Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7017 and GFF7018 are separated by 12 nucleotides GFF7018 and GFF7019 overlap by 4 nucleotides GFF7019 and GFF7020 are separated by 150 nucleotides
GFF7017 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 33,364 to 34,977
GFF7017
GFF7018 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 34,990 to 35,976
GFF7018
GFF7019 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 35,973 to 36,884
GFF7019
GFF7020 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 37,035 to 38,087
GFF7020
Position (kb) 35
36
37 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 35.251 kb on + strand, within GFF7018 at 35.252 kb on - strand, within GFF7018 at 35.252 kb on - strand, within GFF7018 at 35.714 kb on - strand, within GFF7018 at 36.234 kb on + strand, within GFF7019 at 36.235 kb on - strand, within GFF7019 at 36.541 kb on - strand, within GFF7019 at 36.541 kb on - strand, within GFF7019 at 36.759 kb on + strand, within GFF7019 at 36.759 kb on + strand, within GFF7019 at 36.760 kb on - strand, within GFF7019 at 37.026 kb on + strand at 37.409 kb on - strand, within GFF7020 at 37.576 kb on + strand, within GFF7020 at 37.737 kb on + strand, within GFF7020 at 37.738 kb on - strand, within GFF7020 at 37.800 kb on + strand, within GFF7020
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 35,251 + GFF7018 0.26 -1.5 35,252 - GFF7018 0.27 -2.5 35,252 - GFF7018 0.27 -1.0 35,714 - GFF7018 0.73 -1.3 36,234 + GFF7019 0.29 -0.4 36,235 - GFF7019 0.29 -0.8 36,541 - GFF7019 0.62 -0.9 36,541 - GFF7019 0.62 -1.5 36,759 + GFF7019 0.86 -0.3 36,759 + GFF7019 0.86 -1.7 36,760 - GFF7019 0.86 -0.8 37,026 + -0.3 37,409 - GFF7020 0.36 +0.1 37,576 + GFF7020 0.51 -1.2 37,737 + GFF7020 0.67 +0.0 37,738 - GFF7020 0.67 +0.7 37,800 + GFF7020 0.73 +2.8
Or see this region's nucleotide sequence