Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7035 and GFF7036 are separated by 9 nucleotides GFF7036 and GFF7038 are separated by 57 nucleotides GFF7038 and GFF7037 overlap by 6 nucleotides GFF7037 and GFF7039 are separated by 14 nucleotides GFF7039 and GFF7040 are separated by 10 nucleotides GFF7040 and GFF7041 overlap by 4 nucleotides GFF7041 and GFF7042 are separated by 87 nucleotides
GFF7035 - UspA, at 1,387 to 1,827
GFF7035
GFF7036 - LSU rRNA pseudouridine(746) synthase (EC 5.4.99.29) @ tRNA pseudouridine(32) synthase (EC 5.4.99.28), at 1,837 to 2,508
GFF7036
GFF7038 - no description, at 2,566 to 2,721
GFF7038
GFF7037 - Uncharacterized MFS-type transporter, at 2,716 to 3,918
GFF7037
GFF7039 - Ferredoxin, at 3,933 to 4,220
GFF7039
GFF7040 - FIG00361340: hypothetical protein, at 4,231 to 4,512
GFF7040
GFF7041 - hypothetical protein, at 4,509 to 4,658
GFF7041
GFF7042 - Acetyltransferase, GNAT family, at 4,746 to 5,264
GFF7042
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1.866 kb on + strand at 1.867 kb on - strand at 1.867 kb on - strand at 1.970 kb on + strand, within GFF7036 at 1.970 kb on + strand, within GFF7036 at 1.970 kb on + strand, within GFF7036 at 2.244 kb on - strand, within GFF7036 at 2.244 kb on - strand, within GFF7036 at 2.244 kb on - strand, within GFF7036 at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.791 kb on + strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.792 kb on - strand at 2.836 kb on + strand at 2.836 kb on + strand at 2.881 kb on - strand, within GFF7037 at 2.881 kb on - strand, within GFF7037 at 3.190 kb on + strand, within GFF7037 at 3.190 kb on + strand, within GFF7037 at 3.191 kb on - strand, within GFF7037 at 3.191 kb on - strand, within GFF7037 at 3.247 kb on + strand, within GFF7037 at 3.247 kb on + strand, within GFF7037 at 3.247 kb on + strand, within GFF7037 at 3.248 kb on - strand, within GFF7037 at 3.248 kb on - strand, within GFF7037 at 3.253 kb on + strand, within GFF7037 at 3.253 kb on + strand, within GFF7037 at 3.253 kb on + strand, within GFF7037 at 3.253 kb on + strand, within GFF7037 at 3.254 kb on - strand, within GFF7037 at 3.254 kb on - strand, within GFF7037 at 3.254 kb on - strand, within GFF7037 at 3.254 kb on - strand, within GFF7037 at 3.254 kb on - strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.400 kb on + strand, within GFF7037 at 3.401 kb on - strand, within GFF7037 at 3.401 kb on - strand, within GFF7037 at 3.401 kb on - strand, within GFF7037 at 3.917 kb on - strand at 4.289 kb on + strand, within GFF7040 at 4.289 kb on + strand, within GFF7040 at 4.289 kb on + strand, within GFF7040 at 4.289 kb on + strand, within GFF7040 at 4.289 kb on + strand, within GFF7040 at 4.289 kb on + strand, within GFF7040 at 4.290 kb on - strand, within GFF7040 at 4.290 kb on - strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.412 kb on + strand, within GFF7040 at 4.413 kb on - strand, within GFF7040 at 4.413 kb on - strand, within GFF7040 at 4.667 kb on - strand at 4.677 kb on + strand at 4.678 kb on - strand at 4.717 kb on + strand at 4.718 kb on - strand at 4.830 kb on + strand, within GFF7042 at 4.830 kb on + strand, within GFF7042 at 4.831 kb on - strand, within GFF7042 at 4.831 kb on - strand, within GFF7042 at 4.911 kb on + strand, within GFF7042 at 4.911 kb on + strand, within GFF7042 at 4.911 kb on + strand, within GFF7042 at 4.912 kb on - strand, within GFF7042
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 1,866 + +0.6 1,867 - +0.4 1,867 - -0.1 1,970 + GFF7036 0.20 +0.8 1,970 + GFF7036 0.20 -0.1 1,970 + GFF7036 0.20 +0.3 2,244 - GFF7036 0.61 -0.6 2,244 - GFF7036 0.61 -0.0 2,244 - GFF7036 0.61 +0.3 2,791 + -0.7 2,791 + -0.5 2,791 + +0.3 2,791 + +0.8 2,791 + -0.9 2,791 + -0.3 2,791 + +0.7 2,791 + -1.6 2,791 + +0.2 2,791 + +1.3 2,791 + +0.4 2,791 + +1.0 2,791 + -1.6 2,792 - -1.2 2,792 - +0.2 2,792 - -0.4 2,792 - +0.1 2,792 - +0.8 2,792 - +1.0 2,792 - -0.1 2,792 - +3.4 2,792 - +1.6 2,792 - -0.3 2,792 - -0.9 2,792 - +1.0 2,792 - -1.2 2,792 - +2.2 2,792 - -1.2 2,792 - -0.9 2,792 - +0.5 2,792 - -0.2 2,792 - -0.4 2,792 - -1.4 2,792 - +0.2 2,792 - +0.7 2,792 - -0.9 2,836 + -1.4 2,836 + -0.4 2,881 - GFF7037 0.14 +0.2 2,881 - GFF7037 0.14 -0.2 3,190 + GFF7037 0.39 +0.2 3,190 + GFF7037 0.39 +0.2 3,191 - GFF7037 0.39 -0.1 3,191 - GFF7037 0.39 +1.1 3,247 + GFF7037 0.44 -0.5 3,247 + GFF7037 0.44 +0.3 3,247 + GFF7037 0.44 -0.1 3,248 - GFF7037 0.44 +0.3 3,248 - GFF7037 0.44 -0.8 3,253 + GFF7037 0.45 -1.2 3,253 + GFF7037 0.45 +0.7 3,253 + GFF7037 0.45 +0.4 3,253 + GFF7037 0.45 -0.6 3,254 - GFF7037 0.45 +2.1 3,254 - GFF7037 0.45 +0.4 3,254 - GFF7037 0.45 -0.4 3,254 - GFF7037 0.45 +0.5 3,254 - GFF7037 0.45 -0.6 3,400 + GFF7037 0.57 +0.2 3,400 + GFF7037 0.57 -0.4 3,400 + GFF7037 0.57 +0.7 3,400 + GFF7037 0.57 -1.0 3,400 + GFF7037 0.57 -0.0 3,400 + GFF7037 0.57 +0.3 3,401 - GFF7037 0.57 -0.0 3,401 - GFF7037 0.57 +1.1 3,401 - GFF7037 0.57 +0.7 3,917 - +0.1 4,289 + GFF7040 0.21 +0.7 4,289 + GFF7040 0.21 +2.0 4,289 + GFF7040 0.21 -0.4 4,289 + GFF7040 0.21 -1.4 4,289 + GFF7040 0.21 -0.4 4,289 + GFF7040 0.21 -0.5 4,290 - GFF7040 0.21 -1.4 4,290 - GFF7040 0.21 -0.2 4,412 + GFF7040 0.64 -0.8 4,412 + GFF7040 0.64 -0.4 4,412 + GFF7040 0.64 -0.4 4,412 + GFF7040 0.64 +0.3 4,412 + GFF7040 0.64 +1.4 4,412 + GFF7040 0.64 -0.8 4,413 - GFF7040 0.65 +0.2 4,413 - GFF7040 0.65 -0.6 4,667 - -0.8 4,677 + -0.5 4,678 - +1.3 4,717 + +0.2 4,718 - -0.4 4,830 + GFF7042 0.16 -2.2 4,830 + GFF7042 0.16 +0.7 4,831 - GFF7042 0.16 +0.6 4,831 - GFF7042 0.16 -1.1 4,911 + GFF7042 0.32 -0.2 4,911 + GFF7042 0.32 +0.1 4,911 + GFF7042 0.32 -0.7 4,912 - GFF7042 0.32 -1.0
Or see this region's nucleotide sequence