Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7344 and GFF7345 are separated by 137 nucleotides GFF7345 and GFF7346 overlap by 4 nucleotides GFF7346 and GFF7347 are separated by 3 nucleotides
GFF7344 - Transcriptional regulator, LysR family, at 12,232 to 13,221
GFF7344
GFF7345 - Periplasmic aromatic aldehyde oxidoreductase, iron-sulfur subunit YagT, at 13,359 to 14,015
GFF7345
GFF7346 - Periplasmic aromatic aldehyde oxidoreductase, FAD binding subunit YagS, at 14,012 to 14,962
GFF7346
GFF7347 - Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR, at 14,966 to 17,161
GFF7347
Position (kb) 13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 12.556 kb on - strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.605 kb on + strand, within GFF7344 at 12.606 kb on - strand, within GFF7344 at 12.721 kb on + strand, within GFF7344 at 12.722 kb on - strand, within GFF7344 at 13.354 kb on + strand at 13.354 kb on + strand at 13.354 kb on + strand at 13.355 kb on - strand at 13.876 kb on - strand, within GFF7345 at 13.953 kb on + strand at 13.953 kb on + strand at 14.373 kb on - strand, within GFF7346 at 14.693 kb on + strand, within GFF7346 at 14.703 kb on + strand, within GFF7346 at 14.703 kb on + strand, within GFF7346 at 14.704 kb on - strand, within GFF7346 at 14.704 kb on - strand, within GFF7346
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 12,556 - GFF7344 0.33 -0.4 12,605 + GFF7344 0.38 -0.5 12,605 + GFF7344 0.38 -0.2 12,606 - GFF7344 0.38 -0.0 12,721 + GFF7344 0.49 -0.4 12,722 - GFF7344 0.49 +0.7 13,354 + +0.3 13,354 + -0.0 13,354 + -1.3 13,355 - +0.5 13,876 - GFF7345 0.79 -1.0 13,953 + +0.1 13,953 + -0.9 14,373 - GFF7346 0.38 -2.8 14,693 + GFF7346 0.72 +0.1 14,703 + GFF7346 0.73 -0.9 14,703 + GFF7346 0.73 +0.2 14,704 - GFF7346 0.73 -0.8 14,704 - GFF7346 0.73 -0.5
Or see this region's nucleotide sequence