Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyk and hexR are separated by 127 nucleotides hexR and HEPCGN_04735 are separated by 22 nucleotides HEPCGN_04735 and zwf are separated by 164 nucleotides
HEPCGN_04725: pyk - pyruvate kinase, at 823,718 to 825,160
pyk
HEPCGN_04730: hexR - DNA-binding transcriptional regulator HexR, at 825,288 to 826,157
hexR
HEPCGN_04735: HEPCGN_04735 - hypothetical protein, at 826,180 to 826,329
_04735
HEPCGN_04740: zwf - glucose-6-phosphate dehydrogenase, at 826,494 to 827,969
zwf
Position (kb) 825
826
827 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 824.424 kb on - strand, within pyk at 824.545 kb on - strand, within pyk at 824.587 kb on - strand, within pyk at 824.614 kb on + strand, within pyk at 824.677 kb on + strand, within pyk at 824.820 kb on + strand, within pyk at 824.918 kb on - strand, within pyk at 824.927 kb on + strand, within pyk at 825.094 kb on + strand at 825.162 kb on + strand at 825.162 kb on + strand at 825.162 kb on + strand at 825.162 kb on + strand at 825.162 kb on + strand at 825.163 kb on - strand at 825.163 kb on - strand at 825.163 kb on - strand at 825.163 kb on - strand at 825.166 kb on - strand at 825.219 kb on + strand at 825.260 kb on + strand at 825.309 kb on - strand at 825.313 kb on - strand at 825.417 kb on + strand, within hexR at 825.418 kb on - strand, within hexR at 825.418 kb on - strand, within hexR at 825.455 kb on + strand, within hexR at 825.456 kb on - strand, within hexR at 825.456 kb on - strand, within hexR at 825.456 kb on - strand, within hexR at 825.457 kb on + strand, within hexR at 825.457 kb on + strand, within hexR at 825.457 kb on + strand, within hexR at 825.458 kb on - strand, within hexR at 825.468 kb on - strand, within hexR at 825.544 kb on - strand, within hexR at 825.549 kb on - strand, within hexR at 825.605 kb on + strand, within hexR at 825.648 kb on - strand, within hexR at 825.648 kb on - strand, within hexR at 825.671 kb on + strand, within hexR at 825.699 kb on - strand, within hexR at 825.713 kb on + strand, within hexR at 825.750 kb on + strand, within hexR at 825.920 kb on - strand, within hexR at 826.229 kb on + strand, within HEPCGN_04735 at 826.229 kb on + strand, within HEPCGN_04735 at 826.230 kb on - strand, within HEPCGN_04735 at 826.288 kb on - strand, within HEPCGN_04735 at 826.413 kb on - strand at 826.421 kb on + strand at 826.422 kb on - strand at 826.449 kb on - strand at 826.451 kb on - strand at 826.476 kb on + strand at 826.477 kb on - strand at 826.482 kb on - strand at 826.655 kb on + strand, within zwf at 826.665 kb on + strand, within zwf at 826.666 kb on - strand, within zwf at 826.839 kb on + strand, within zwf at 826.921 kb on + strand, within zwf at 827.010 kb on + strand, within zwf
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 824,424 - pyk HEPCGN_04725 0.49 +4.4 824,545 - pyk HEPCGN_04725 0.57 -0.2 824,587 - pyk HEPCGN_04725 0.60 +0.8 824,614 + pyk HEPCGN_04725 0.62 +2.1 824,677 + pyk HEPCGN_04725 0.66 +0.5 824,820 + pyk HEPCGN_04725 0.76 +0.5 824,918 - pyk HEPCGN_04725 0.83 -0.1 824,927 + pyk HEPCGN_04725 0.84 +0.9 825,094 + -1.0 825,162 + +0.3 825,162 + +2.1 825,162 + +2.3 825,162 + +1.2 825,162 + +0.1 825,163 - -1.0 825,163 - +2.0 825,163 - +0.5 825,163 - +1.5 825,166 - +0.6 825,219 + +2.6 825,260 + -0.6 825,309 - +0.6 825,313 - +0.9 825,417 + hexR HEPCGN_04730 0.15 +0.6 825,418 - hexR HEPCGN_04730 0.15 -0.2 825,418 - hexR HEPCGN_04730 0.15 +0.2 825,455 + hexR HEPCGN_04730 0.19 +0.2 825,456 - hexR HEPCGN_04730 0.19 -1.3 825,456 - hexR HEPCGN_04730 0.19 +0.6 825,456 - hexR HEPCGN_04730 0.19 -2.3 825,457 + hexR HEPCGN_04730 0.19 -0.2 825,457 + hexR HEPCGN_04730 0.19 +0.9 825,457 + hexR HEPCGN_04730 0.19 +0.3 825,458 - hexR HEPCGN_04730 0.20 +1.7 825,468 - hexR HEPCGN_04730 0.21 +0.6 825,544 - hexR HEPCGN_04730 0.29 -0.7 825,549 - hexR HEPCGN_04730 0.30 +3.4 825,605 + hexR HEPCGN_04730 0.36 +1.2 825,648 - hexR HEPCGN_04730 0.41 +1.0 825,648 - hexR HEPCGN_04730 0.41 +0.5 825,671 + hexR HEPCGN_04730 0.44 +0.5 825,699 - hexR HEPCGN_04730 0.47 -0.9 825,713 + hexR HEPCGN_04730 0.49 +0.0 825,750 + hexR HEPCGN_04730 0.53 +0.0 825,920 - hexR HEPCGN_04730 0.73 +0.4 826,229 + HEPCGN_04735 0.33 -0.3 826,229 + HEPCGN_04735 0.33 -1.1 826,230 - HEPCGN_04735 0.33 -0.4 826,288 - HEPCGN_04735 0.72 -2.3 826,413 - +5.5 826,421 + -0.2 826,422 - +1.5 826,449 - +1.5 826,451 - +1.9 826,476 + +0.0 826,477 - +2.5 826,482 - +1.8 826,655 + zwf HEPCGN_04740 0.11 +2.7 826,665 + zwf HEPCGN_04740 0.12 +2.7 826,666 - zwf HEPCGN_04740 0.12 +2.1 826,839 + zwf HEPCGN_04740 0.23 -0.4 826,921 + zwf HEPCGN_04740 0.29 +2.6 827,010 + zwf HEPCGN_04740 0.35 +2.5
Or see this region's nucleotide sequence