Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7299 and GFF7300 are separated by 31 nucleotides GFF7300 and GFF7301 are separated by 124 nucleotides GFF7301 and GFF7302 are separated by 38 nucleotides
GFF7299 - MotA/TolQ/ExbB proton channel family protein, at 17,270 to 17,965
GFF7299
GFF7300 - Biopolymer transport protein ExbD/TolR, at 17,997 to 18,467
GFF7300
GFF7301 - Glycogen phosphorylase (EC 2.4.1.1), at 18,592 to 21,075
GFF7301
GFF7302 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 21,114 to 22,604
GFF7302
Position (kb) 18
19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 17.705 kb on + strand, within GFF7299 at 17.705 kb on + strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.706 kb on - strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.834 kb on + strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 17.835 kb on - strand, within GFF7299 at 18.367 kb on - strand, within GFF7300 at 18.598 kb on + strand at 18.598 kb on + strand at 18.598 kb on + strand at 18.599 kb on - strand at 18.599 kb on - strand at 18.599 kb on - strand at 18.604 kb on + strand at 18.604 kb on + strand at 18.604 kb on + strand at 18.604 kb on + strand at 18.605 kb on - strand at 18.605 kb on - strand at 18.605 kb on - strand at 18.605 kb on - strand at 18.605 kb on - strand at 18.605 kb on - strand at 18.615 kb on + strand at 18.616 kb on - strand at 18.616 kb on - strand at 18.616 kb on - strand at 18.616 kb on - strand at 18.636 kb on - strand at 18.722 kb on + strand at 18.755 kb on + strand at 18.755 kb on + strand at 18.755 kb on + strand at 18.755 kb on + strand at 18.756 kb on - strand at 18.756 kb on - strand at 18.756 kb on - strand at 18.756 kb on - strand at 18.764 kb on + strand at 18.765 kb on - strand at 18.765 kb on - strand at 18.782 kb on + strand at 18.782 kb on + strand at 18.782 kb on + strand at 18.782 kb on + strand at 18.783 kb on - strand at 18.783 kb on - strand at 18.783 kb on - strand at 18.783 kb on - strand at 18.783 kb on - strand at 18.783 kb on - strand at 18.812 kb on + strand at 18.812 kb on + strand at 18.812 kb on + strand at 18.813 kb on - strand at 18.824 kb on + strand at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.902 kb on + strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.903 kb on - strand, within GFF7301 at 18.920 kb on + strand, within GFF7301 at 19.154 kb on + strand, within GFF7301 at 19.154 kb on + strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.155 kb on - strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.259 kb on + strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.260 kb on - strand, within GFF7301 at 19.391 kb on + strand, within GFF7301 at 19.391 kb on + strand, within GFF7301 at 19.391 kb on + strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.392 kb on - strand, within GFF7301 at 19.781 kb on + strand, within GFF7301 at 19.781 kb on + strand, within GFF7301 at 19.781 kb on + strand, within GFF7301 at 19.781 kb on + strand, within GFF7301 at 19.781 kb on + strand, within GFF7301 at 19.782 kb on - strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.898 kb on + strand, within GFF7301 at 19.899 kb on - strand, within GFF7301 at 19.899 kb on - strand, within GFF7301 at 19.899 kb on - strand, within GFF7301 at 19.988 kb on + strand, within GFF7301 at 19.988 kb on + strand, within GFF7301 at 19.988 kb on + strand, within GFF7301 at 19.988 kb on + strand, within GFF7301 at 19.988 kb on + strand, within GFF7301 at 19.989 kb on - strand, within GFF7301 at 20.153 kb on + strand, within GFF7301 at 20.154 kb on - strand, within GFF7301 at 20.154 kb on - strand, within GFF7301 at 20.195 kb on + strand, within GFF7301 at 20.195 kb on + strand, within GFF7301 at 20.196 kb on - strand, within GFF7301 at 20.196 kb on - strand, within GFF7301 at 20.196 kb on - strand, within GFF7301 at 20.196 kb on - strand, within GFF7301 at 20.247 kb on - strand, within GFF7301 at 20.247 kb on - strand, within GFF7301 at 20.247 kb on - strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.300 kb on + strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.301 kb on - strand, within GFF7301 at 20.387 kb on + strand, within GFF7301 at 20.409 kb on - strand, within GFF7301 at 20.409 kb on - strand, within GFF7301 at 20.510 kb on + strand, within GFF7301 at 20.510 kb on + strand, within GFF7301 at 20.510 kb on + strand, within GFF7301 at 20.561 kb on + strand, within GFF7301 at 20.561 kb on + strand, within GFF7301 at 20.562 kb on - strand, within GFF7301 at 20.562 kb on - strand, within GFF7301 at 20.615 kb on + strand, within GFF7301 at 20.622 kb on - strand, within GFF7301 at 20.622 kb on - strand, within GFF7301 at 20.789 kb on + strand, within GFF7301 at 20.789 kb on + strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.790 kb on - strand, within GFF7301 at 20.852 kb on + strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.853 kb on - strand at 20.855 kb on + strand at 20.855 kb on + strand at 20.855 kb on + strand at 20.856 kb on - strand at 20.856 kb on - strand at 20.856 kb on - strand at 20.856 kb on - strand at 20.856 kb on - strand at 20.856 kb on - strand at 20.879 kb on + strand at 20.879 kb on + strand at 20.880 kb on - strand at 20.880 kb on - strand at 20.993 kb on + strand at 20.993 kb on + strand at 20.994 kb on - strand at 21.157 kb on + strand at 21.158 kb on - strand at 21.238 kb on + strand at 21.239 kb on - strand at 21.239 kb on - strand at 21.398 kb on - strand, within GFF7302 at 21.398 kb on - strand, within GFF7302 at 21.398 kb on - strand, within GFF7302 at 21.398 kb on - strand, within GFF7302 at 21.398 kb on - strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.409 kb on + strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.410 kb on - strand, within GFF7302 at 21.778 kb on + strand, within GFF7302 at 21.779 kb on - strand, within GFF7302 at 21.779 kb on - strand, within GFF7302 at 21.779 kb on - strand, within GFF7302 at 21.779 kb on - strand, within GFF7302 at 21.922 kb on + strand, within GFF7302 at 21.922 kb on + strand, within GFF7302 at 21.923 kb on - strand, within GFF7302 at 21.923 kb on - strand, within GFF7302 at 21.955 kb on + strand, within GFF7302 at 21.956 kb on - strand, within GFF7302 at 21.994 kb on + strand, within GFF7302 at 21.994 kb on + strand, within GFF7302 at 21.995 kb on - strand, within GFF7302 at 21.995 kb on - strand, within GFF7302 at 22.072 kb on + strand, within GFF7302 at 22.073 kb on - strand, within GFF7302 at 22.073 kb on - strand, within GFF7302
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 17,705 + GFF7299 0.62 -0.5 17,705 + GFF7299 0.62 +0.1 17,706 - GFF7299 0.63 -0.4 17,706 - GFF7299 0.63 +0.3 17,706 - GFF7299 0.63 -0.6 17,706 - GFF7299 0.63 -1.0 17,706 - GFF7299 0.63 -3.0 17,706 - GFF7299 0.63 -0.7 17,706 - GFF7299 0.63 -0.1 17,706 - GFF7299 0.63 -0.6 17,706 - GFF7299 0.63 -1.1 17,706 - GFF7299 0.63 +0.8 17,834 + GFF7299 0.81 -0.4 17,834 + GFF7299 0.81 +1.9 17,834 + GFF7299 0.81 +0.8 17,834 + GFF7299 0.81 +0.9 17,834 + GFF7299 0.81 +2.1 17,834 + GFF7299 0.81 +0.6 17,835 - GFF7299 0.81 +1.7 17,835 - GFF7299 0.81 -0.5 17,835 - GFF7299 0.81 -0.2 17,835 - GFF7299 0.81 -0.1 17,835 - GFF7299 0.81 +0.3 17,835 - GFF7299 0.81 -0.1 17,835 - GFF7299 0.81 -0.1 18,367 - GFF7300 0.79 +0.8 18,598 + +0.4 18,598 + +0.3 18,598 + +1.3 18,599 - +0.9 18,599 - -1.4 18,599 - -0.2 18,604 + -1.3 18,604 + -1.7 18,604 + -0.6 18,604 + +0.9 18,605 - +2.7 18,605 - -0.3 18,605 - +0.1 18,605 - +0.0 18,605 - -0.7 18,605 - -0.2 18,615 + -1.2 18,616 - +0.9 18,616 - +1.5 18,616 - -0.1 18,616 - -0.6 18,636 - +0.2 18,722 + -0.5 18,755 + -0.6 18,755 + -2.5 18,755 + -0.3 18,755 + -1.6 18,756 - -1.7 18,756 - -0.9 18,756 - +1.0 18,756 - -0.3 18,764 + +0.7 18,765 - +0.8 18,765 - -0.0 18,782 + -0.3 18,782 + -0.7 18,782 + +0.5 18,782 + +0.1 18,783 - +0.7 18,783 - -0.2 18,783 - +0.1 18,783 - -0.0 18,783 - -0.1 18,783 - -0.6 18,812 + -1.1 18,812 + +0.6 18,812 + -0.3 18,813 - -0.7 18,824 + -0.2 18,902 + GFF7301 0.12 +0.7 18,902 + GFF7301 0.12 -0.1 18,902 + GFF7301 0.12 -0.8 18,902 + GFF7301 0.12 -0.4 18,902 + GFF7301 0.12 -2.4 18,902 + GFF7301 0.12 -1.3 18,902 + GFF7301 0.12 -0.2 18,902 + GFF7301 0.12 -1.0 18,902 + GFF7301 0.12 -0.4 18,902 + GFF7301 0.12 -0.6 18,902 + GFF7301 0.12 -2.0 18,902 + GFF7301 0.12 -0.1 18,902 + GFF7301 0.12 -1.1 18,902 + GFF7301 0.12 -0.4 18,902 + GFF7301 0.12 +1.3 18,902 + GFF7301 0.12 +0.0 18,903 - GFF7301 0.13 -0.9 18,903 - GFF7301 0.13 -0.8 18,903 - GFF7301 0.13 +0.2 18,903 - GFF7301 0.13 -0.6 18,903 - GFF7301 0.13 -0.3 18,903 - GFF7301 0.13 -1.6 18,920 + GFF7301 0.13 +0.3 19,154 + GFF7301 0.23 -1.8 19,154 + GFF7301 0.23 -0.5 19,155 - GFF7301 0.23 -0.1 19,155 - GFF7301 0.23 -0.8 19,155 - GFF7301 0.23 +0.7 19,155 - GFF7301 0.23 -0.9 19,155 - GFF7301 0.23 -0.5 19,155 - GFF7301 0.23 -0.5 19,259 + GFF7301 0.27 -1.1 19,259 + GFF7301 0.27 +0.8 19,259 + GFF7301 0.27 +0.8 19,259 + GFF7301 0.27 -0.1 19,259 + GFF7301 0.27 -0.2 19,259 + GFF7301 0.27 +0.1 19,260 - GFF7301 0.27 -0.7 19,260 - GFF7301 0.27 -0.3 19,260 - GFF7301 0.27 -0.9 19,260 - GFF7301 0.27 -0.7 19,260 - GFF7301 0.27 +0.1 19,260 - GFF7301 0.27 +0.6 19,391 + GFF7301 0.32 -0.4 19,391 + GFF7301 0.32 -0.4 19,391 + GFF7301 0.32 +0.3 19,392 - GFF7301 0.32 +0.4 19,392 - GFF7301 0.32 +0.6 19,392 - GFF7301 0.32 -0.9 19,392 - GFF7301 0.32 -0.4 19,392 - GFF7301 0.32 -1.8 19,392 - GFF7301 0.32 +0.3 19,781 + GFF7301 0.48 -0.1 19,781 + GFF7301 0.48 -1.2 19,781 + GFF7301 0.48 -0.1 19,781 + GFF7301 0.48 -0.3 19,781 + GFF7301 0.48 +0.9 19,782 - GFF7301 0.48 -0.7 19,898 + GFF7301 0.53 -0.5 19,898 + GFF7301 0.53 +1.1 19,898 + GFF7301 0.53 +0.7 19,898 + GFF7301 0.53 +0.1 19,898 + GFF7301 0.53 -1.7 19,898 + GFF7301 0.53 -1.4 19,899 - GFF7301 0.53 +2.9 19,899 - GFF7301 0.53 +0.8 19,899 - GFF7301 0.53 +0.1 19,988 + GFF7301 0.56 +0.3 19,988 + GFF7301 0.56 -0.1 19,988 + GFF7301 0.56 -1.6 19,988 + GFF7301 0.56 +0.4 19,988 + GFF7301 0.56 -1.9 19,989 - GFF7301 0.56 +0.1 20,153 + GFF7301 0.63 -0.1 20,154 - GFF7301 0.63 -0.1 20,154 - GFF7301 0.63 -0.1 20,195 + GFF7301 0.65 -0.9 20,195 + GFF7301 0.65 +0.9 20,196 - GFF7301 0.65 +0.2 20,196 - GFF7301 0.65 -1.6 20,196 - GFF7301 0.65 -1.1 20,196 - GFF7301 0.65 -0.8 20,247 - GFF7301 0.67 -0.9 20,247 - GFF7301 0.67 -0.5 20,247 - GFF7301 0.67 -0.5 20,300 + GFF7301 0.69 +0.5 20,300 + GFF7301 0.69 -0.1 20,300 + GFF7301 0.69 -0.7 20,300 + GFF7301 0.69 -0.1 20,300 + GFF7301 0.69 +0.9 20,300 + GFF7301 0.69 -1.4 20,300 + GFF7301 0.69 +0.0 20,300 + GFF7301 0.69 -0.8 20,300 + GFF7301 0.69 +0.1 20,300 + GFF7301 0.69 +2.1 20,300 + GFF7301 0.69 -0.7 20,300 + GFF7301 0.69 +1.3 20,301 - GFF7301 0.69 +0.4 20,301 - GFF7301 0.69 -0.5 20,301 - GFF7301 0.69 -0.3 20,301 - GFF7301 0.69 +0.4 20,301 - GFF7301 0.69 +1.3 20,301 - GFF7301 0.69 +0.2 20,301 - GFF7301 0.69 -1.4 20,301 - GFF7301 0.69 +0.7 20,301 - GFF7301 0.69 -0.1 20,301 - GFF7301 0.69 -0.5 20,301 - GFF7301 0.69 -0.7 20,301 - GFF7301 0.69 +0.7 20,301 - GFF7301 0.69 -1.1 20,301 - GFF7301 0.69 -0.6 20,301 - GFF7301 0.69 +0.9 20,387 + GFF7301 0.72 -0.0 20,409 - GFF7301 0.73 +0.9 20,409 - GFF7301 0.73 -0.3 20,510 + GFF7301 0.77 +0.1 20,510 + GFF7301 0.77 +0.9 20,510 + GFF7301 0.77 +0.5 20,561 + GFF7301 0.79 -0.7 20,561 + GFF7301 0.79 -0.7 20,562 - GFF7301 0.79 +0.5 20,562 - GFF7301 0.79 -0.2 20,615 + GFF7301 0.81 -1.8 20,622 - GFF7301 0.82 -0.3 20,622 - GFF7301 0.82 +0.3 20,789 + GFF7301 0.88 +0.4 20,789 + GFF7301 0.88 -0.2 20,790 - GFF7301 0.88 +0.3 20,790 - GFF7301 0.88 -0.0 20,790 - GFF7301 0.88 -0.4 20,790 - GFF7301 0.88 -0.6 20,790 - GFF7301 0.88 -0.4 20,790 - GFF7301 0.88 +0.2 20,790 - GFF7301 0.88 -0.1 20,790 - GFF7301 0.88 +0.1 20,790 - GFF7301 0.88 +0.9 20,852 + +0.7 20,853 - -0.7 20,853 - -2.6 20,853 - +0.0 20,853 - -2.1 20,853 - -0.1 20,853 - -0.4 20,853 - -0.3 20,855 + -1.5 20,855 + -0.9 20,855 + -0.9 20,856 - -0.7 20,856 - +0.8 20,856 - +0.5 20,856 - -1.9 20,856 - -1.1 20,856 - -0.3 20,879 + -0.1 20,879 + -0.1 20,880 - -0.4 20,880 - -0.6 20,993 + -1.4 20,993 + -0.9 20,994 - -1.1 21,157 + +0.6 21,158 - -0.4 21,238 + -0.7 21,239 - +0.4 21,239 - -0.1 21,398 - GFF7302 0.19 -0.9 21,398 - GFF7302 0.19 -0.3 21,398 - GFF7302 0.19 -0.2 21,398 - GFF7302 0.19 -0.3 21,398 - GFF7302 0.19 +0.6 21,409 + GFF7302 0.20 -0.5 21,409 + GFF7302 0.20 -0.9 21,409 + GFF7302 0.20 -0.1 21,409 + GFF7302 0.20 +1.2 21,409 + GFF7302 0.20 +0.4 21,409 + GFF7302 0.20 -0.3 21,410 - GFF7302 0.20 -1.0 21,410 - GFF7302 0.20 +0.6 21,410 - GFF7302 0.20 -0.9 21,410 - GFF7302 0.20 -2.6 21,410 - GFF7302 0.20 -2.5 21,410 - GFF7302 0.20 +0.4 21,778 + GFF7302 0.45 +0.3 21,779 - GFF7302 0.45 +0.3 21,779 - GFF7302 0.45 +0.0 21,779 - GFF7302 0.45 -0.5 21,779 - GFF7302 0.45 -0.4 21,922 + GFF7302 0.54 -0.9 21,922 + GFF7302 0.54 +0.1 21,923 - GFF7302 0.54 +0.1 21,923 - GFF7302 0.54 -0.4 21,955 + GFF7302 0.56 +0.1 21,956 - GFF7302 0.56 -1.1 21,994 + GFF7302 0.59 -0.4 21,994 + GFF7302 0.59 -0.7 21,995 - GFF7302 0.59 -0.3 21,995 - GFF7302 0.59 -0.7 22,072 + GFF7302 0.64 +1.1 22,073 - GFF7302 0.64 +0.2 22,073 - GFF7302 0.64 -1.4
Or see this region's nucleotide sequence