Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF822 and GFF823 are separated by 136 nucleotides GFF823 and GFF824 are separated by 5 nucleotides GFF824 and GFF825 are separated by 164 nucleotides GFF825 and GFF826 are separated by 173 nucleotides
GFF822 - Na+/H+-dicarboxylate symporter, at 126,665 to 128,008
GFF822
GFF823 - Inner membrane protein YbaL, KefB/KefC family, at 128,145 to 129,851
GFF823
GFF824 - hypothetical protein, at 129,857 to 130,261
GFF824
GFF825 - no description, at 130,426 to 130,653
GFF825
GFF826 - no description, at 130,827 to 130,979
GFF826
Position (kb)
128
129
130 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.262 kb on + strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.263 kb on - strand, within GFF822 at 127.370 kb on + strand, within GFF822 at 127.370 kb on + strand, within GFF822 at 127.370 kb on + strand, within GFF822 at 127.370 kb on + strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.371 kb on - strand, within GFF822 at 127.374 kb on + strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.455 kb on - strand, within GFF822 at 127.584 kb on - strand, within GFF822 at 127.584 kb on - strand, within GFF822 at 127.584 kb on - strand, within GFF822 at 127.584 kb on - strand, within GFF822 at 127.584 kb on - strand, within GFF822 at 128.007 kb on - strand at 128.111 kb on + strand at 128.124 kb on + strand at 128.250 kb on + strand at 128.250 kb on + strand at 128.250 kb on + strand at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.417 kb on + strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.418 kb on - strand, within GFF823 at 129.447 kb on + strand, within GFF823 at 129.448 kb on - strand, within GFF823 at 129.501 kb on + strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.502 kb on - strand, within GFF823 at 129.976 kb on + strand, within GFF824 at 129.976 kb on + strand, within GFF824 at 129.976 kb on + strand, within GFF824 at 129.977 kb on - strand, within GFF824 at 129.977 kb on - strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.002 kb on + strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.003 kb on - strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.107 kb on + strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.108 kb on - strand, within GFF824 at 130.206 kb on + strand, within GFF824 at 130.207 kb on - strand, within GFF824 at 130.207 kb on - strand, within GFF824 at 130.319 kb on + strand at 130.319 kb on + strand at 130.319 kb on + strand at 130.319 kb on + strand at 130.320 kb on - strand at 130.320 kb on - strand at 130.320 kb on - strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.327 kb on + strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.328 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.353 kb on - strand at 130.416 kb on - strand at 130.416 kb on - strand at 130.483 kb on + strand, within GFF825 at 130.483 kb on + strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.484 kb on - strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.537 kb on + strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.538 kb on - strand, within GFF825 at 130.567 kb on + strand, within GFF825 at 130.576 kb on + strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.577 kb on - strand, within GFF825 at 130.649 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.651 kb on + strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.652 kb on - strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.784 kb on + strand at 130.785 kb on - strand at 130.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 127,262 + GFF822 0.44 +0.6 127,262 + GFF822 0.44 +0.1 127,262 + GFF822 0.44 -1.2 127,262 + GFF822 0.44 -1.6 127,262 + GFF822 0.44 +0.0 127,262 + GFF822 0.44 -0.7 127,262 + GFF822 0.44 +0.0 127,262 + GFF822 0.44 -1.3 127,262 + GFF822 0.44 -1.0 127,262 + GFF822 0.44 -0.3 127,262 + GFF822 0.44 +0.1 127,263 - GFF822 0.44 +0.2 127,263 - GFF822 0.44 -0.6 127,263 - GFF822 0.44 -0.5 127,263 - GFF822 0.44 +0.3 127,263 - GFF822 0.44 -1.9 127,263 - GFF822 0.44 -0.3 127,263 - GFF822 0.44 -0.3 127,263 - GFF822 0.44 +0.2 127,263 - GFF822 0.44 +1.1 127,263 - GFF822 0.44 -0.5 127,370 + GFF822 0.52 +0.1 127,370 + GFF822 0.52 -0.4 127,370 + GFF822 0.52 -0.8 127,370 + GFF822 0.52 -0.9 127,371 - GFF822 0.53 +0.1 127,371 - GFF822 0.53 -0.0 127,371 - GFF822 0.53 -0.7 127,371 - GFF822 0.53 -0.1 127,371 - GFF822 0.53 +1.4 127,371 - GFF822 0.53 -1.8 127,374 + GFF822 0.53 -0.3 127,455 - GFF822 0.59 +0.6 127,455 - GFF822 0.59 +0.2 127,455 - GFF822 0.59 -0.0 127,455 - GFF822 0.59 -0.2 127,455 - GFF822 0.59 -0.5 127,455 - GFF822 0.59 -0.0 127,584 - GFF822 0.68 -0.6 127,584 - GFF822 0.68 -0.6 127,584 - GFF822 0.68 +1.6 127,584 - GFF822 0.68 -0.6 127,584 - GFF822 0.68 -0.4 128,007 - -0.2 128,111 + +0.2 128,124 + -0.1 128,250 + -0.1 128,250 + +0.3 128,250 + -0.0 129,417 + GFF823 0.75 -1.2 129,417 + GFF823 0.75 +1.1 129,417 + GFF823 0.75 +0.7 129,417 + GFF823 0.75 -0.9 129,417 + GFF823 0.75 -0.6 129,417 + GFF823 0.75 -0.1 129,417 + GFF823 0.75 +0.3 129,417 + GFF823 0.75 +0.4 129,418 - GFF823 0.75 -0.9 129,418 - GFF823 0.75 -0.6 129,418 - GFF823 0.75 +0.1 129,418 - GFF823 0.75 +0.8 129,418 - GFF823 0.75 +0.4 129,418 - GFF823 0.75 +0.7 129,418 - GFF823 0.75 -2.3 129,447 + GFF823 0.76 +0.1 129,448 - GFF823 0.76 +0.6 129,501 + GFF823 0.79 -0.5 129,502 - GFF823 0.79 +1.2 129,502 - GFF823 0.79 -1.2 129,502 - GFF823 0.79 +0.5 129,502 - GFF823 0.79 +0.0 129,976 + GFF824 0.29 -0.7 129,976 + GFF824 0.29 +0.7 129,976 + GFF824 0.29 +0.2 129,977 - GFF824 0.30 +0.1 129,977 - GFF824 0.30 +0.3 130,002 + GFF824 0.36 +0.2 130,002 + GFF824 0.36 +0.3 130,002 + GFF824 0.36 -0.3 130,002 + GFF824 0.36 -0.3 130,003 - GFF824 0.36 -0.1 130,003 - GFF824 0.36 -0.6 130,003 - GFF824 0.36 -0.2 130,107 + GFF824 0.62 -0.4 130,107 + GFF824 0.62 -0.7 130,107 + GFF824 0.62 +0.3 130,107 + GFF824 0.62 -0.4 130,108 - GFF824 0.62 +0.2 130,108 - GFF824 0.62 -0.0 130,108 - GFF824 0.62 +0.3 130,108 - GFF824 0.62 -0.2 130,206 + GFF824 0.86 -1.2 130,207 - GFF824 0.86 -0.4 130,207 - GFF824 0.86 -0.6 130,319 + +1.6 130,319 + -0.9 130,319 + -2.1 130,319 + -0.7 130,320 - -0.4 130,320 - +0.5 130,320 - -0.0 130,327 + -1.4 130,327 + +1.0 130,327 + +0.6 130,327 + +0.8 130,327 + -0.2 130,327 + -1.0 130,327 + -1.2 130,327 + -0.7 130,327 + -0.6 130,327 + +0.4 130,327 + -0.1 130,327 + +0.1 130,327 + -0.4 130,328 - -0.4 130,328 - -0.2 130,328 - -0.7 130,328 - -1.7 130,328 - +0.1 130,328 - +0.9 130,328 - +0.1 130,328 - -0.6 130,328 - +0.1 130,328 - -0.7 130,328 - -0.6 130,328 - -1.0 130,328 - -1.1 130,353 - +0.3 130,353 - -0.5 130,353 - -0.8 130,353 - -0.5 130,353 - -0.1 130,416 - -0.0 130,416 - +1.2 130,483 + GFF825 0.25 -0.1 130,483 + GFF825 0.25 +0.7 130,484 - GFF825 0.25 +0.2 130,484 - GFF825 0.25 -0.1 130,484 - GFF825 0.25 +0.1 130,484 - GFF825 0.25 -1.7 130,484 - GFF825 0.25 +0.6 130,537 + GFF825 0.49 -1.0 130,537 + GFF825 0.49 -1.1 130,537 + GFF825 0.49 -0.0 130,537 + GFF825 0.49 -0.3 130,537 + GFF825 0.49 +0.3 130,537 + GFF825 0.49 -1.4 130,538 - GFF825 0.49 +0.8 130,538 - GFF825 0.49 +0.3 130,538 - GFF825 0.49 +0.5 130,538 - GFF825 0.49 -0.3 130,538 - GFF825 0.49 -2.2 130,538 - GFF825 0.49 -1.3 130,538 - GFF825 0.49 -1.6 130,538 - GFF825 0.49 -0.7 130,538 - GFF825 0.49 -0.9 130,567 + GFF825 0.62 -0.4 130,576 + GFF825 0.66 -1.7 130,577 - GFF825 0.66 +0.5 130,577 - GFF825 0.66 -0.2 130,577 - GFF825 0.66 -1.9 130,649 + -0.2 130,651 + +0.1 130,651 + -0.3 130,651 + +0.7 130,651 + -2.0 130,652 - -2.8 130,652 - -0.8 130,652 - -0.1 130,652 - -0.5 130,652 - -0.7 130,652 - +1.6 130,652 - -1.6 130,784 + +0.4 130,784 + -0.2 130,784 + -1.7 130,784 + -0.6 130,785 - +0.3 130,785 - +0.9
Or see this region's nucleotide sequence