HEPCGN_06025 0.51 -0.4
1,080,686 + fumC HEPCGN_06025 0.51 -0.5
1,080,687 - fumC HEPCGN_06025 0.52 +0.3
1,081,266 + -0.9
1,081,267 - -0.7
1,081,348 + +1.7
1,081,376 + +0.2
1,081,376 + +1.6
1,081,426 - -1.9
1,081,426 - +0.9
1,081,428 - -0.4
1,081,428 - -0.1
1,081,764 + tus HEPCGN_06030 0.43 -0.1
1,081,765 - tus HEPCGN_06030 0.43 +0.7
1,081,765 - tus HEPCGN_06030 0.43 +0.3
1,082,063 - tus HEPCGN_06030 0.75 +0.1
1,082,128 - tus HEPCGN_06030 0.82 -0.6
1,082,142 + tus HEPCGN_06030 0.84 -0.4
1,082,295 + -0.6
1,082,296 - -0.1
1,082,298 + +0.1
1,082,363 + -0.8
1,082,364 - -0.4

Or see this region's nucleotide sequence