Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumB and fumC are separated by 142 nucleotides fumC and tus overlap by 4 nucleotides
HEPCGN_06020: fumB - class I fumarate hydratase, at 1,078,174 to 1,079,820
fumB
HEPCGN_06025: fumC - class II fumarate hydratase, at 1,079,963 to 1,081,366
fumC
HEPCGN_06030: tus - DNA replication terminus site-binding protein, at 1,081,363 to 1,082,292
tus
Position (kb)
1079
1080
1081
1082 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1079.046 kb on + strand, within fumB at 1079.050 kb on + strand, within fumB at 1079.140 kb on - strand, within fumB at 1079.193 kb on + strand, within fumB at 1079.206 kb on + strand, within fumB at 1079.206 kb on + strand, within fumB at 1079.206 kb on + strand, within fumB at 1079.207 kb on - strand, within fumB at 1079.207 kb on - strand, within fumB at 1079.263 kb on - strand, within fumB at 1079.465 kb on - strand, within fumB at 1079.513 kb on - strand, within fumB at 1079.513 kb on - strand, within fumB at 1079.544 kb on + strand, within fumB at 1079.545 kb on - strand, within fumB at 1079.545 kb on - strand, within fumB at 1079.623 kb on - strand, within fumB at 1079.626 kb on - strand, within fumB at 1079.645 kb on + strand, within fumB at 1079.662 kb on - strand at 1079.664 kb on + strand at 1079.665 kb on - strand at 1079.849 kb on + strand at 1079.968 kb on + strand at 1079.968 kb on + strand at 1079.969 kb on - strand at 1079.973 kb on + strand at 1080.020 kb on - strand at 1080.148 kb on - strand, within fumC at 1080.268 kb on + strand, within fumC at 1080.268 kb on + strand, within fumC at 1080.269 kb on - strand, within fumC at 1080.340 kb on + strand, within fumC at 1080.353 kb on - strand, within fumC at 1080.604 kb on + strand, within fumC at 1080.604 kb on + strand, within fumC at 1080.612 kb on - strand, within fumC at 1080.627 kb on + strand, within fumC at 1080.669 kb on + strand, within fumC at 1080.673 kb on + strand, within fumC at 1080.673 kb on + strand, within fumC at 1080.674 kb on - strand, within fumC at 1080.686 kb on + strand, within fumC at 1080.687 kb on - strand, within fumC at 1081.266 kb on + strand at 1081.267 kb on - strand at 1081.348 kb on + strand at 1081.376 kb on + strand at 1081.376 kb on + strand at 1081.426 kb on - strand at 1081.426 kb on - strand at 1081.428 kb on - strand at 1081.428 kb on - strand at 1081.764 kb on + strand, within tus at 1081.765 kb on - strand, within tus at 1081.765 kb on - strand, within tus at 1082.063 kb on - strand, within tus at 1082.128 kb on - strand, within tus at 1082.142 kb on + strand, within tus at 1082.295 kb on + strand at 1082.296 kb on - strand at 1082.298 kb on + strand at 1082.363 kb on + strand at 1082.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 1,079,046 + fumB HEPCGN_06020 0.53 -0.8 1,079,050 + fumB HEPCGN_06020 0.53 +0.1 1,079,140 - fumB HEPCGN_06020 0.59 -1.6 1,079,193 + fumB HEPCGN_06020 0.62 +0.2 1,079,206 + fumB HEPCGN_06020 0.63 -0.4 1,079,206 + fumB HEPCGN_06020 0.63 -1.5 1,079,206 + fumB HEPCGN_06020 0.63 +0.3 1,079,207 - fumB HEPCGN_06020 0.63 -0.3 1,079,207 - fumB HEPCGN_06020 0.63 -1.8 1,079,263 - fumB HEPCGN_06020 0.66 -0.3 1,079,465 - fumB HEPCGN_06020 0.78 +1.1 1,079,513 - fumB HEPCGN_06020 0.81 +0.3 1,079,513 - fumB HEPCGN_06020 0.81 -0.7 1,079,544 + fumB HEPCGN_06020 0.83 -0.4 1,079,545 - fumB HEPCGN_06020 0.83 +0.1 1,079,545 - fumB HEPCGN_06020 0.83 +1.6 1,079,623 - fumB HEPCGN_06020 0.88 -0.3 1,079,626 - fumB HEPCGN_06020 0.88 +0.1 1,079,645 + fumB HEPCGN_06020 0.89 -0.0 1,079,662 - +0.1 1,079,664 + +0.2 1,079,665 - +1.1 1,079,849 + +0.2 1,079,968 + +2.1 1,079,968 + +1.1 1,079,969 - -0.7 1,079,973 + -0.1 1,080,020 - +0.6 1,080,148 - fumC HEPCGN_06025 0.13 -1.1 1,080,268 + fumC HEPCGN_06025 0.22 -0.9 1,080,268 + fumC HEPCGN_06025 0.22 +0.0 1,080,269 - fumC HEPCGN_06025 0.22 -1.5 1,080,340 + fumC HEPCGN_06025 0.27 -0.4 1,080,353 - fumC HEPCGN_06025 0.28 -0.3 1,080,604 + fumC HEPCGN_06025 0.46 +0.1 1,080,604 + fumC HEPCGN_06025 0.46 +0.3 1,080,612 - fumC HEPCGN_06025 0.46 -0.4 1,080,627 + fumC HEPCGN_06025 0.47 +0.1 1,080,669 + fumC HEPCGN_06025 0.50 -0.4 1,080,673 + fumC HEPCGN_06025 0.51 -0.5 1,080,673 + fumC HEPCGN_06025 0.51 +0.5 1,080,674 - fumC HEPCGN_06025 0.51 -0.4 1,080,686 + fumC HEPCGN_06025 0.51 -0.5 1,080,687 - fumC HEPCGN_06025 0.52 +0.3 1,081,266 + -0.9 1,081,267 - -0.7 1,081,348 + +1.7 1,081,376 + +0.2 1,081,376 + +1.6 1,081,426 - -1.9 1,081,426 - +0.9 1,081,428 - -0.4 1,081,428 - -0.1 1,081,764 + tus HEPCGN_06030 0.43 -0.1 1,081,765 - tus HEPCGN_06030 0.43 +0.7 1,081,765 - tus HEPCGN_06030 0.43 +0.3 1,082,063 - tus HEPCGN_06030 0.75 +0.1 1,082,128 - tus HEPCGN_06030 0.82 -0.6 1,082,142 + tus HEPCGN_06030 0.84 -0.4 1,082,295 + -0.6 1,082,296 - -0.1 1,082,298 + +0.1 1,082,363 + -0.8 1,082,364 - -0.4
Or see this region's nucleotide sequence