Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_02050 and nfsA overlap by 17 nucleotides nfsA and rimK are separated by 60 nucleotides rimK and ybjN are separated by 87 nucleotides ybjN and potF are separated by 350 nucleotides
HEPCGN_02050: HEPCGN_02050 - DUF1418 domain-containing protein, at 335,500 to 335,787
_02050
HEPCGN_02055: nfsA - nitroreductase NfsA, at 335,771 to 336,493
nfsA
HEPCGN_02060: rimK - 30S ribosomal protein S6--L-glutamate ligase, at 336,554 to 337,456
rimK
HEPCGN_02065: ybjN - Uncharacterized protein YbjN, at 337,544 to 338,020
ybjN
HEPCGN_02070: potF - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 338,371 to 339,483
potF
Position (kb) 336
337
338 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 335.688 kb on + strand, within HEPCGN_02050 at 335.743 kb on + strand, within HEPCGN_02050 at 335.769 kb on + strand at 335.769 kb on + strand at 335.791 kb on + strand at 335.791 kb on + strand at 335.791 kb on + strand at 335.792 kb on - strand at 335.915 kb on - strand, within nfsA at 335.924 kb on - strand, within nfsA at 335.980 kb on - strand, within nfsA at 336.151 kb on - strand, within nfsA at 336.152 kb on + strand, within nfsA at 336.152 kb on + strand, within nfsA at 336.153 kb on - strand, within nfsA at 336.153 kb on - strand, within nfsA at 336.155 kb on - strand, within nfsA at 336.175 kb on + strand, within nfsA at 336.176 kb on - strand, within nfsA at 336.176 kb on - strand, within nfsA at 336.344 kb on + strand, within nfsA at 336.368 kb on + strand, within nfsA at 336.369 kb on - strand, within nfsA at 336.369 kb on - strand, within nfsA at 336.369 kb on - strand, within nfsA at 336.452 kb on - strand at 336.515 kb on + strand at 336.516 kb on - strand at 336.590 kb on + strand at 336.591 kb on - strand at 336.591 kb on - strand at 336.598 kb on + strand at 336.621 kb on + strand at 336.622 kb on - strand at 336.666 kb on - strand, within rimK at 336.676 kb on - strand, within rimK at 336.694 kb on + strand, within rimK at 336.694 kb on + strand, within rimK at 336.694 kb on + strand, within rimK at 336.717 kb on + strand, within rimK at 336.746 kb on - strand, within rimK at 336.761 kb on - strand, within rimK at 336.939 kb on + strand, within rimK at 336.989 kb on - strand, within rimK at 337.088 kb on + strand, within rimK at 337.088 kb on + strand, within rimK at 337.088 kb on + strand, within rimK at 337.088 kb on + strand, within rimK at 337.088 kb on + strand, within rimK at 337.089 kb on - strand, within rimK at 337.090 kb on + strand, within rimK at 337.298 kb on - strand, within rimK at 337.310 kb on - strand, within rimK at 337.363 kb on - strand, within rimK at 337.424 kb on - strand at 337.434 kb on - strand at 337.469 kb on + strand at 337.502 kb on - strand at 337.624 kb on + strand, within ybjN at 337.652 kb on - strand, within ybjN at 337.768 kb on - strand, within ybjN at 337.826 kb on - strand, within ybjN at 337.881 kb on - strand, within ybjN at 337.881 kb on - strand, within ybjN at 337.946 kb on - strand, within ybjN at 338.134 kb on + strand at 338.191 kb on + strand at 338.192 kb on - strand at 338.234 kb on - strand at 338.235 kb on + strand at 338.235 kb on + strand at 338.236 kb on - strand at 338.381 kb on + strand at 338.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 335,688 + HEPCGN_02050 0.65 +0.2 335,743 + HEPCGN_02050 0.84 -1.3 335,769 + +0.1 335,769 + +0.2 335,791 + +1.2 335,791 + +0.2 335,791 + -0.1 335,792 - -1.1 335,915 - nfsA HEPCGN_02055 0.20 -0.7 335,924 - nfsA HEPCGN_02055 0.21 +1.8 335,980 - nfsA HEPCGN_02055 0.29 -1.8 336,151 - nfsA HEPCGN_02055 0.53 +0.4 336,152 + nfsA HEPCGN_02055 0.53 -0.5 336,152 + nfsA HEPCGN_02055 0.53 +1.7 336,153 - nfsA HEPCGN_02055 0.53 -0.7 336,153 - nfsA HEPCGN_02055 0.53 -0.7 336,155 - nfsA HEPCGN_02055 0.53 -0.4 336,175 + nfsA HEPCGN_02055 0.56 +0.6 336,176 - nfsA HEPCGN_02055 0.56 -1.1 336,176 - nfsA HEPCGN_02055 0.56 +0.3 336,344 + nfsA HEPCGN_02055 0.79 +0.2 336,368 + nfsA HEPCGN_02055 0.83 -1.5 336,369 - nfsA HEPCGN_02055 0.83 +1.8 336,369 - nfsA HEPCGN_02055 0.83 +1.0 336,369 - nfsA HEPCGN_02055 0.83 -1.3 336,452 - -0.5 336,515 + +0.5 336,516 - +2.6 336,590 + -0.0 336,591 - +0.2 336,591 - +0.3 336,598 + -1.1 336,621 + -1.1 336,622 - -0.9 336,666 - rimK HEPCGN_02060 0.12 -1.5 336,676 - rimK HEPCGN_02060 0.14 +0.2 336,694 + rimK HEPCGN_02060 0.16 +0.3 336,694 + rimK HEPCGN_02060 0.16 +0.4 336,694 + rimK HEPCGN_02060 0.16 -0.7 336,717 + rimK HEPCGN_02060 0.18 +0.2 336,746 - rimK HEPCGN_02060 0.21 -0.9 336,761 - rimK HEPCGN_02060 0.23 +2.7 336,939 + rimK HEPCGN_02060 0.43 +1.3 336,989 - rimK HEPCGN_02060 0.48 +1.0 337,088 + rimK HEPCGN_02060 0.59 +0.4 337,088 + rimK HEPCGN_02060 0.59 +0.3 337,088 + rimK HEPCGN_02060 0.59 -0.5 337,088 + rimK HEPCGN_02060 0.59 -0.7 337,088 + rimK HEPCGN_02060 0.59 -0.1 337,089 - rimK HEPCGN_02060 0.59 +0.5 337,090 + rimK HEPCGN_02060 0.59 -0.0 337,298 - rimK HEPCGN_02060 0.82 -0.1 337,310 - rimK HEPCGN_02060 0.84 -0.5 337,363 - rimK HEPCGN_02060 0.90 -0.6 337,424 - -0.1 337,434 - +0.7 337,469 + -0.8 337,502 - +2.8 337,624 + ybjN HEPCGN_02065 0.17 +0.3 337,652 - ybjN HEPCGN_02065 0.23 +0.1 337,768 - ybjN HEPCGN_02065 0.47 +0.6 337,826 - ybjN HEPCGN_02065 0.59 +0.2 337,881 - ybjN HEPCGN_02065 0.71 -0.4 337,881 - ybjN HEPCGN_02065 0.71 -0.3 337,946 - ybjN HEPCGN_02065 0.84 +1.7 338,134 + +0.9 338,191 + +0.1 338,192 - -0.3 338,234 - -0.1 338,235 + -0.7 338,235 + +0.0 338,236 - +0.3 338,381 + -0.2 338,382 - +0.6
Or see this region's nucleotide sequence