Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6671 and GFF6672 overlap by 4 nucleotides GFF6672 and GFF6673 are separated by 221 nucleotides GFF6673 and GFF6674 are separated by 13 nucleotides GFF6674 and GFF6675 are separated by 16 nucleotides
GFF6671 - Membrane proteins related to metalloendopeptidases, at 47,398 to 48,318
GFF6671
GFF6672 - no description, at 48,315 to 48,473
GFF6672
GFF6673 - Membrane protein, at 48,695 to 49,264
GFF6673
GFF6674 - Predicted membrane protein, at 49,278 to 49,682
GFF6674
GFF6675 - ortholog of Bordetella pertussis (BX470248) BP3300, at 49,699 to 50,634
GFF6675
Position (kb) 49
50 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.336 kb on + strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.337 kb on - strand, within GFF6672 at 48.584 kb on + strand at 48.584 kb on + strand at 48.584 kb on + strand at 48.584 kb on + strand at 48.585 kb on - strand at 48.585 kb on - strand at 48.585 kb on - strand at 48.585 kb on - strand at 48.670 kb on + strand at 48.670 kb on + strand at 48.670 kb on + strand at 48.671 kb on - strand at 48.671 kb on - strand at 48.671 kb on - strand at 48.671 kb on - strand at 48.671 kb on - strand at 48.756 kb on + strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.770 kb on + strand, within GFF6673 at 48.771 kb on - strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.208 kb on + strand at 49.208 kb on + strand at 49.208 kb on + strand at 49.209 kb on - strand at 49.209 kb on - strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.263 kb on - strand at 49.263 kb on - strand at 49.307 kb on + strand at 49.308 kb on - strand at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 50.081 kb on + strand, within GFF6675 at 50.186 kb on + strand, within GFF6675 at 50.186 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.391 kb on - strand, within GFF6675 at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.556 kb on + strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.674 kb on + strand at 50.674 kb on + strand at 50.674 kb on + strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 48,336 + GFF6672 0.13 -0.3 48,336 + GFF6672 0.13 -0.4 48,336 + GFF6672 0.13 +1.9 48,336 + GFF6672 0.13 +0.2 48,336 + GFF6672 0.13 -0.2 48,336 + GFF6672 0.13 +1.1 48,336 + GFF6672 0.13 -0.8 48,336 + GFF6672 0.13 -0.1 48,336 + GFF6672 0.13 +0.3 48,336 + GFF6672 0.13 -0.1 48,337 - GFF6672 0.14 -0.4 48,337 - GFF6672 0.14 +0.1 48,337 - GFF6672 0.14 -1.9 48,337 - GFF6672 0.14 -0.1 48,337 - GFF6672 0.14 -1.2 48,337 - GFF6672 0.14 -0.2 48,337 - GFF6672 0.14 -1.6 48,337 - GFF6672 0.14 -0.1 48,337 - GFF6672 0.14 -0.9 48,337 - GFF6672 0.14 -0.8 48,337 - GFF6672 0.14 +0.4 48,337 - GFF6672 0.14 -0.3 48,337 - GFF6672 0.14 -0.7 48,584 + -0.1 48,584 + -1.2 48,584 + +0.7 48,584 + +2.6 48,585 - -0.2 48,585 - +0.5 48,585 - -1.8 48,585 - -0.7 48,670 + +2.3 48,670 + -0.3 48,670 + -0.7 48,671 - +0.4 48,671 - -0.6 48,671 - +0.6 48,671 - +0.3 48,671 - -0.3 48,756 + GFF6673 0.11 -0.6 48,757 - GFF6673 0.11 +0.3 48,757 - GFF6673 0.11 +1.1 48,757 - GFF6673 0.11 -0.2 48,770 + GFF6673 0.13 +0.4 48,771 - GFF6673 0.13 +0.6 48,965 + GFF6673 0.47 -0.0 48,965 + GFF6673 0.47 -1.0 48,965 + GFF6673 0.47 -0.4 48,965 + GFF6673 0.47 -0.2 48,965 + GFF6673 0.47 -1.6 48,965 + GFF6673 0.47 -0.4 48,965 + GFF6673 0.47 -0.0 48,965 + GFF6673 0.47 +0.8 48,965 + GFF6673 0.47 -0.1 48,965 + GFF6673 0.47 -0.4 48,965 + GFF6673 0.47 -1.3 48,965 + GFF6673 0.47 -0.3 48,965 + GFF6673 0.47 +0.6 48,965 + GFF6673 0.47 -0.0 48,965 + GFF6673 0.47 +0.1 48,966 - GFF6673 0.48 +0.4 48,966 - GFF6673 0.48 -0.3 48,966 - GFF6673 0.48 +0.2 48,966 - GFF6673 0.48 -0.8 48,966 - GFF6673 0.48 -0.4 48,966 - GFF6673 0.48 -0.3 49,106 + GFF6673 0.72 -0.8 49,106 + GFF6673 0.72 +1.3 49,106 + GFF6673 0.72 -0.8 49,106 + GFF6673 0.72 -2.4 49,106 + GFF6673 0.72 -0.2 49,107 - GFF6673 0.72 -1.2 49,107 - GFF6673 0.72 +0.1 49,107 - GFF6673 0.72 +0.6 49,107 - GFF6673 0.72 -2.2 49,107 - GFF6673 0.72 -0.3 49,107 - GFF6673 0.72 +0.1 49,107 - GFF6673 0.72 -0.3 49,118 + GFF6673 0.74 -0.0 49,118 + GFF6673 0.74 +0.8 49,118 + GFF6673 0.74 -0.5 49,208 + -0.7 49,208 + -0.5 49,208 + +0.3 49,209 - +1.2 49,209 - -1.0 49,262 + -0.5 49,262 + +0.2 49,262 + -0.5 49,263 - +1.3 49,263 - +0.1 49,307 + -0.7 49,308 - -0.4 49,369 + GFF6674 0.22 -0.6 49,369 + GFF6674 0.22 -1.4 49,369 + GFF6674 0.22 +0.5 49,369 + GFF6674 0.22 +1.5 49,370 - GFF6674 0.23 -0.4 49,370 - GFF6674 0.23 -0.3 49,370 - GFF6674 0.23 +1.6 49,408 + GFF6674 0.32 +0.4 49,408 + GFF6674 0.32 -0.7 49,408 + GFF6674 0.32 -1.4 49,408 + GFF6674 0.32 +0.8 49,408 + GFF6674 0.32 -2.2 49,408 + GFF6674 0.32 -0.9 49,408 + GFF6674 0.32 -0.6 49,409 - GFF6674 0.32 -0.4 49,409 - GFF6674 0.32 -1.1 49,409 - GFF6674 0.32 -1.4 49,409 - GFF6674 0.32 -0.5 49,409 - GFF6674 0.32 -2.3 49,409 - GFF6674 0.32 +1.1 49,409 - GFF6674 0.32 -0.2 49,409 - GFF6674 0.32 +0.8 49,409 - GFF6674 0.32 -0.2 49,409 - GFF6674 0.32 -1.4 49,718 + +0.3 49,718 + -1.7 49,718 + +0.3 49,718 + -0.0 49,718 + -1.1 49,718 + +0.9 49,718 + -0.4 49,718 + +1.3 49,718 + +0.3 49,718 + +0.9 49,718 + +0.3 49,718 + +1.2 49,719 - -1.1 49,719 - -0.0 49,719 - +0.7 49,719 - -0.3 49,719 - -0.7 49,889 + GFF6675 0.20 -0.9 49,889 + GFF6675 0.20 -0.7 49,889 + GFF6675 0.20 -0.4 49,889 + GFF6675 0.20 -0.0 49,889 + GFF6675 0.20 +0.7 49,889 + GFF6675 0.20 -0.3 49,889 + GFF6675 0.20 +0.7 49,889 + GFF6675 0.20 -0.1 49,889 + GFF6675 0.20 -0.0 49,889 + GFF6675 0.20 -0.0 49,889 + GFF6675 0.20 -0.4 49,889 + GFF6675 0.20 -0.4 49,889 + GFF6675 0.20 +0.1 49,889 + GFF6675 0.20 -0.9 49,889 + GFF6675 0.20 +0.8 49,890 - GFF6675 0.20 -0.7 49,890 - GFF6675 0.20 -0.9 49,890 - GFF6675 0.20 -0.8 49,890 - GFF6675 0.20 -0.5 49,890 - GFF6675 0.20 -0.4 49,890 - GFF6675 0.20 +0.1 49,890 - GFF6675 0.20 +0.4 49,890 - GFF6675 0.20 -0.9 49,890 - GFF6675 0.20 -0.4 49,890 - GFF6675 0.20 -0.4 49,890 - GFF6675 0.20 -0.9 49,890 - GFF6675 0.20 +0.5 49,890 - GFF6675 0.20 -0.7 49,890 - GFF6675 0.20 -0.7 49,890 - GFF6675 0.20 -1.1 49,890 - GFF6675 0.20 -0.5 49,965 - GFF6675 0.28 -0.3 49,965 - GFF6675 0.28 -0.3 49,965 - GFF6675 0.28 -1.2 49,965 - GFF6675 0.28 +1.1 49,965 - GFF6675 0.28 -0.6 49,979 + GFF6675 0.30 -0.3 49,979 + GFF6675 0.30 -0.3 49,979 + GFF6675 0.30 -0.7 49,979 + GFF6675 0.30 -0.4 49,979 + GFF6675 0.30 +1.6 49,980 - GFF6675 0.30 +1.0 49,980 - GFF6675 0.30 -0.4 49,980 - GFF6675 0.30 -3.0 49,980 - GFF6675 0.30 +0.0 49,980 - GFF6675 0.30 -0.1 49,980 - GFF6675 0.30 +0.1 50,081 + GFF6675 0.41 -0.2 50,186 + GFF6675 0.52 +0.2 50,186 + GFF6675 0.52 -0.0 50,390 + GFF6675 0.74 +0.0 50,390 + GFF6675 0.74 -0.3 50,390 + GFF6675 0.74 -2.0 50,391 - GFF6675 0.74 +1.2 50,555 + -0.4 50,555 + +0.4 50,555 + -1.2 50,555 + -1.3 50,555 + +0.3 50,555 + -0.4 50,556 + +0.8 50,556 - +0.7 50,556 - +1.0 50,556 - +1.1 50,556 - -0.0 50,556 - -0.2 50,674 + +0.6 50,674 + -2.4 50,674 + +0.6 50,675 - -0.6 50,675 - -0.6 50,675 - -0.2 50,675 - +0.0 50,675 - +0.1
Or see this region's nucleotide sequence