Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_02420 and Ile2_trna are separated by 40 nucleotides Ile2_trna and Arg_trna are separated by 9 nucleotides Arg_trna and tolA are separated by 278 nucleotides tolA and HEPCGN_02450 are separated by 586 nucleotides
HEPCGN_02420: HEPCGN_02420 - Site-specific DNA-methyltransferase, at 395,207 to 395,485
_02420
HEPCGN_02425: Ile2_trna - tRNA-Ile2(cat), at 395,526 to 395,601
_trna
HEPCGN_02430: Arg_trna - tRNA-Arg(tcg), at 395,611 to 395,687
_trna
HEPCGN_02435: tolA - cell envelope integrity protein TolA, at 395,966 to 396,394
tolA
HEPCGN_02450: HEPCGN_02450 - 9-O-acetyl-N-acetylneuraminic acid deacetylase, at 396,981 to 398,942
_02450
Position (kb) 395
396
397 Strain fitness (log2 ratio) -2
-1
0
1
2 at 395.060 kb on - strand at 395.123 kb on - strand at 395.407 kb on + strand, within HEPCGN_02420 at 395.407 kb on + strand, within HEPCGN_02420 at 395.467 kb on - strand at 395.487 kb on - strand at 395.602 kb on + strand at 395.602 kb on + strand at 395.602 kb on + strand at 395.689 kb on + strand at 395.753 kb on + strand at 395.755 kb on + strand at 395.756 kb on - strand at 395.756 kb on - strand at 395.805 kb on - strand at 395.805 kb on - strand at 395.843 kb on + strand at 395.843 kb on + strand at 395.889 kb on - strand at 395.892 kb on + strand at 395.893 kb on - strand at 396.050 kb on + strand, within tolA at 396.082 kb on - strand, within tolA at 396.146 kb on + strand, within tolA at 396.183 kb on + strand, within tolA at 396.230 kb on + strand, within tolA at 396.352 kb on - strand at 396.352 kb on - strand at 396.385 kb on + strand at 396.418 kb on + strand at 396.654 kb on - strand at 396.813 kb on + strand at 396.813 kb on + strand at 396.958 kb on + strand at 396.959 kb on - strand at 397.101 kb on + strand at 397.209 kb on + strand, within HEPCGN_02450 at 397.210 kb on - strand, within HEPCGN_02450 at 397.213 kb on - strand, within HEPCGN_02450 at 397.285 kb on - strand, within HEPCGN_02450 at 397.285 kb on - strand, within HEPCGN_02450 at 397.285 kb on - strand, within HEPCGN_02450 at 397.308 kb on - strand, within HEPCGN_02450 at 397.359 kb on + strand, within HEPCGN_02450 at 397.359 kb on + strand, within HEPCGN_02450
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 395,060 - +2.3 395,123 - +1.8 395,407 + HEPCGN_02420 0.72 +1.5 395,407 + HEPCGN_02420 0.72 +1.0 395,467 - +0.6 395,487 - -0.1 395,602 + +0.6 395,602 + +0.9 395,602 + -0.1 395,689 + +0.0 395,753 + +0.6 395,755 + +0.1 395,756 - +2.0 395,756 - -0.7 395,805 - -1.1 395,805 - -0.6 395,843 + -0.1 395,843 + -0.4 395,889 - +0.4 395,892 + +1.0 395,893 - +1.2 396,050 + tolA HEPCGN_02435 0.20 -2.7 396,082 - tolA HEPCGN_02435 0.27 +0.4 396,146 + tolA HEPCGN_02435 0.42 -0.3 396,183 + tolA HEPCGN_02435 0.51 +0.2 396,230 + tolA HEPCGN_02435 0.62 -0.4 396,352 - +0.2 396,352 - +0.6 396,385 + -0.6 396,418 + +1.9 396,654 - -0.5 396,813 + -0.4 396,813 + +0.3 396,958 + +0.7 396,959 - +0.1 397,101 + -2.0 397,209 + HEPCGN_02450 0.12 -0.6 397,210 - HEPCGN_02450 0.12 +2.4 397,213 - HEPCGN_02450 0.12 +0.3 397,285 - HEPCGN_02450 0.15 +0.9 397,285 - HEPCGN_02450 0.15 -1.5 397,285 - HEPCGN_02450 0.15 +0.0 397,308 - HEPCGN_02450 0.17 -0.2 397,359 + HEPCGN_02450 0.19 -0.4 397,359 + HEPCGN_02450 0.19 -0.6
Or see this region's nucleotide sequence