Strain Fitness in Variovorax sp. SCN45 around GFF6620

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
63,355	-		GFF6619	0.30	+0.8
63,355	-		GFF6619	0.30	+0.2
63,355	-		GFF6619	0.30	-0.3
63,355	-		GFF6619	0.30	+1.5
63,355	-		GFF6619	0.30	-0.8
63,396	-		GFF6619	0.34	+0.9
63,435	+		GFF6619	0.37	-1.0
63,436	-		GFF6619	0.37	+0.3
63,536	+		GFF6619	0.46	-1.1
63,536	+		GFF6619	0.46	-0.7
63,536	+		GFF6619	0.46	+0.7
63,536	+		GFF6619	0.46	+0.2
63,537	-		GFF6619	0.46	-1.2
63,537	-		GFF6619	0.46	-0.9
63,573	+		GFF6619	0.49	+1.0
63,573	+		GFF6619	0.49	-0.1
63,573	+		GFF6619	0.49	-0.3
63,573	+		GFF6619	0.49	+0.5
63,573	+		GFF6619	0.49	-1.0
63,573	+		GFF6619	0.49	+0.5
63,574	-		GFF6619	0.49	-0.0
63,574	-		GFF6619	0.49	-0.2
63,574	-		GFF6619	0.49	-0.7
63,574	-		GFF6619	0.49	-1.4
64,088	+				+0.6
64,088	+				-0.0
64,193	+				-1.1
64,193	+				+0.3
64,193	+				-1.0
64,193	+				+0.8
64,193	+				-0.9
64,193	+				+0.2
64,193	+				+0.1
64,193	+				+0.1
64,193	+				-0.3
64,193	+				-0.1
64,193	+				-1.6
64,193	+				+0.6
64,193	+				+0.3
64,194	-				+1.1
64,194	-				-0.6
64,194	-				+0.2
64,194	-				-0.6
64,194	-				-1.1
64,194	-				-0.0
64,194	-				-0.7
64,194	-				-0.8
64,194	-				-1.5
64,194	-				-0.2
64,194	-				-0.3
64,241	+				+0.2
64,241	+				+0.6
64,242	-				+0.2
64,334	+		GFF6620	0.13	-0.0
64,380	+		GFF6620	0.18	+0.1
64,380	+		GFF6620	0.18	+0.9
64,380	+		GFF6620	0.18	-0.5
64,380	+		GFF6620	0.18	+1.2
64,381	-		GFF6620	0.18	-0.3
64,396	+		GFF6620	0.20	-0.9
64,397	-		GFF6620	0.20	+0.3
64,615	-		GFF6620	0.42	-0.8
64,741	+		GFF6620	0.55	-1.4
64,741	+		GFF6620	0.55	+0.1
64,957	+		GFF6620	0.78	-0.3
64,995	+		GFF6620	0.81	+0.5
64,996	-		GFF6620	0.82	-0.2
64,996	-		GFF6620	0.82	-0.6
64,996	-		GFF6620	0.82	-0.9
65,004	+		GFF6620	0.82	+0.1
65,004	+		GFF6620	0.82	-0.3
65,101	+				-0.5
65,102	-				-1.8
65,164	+				-0.1
65,164	+				-0.2
65,165	-				+0.7
65,189	+				+0.4
65,189	+				+1.7
65,190	-				+0.5
65,197	+				-0.4
65,198	-				+0.3
65,220	+				+0.1
65,221	-				-1.7
65,221	-				+0.3
65,221	-				-0.1
65,336	+				-0.2
65,337	-				-1.6
65,376	+				+0.2
65,377	-				+0.6
65,377	-				+0.3
65,377	-				-1.1
65,377	-				+0.4
65,377	-				+0.3
65,415	-				+0.9
65,493	+				-2.2
65,493	+				-0.9
65,494	-				-0.6
65,494	-				-0.7
65,494	-				+2.5
65,507	-				-0.7
65,593	+				-1.7
65,593	+				-0.7
65,593	+				+0.3
65,593	+				-0.0
65,593	+				+0.2
65,594	-				-1.0
65,594	-				+0.3
65,648	+				+0.5
65,648	+				-0.3
65,648	+				-0.1
65,648	+				-0.1
65,648	+				-2.0
65,649	-				-1.1
65,649	-				-0.6
65,649	-				+0.3
65,649	-				-1.8
65,936	+		GFF6621	0.22	+0.9
65,936	+		GFF6621	0.22	+0.6
65,937	-		GFF6621	0.22	+0.2
65,937	-		GFF6621	0.22	-0.2
65,937	-		GFF6621	0.22	+0.2
65,937	-		GFF6621	0.22	+0.8
65,985	+		GFF6621	0.25	-0.6
65,985	+		GFF6621	0.25	+0.2
65,985	+		GFF6621	0.25	-1.1
65,985	+		GFF6621	0.25	-0.9
65,986	-		GFF6621	0.25	-0.4
65,986	-		GFF6621	0.25	+1.8
65,986	-		GFF6621	0.25	-0.2
66,122	+		GFF6621	0.34	-0.2
66,122	+		GFF6621	0.34	+0.0
66,122	+		GFF6621	0.34	-0.8
66,122	+		GFF6621	0.34	-0.4
66,123	-		GFF6621	0.34	-2.6
66,123	-		GFF6621	0.34	-0.8
66,123	-		GFF6621	0.34	+1.3
66,123	-		GFF6621	0.34	-1.1