Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7017 and GFF7018 are separated by 12 nucleotides GFF7018 and GFF7019 overlap by 4 nucleotides
GFF7017 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 33,364 to 34,977
GFF7017
GFF7018 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 34,990 to 35,976
GFF7018
GFF7019 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 35,973 to 36,884
GFF7019
Position (kb)
34
35
36 Strain fitness (log2 ratio)
-2
-1
0
1 at 34.054 kb on + strand, within GFF7017 at 34.210 kb on + strand, within GFF7017 at 34.501 kb on + strand, within GFF7017 at 34.543 kb on + strand, within GFF7017 at 34.702 kb on + strand, within GFF7017 at 34.702 kb on + strand, within GFF7017 at 34.703 kb on - strand, within GFF7017 at 34.703 kb on - strand, within GFF7017 at 34.750 kb on + strand, within GFF7017 at 34.751 kb on - strand, within GFF7017 at 34.822 kb on + strand at 34.966 kb on + strand at 34.966 kb on + strand at 34.966 kb on + strand at 34.966 kb on + strand at 34.967 kb on - strand at 34.967 kb on - strand at 34.967 kb on - strand at 35.251 kb on + strand, within GFF7018 at 35.252 kb on - strand, within GFF7018 at 35.252 kb on - strand, within GFF7018 at 35.714 kb on - strand, within GFF7018 at 36.234 kb on + strand, within GFF7019 at 36.235 kb on - strand, within GFF7019 at 36.541 kb on - strand, within GFF7019 at 36.541 kb on - strand, within GFF7019 at 36.759 kb on + strand, within GFF7019 at 36.759 kb on + strand, within GFF7019 at 36.760 kb on - strand, within GFF7019
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 34,054 + GFF7017 0.43 -0.2 34,210 + GFF7017 0.52 +0.3 34,501 + GFF7017 0.70 -0.1 34,543 + GFF7017 0.73 +0.3 34,702 + GFF7017 0.83 +0.5 34,702 + GFF7017 0.83 -0.4 34,703 - GFF7017 0.83 -1.1 34,703 - GFF7017 0.83 -0.9 34,750 + GFF7017 0.86 -1.0 34,751 - GFF7017 0.86 -0.8 34,822 + +0.2 34,966 + +0.7 34,966 + +0.1 34,966 + -1.1 34,966 + -0.2 34,967 - -0.3 34,967 - +0.2 34,967 - -0.3 35,251 + GFF7018 0.26 -1.5 35,252 - GFF7018 0.27 -2.5 35,252 - GFF7018 0.27 -1.0 35,714 - GFF7018 0.73 -1.3 36,234 + GFF7019 0.29 -0.4 36,235 - GFF7019 0.29 -0.8 36,541 - GFF7019 0.62 -0.9 36,541 - GFF7019 0.62 -1.5 36,759 + GFF7019 0.86 -0.3 36,759 + GFF7019 0.86 -1.7 36,760 - GFF7019 0.86 -0.8
Or see this region's nucleotide sequence