Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6691 and GFF6692 are separated by 425 nucleotides GFF6692 and GFF6693 overlap by 10 nucleotides GFF6693 and GFF6694 are separated by 190 nucleotides
GFF6691 - hypothetical protein, at 1 to 933
GFF6691
GFF6692 - no description, at 1,359 to 1,559
GFF6692
GFF6693 - Two-component transcriptional response regulator, LuxR family, at 1,550 to 2,320
GFF6693
GFF6694 - Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2), at 2,511 to 3,128
GFF6694
Position (kb) 1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 0.402 kb on + strand, within GFF6691 at 0.645 kb on + strand, within GFF6691 at 0.645 kb on + strand, within GFF6691 at 0.646 kb on - strand, within GFF6691 at 0.646 kb on - strand, within GFF6691 at 0.711 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.832 kb on + strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.833 kb on - strand, within GFF6691 at 0.836 kb on + strand, within GFF6691 at 0.837 kb on - strand, within GFF6691 at 0.853 kb on + strand at 0.927 kb on + strand at 0.927 kb on + strand at 0.954 kb on + strand at 0.954 kb on + strand at 0.954 kb on + strand at 0.954 kb on + strand at 0.955 kb on - strand at 0.955 kb on - strand at 0.955 kb on - strand at 0.955 kb on - strand at 0.955 kb on - strand at 1.041 kb on + strand at 1.042 kb on - strand at 1.232 kb on + strand at 1.232 kb on + strand at 1.236 kb on + strand at 1.236 kb on + strand at 1.236 kb on + strand at 1.237 kb on - strand at 1.259 kb on + strand at 1.260 kb on - strand at 1.348 kb on + strand at 1.349 kb on - strand at 1.351 kb on + strand at 1.352 kb on - strand at 1.440 kb on + strand, within GFF6692 at 1.451 kb on - strand, within GFF6692 at 1.509 kb on + strand, within GFF6692 at 1.561 kb on + strand at 1.561 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.613 kb on + strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.614 kb on - strand at 1.841 kb on + strand, within GFF6693 at 1.841 kb on + strand, within GFF6693 at 1.842 kb on - strand, within GFF6693 at 1.842 kb on - strand, within GFF6693 at 1.961 kb on + strand, within GFF6693 at 1.961 kb on + strand, within GFF6693 at 1.962 kb on - strand, within GFF6693 at 2.022 kb on + strand, within GFF6693 at 2.207 kb on + strand, within GFF6693 at 2.207 kb on + strand, within GFF6693 at 2.207 kb on + strand, within GFF6693 at 2.207 kb on + strand, within GFF6693 at 2.207 kb on + strand, within GFF6693 at 2.208 kb on - strand, within GFF6693 at 2.208 kb on - strand, within GFF6693 at 2.208 kb on - strand, within GFF6693 at 2.208 kb on - strand, within GFF6693 at 2.208 kb on - strand, within GFF6693 at 2.249 kb on + strand at 2.249 kb on + strand at 2.249 kb on + strand at 2.249 kb on + strand at 2.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 402 + GFF6691 0.43 -0.2 645 + GFF6691 0.69 -0.7 645 + GFF6691 0.69 +0.1 646 - GFF6691 0.69 -0.3 646 - GFF6691 0.69 +0.0 711 + GFF6691 0.76 -1.2 832 + GFF6691 0.89 +0.7 832 + GFF6691 0.89 -1.0 832 + GFF6691 0.89 -0.1 832 + GFF6691 0.89 -1.4 832 + GFF6691 0.89 -0.4 832 + GFF6691 0.89 -0.9 832 + GFF6691 0.89 -2.0 832 + GFF6691 0.89 -1.4 833 - GFF6691 0.89 -1.1 833 - GFF6691 0.89 +0.1 833 - GFF6691 0.89 +1.3 833 - GFF6691 0.89 +0.0 833 - GFF6691 0.89 +0.1 833 - GFF6691 0.89 +0.3 833 - GFF6691 0.89 -0.2 833 - GFF6691 0.89 -1.0 833 - GFF6691 0.89 -0.2 836 + GFF6691 0.89 -0.1 837 - GFF6691 0.90 -1.7 853 + +1.3 927 + +1.1 927 + -0.9 954 + -0.6 954 + -1.0 954 + -1.2 954 + +0.0 955 - -1.0 955 - -0.8 955 - -1.5 955 - -0.8 955 - -1.2 1,041 + +0.7 1,042 - -1.7 1,232 + -0.6 1,232 + -1.1 1,236 + -0.7 1,236 + -0.4 1,236 + -0.6 1,237 - -0.3 1,259 + -0.3 1,260 - -0.0 1,348 + -0.5 1,349 - -1.7 1,351 + -0.2 1,352 - +0.7 1,440 + GFF6692 0.40 -1.5 1,451 - GFF6692 0.46 -2.0 1,509 + GFF6692 0.75 -2.7 1,561 + +0.0 1,561 + -0.1 1,613 + +0.1 1,613 + -0.2 1,613 + +1.0 1,613 + +0.1 1,613 + +0.6 1,613 + -0.6 1,613 + -1.6 1,613 + +0.3 1,614 - -0.2 1,614 - -0.9 1,614 - -1.1 1,614 - -0.1 1,614 - +1.7 1,614 - +1.0 1,614 - +0.8 1,841 + GFF6693 0.38 -1.0 1,841 + GFF6693 0.38 -0.2 1,842 - GFF6693 0.38 +0.9 1,842 - GFF6693 0.38 -0.5 1,961 + GFF6693 0.53 +0.7 1,961 + GFF6693 0.53 -0.5 1,962 - GFF6693 0.53 -0.2 2,022 + GFF6693 0.61 +1.5 2,207 + GFF6693 0.85 -0.4 2,207 + GFF6693 0.85 +0.9 2,207 + GFF6693 0.85 -0.3 2,207 + GFF6693 0.85 -0.3 2,207 + GFF6693 0.85 +0.1 2,208 - GFF6693 0.85 -0.4 2,208 - GFF6693 0.85 -0.0 2,208 - GFF6693 0.85 +0.2 2,208 - GFF6693 0.85 +0.5 2,208 - GFF6693 0.85 -3.1 2,249 + +0.5 2,249 + -0.1 2,249 + -0.3 2,249 + +0.5 2,250 - -0.3
Or see this region's nucleotide sequence