Strain Fitness in Variovorax sp. SCN45 around GFF6548

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6546 and GFF6547 are separated by 51 nucleotidesGFF6547 and GFF6548 are separated by 72 nucleotidesGFF6548 and GFF6549 are separated by 22 nucleotidesGFF6549 and GFF6550 are separated by 163 nucleotides GFF6546 - hypothetical protein, at 44,152 to 45,111 GFF6546 GFF6547 - Glutathione S-transferase (EC 2.5.1.18), at 45,163 to 45,867 GFF6547 GFF6548 - hypothetical protein, at 45,940 to 46,593 GFF6548 GFF6549 - no description, at 46,616 to 47,392 GFF6549 GFF6550 - 4'-phosphopantetheinyl transferase (EC 2.7.8.-), at 47,556 to 48,371 GFF6550 Position (kb) 45 46 47Strain fitness (log2 ratio) -2 -1 0 1 2at 44.965 kb on + strand, within GFF6546at 44.965 kb on + strand, within GFF6546at 44.966 kb on - strand, within GFF6546at 45.232 kb on + strandat 45.232 kb on + strandat 45.232 kb on + strandat 45.232 kb on + strandat 45.233 kb on - strandat 45.233 kb on - strandat 45.296 kb on - strand, within GFF6547at 45.448 kb on + strand, within GFF6547at 45.448 kb on + strand, within GFF6547at 45.449 kb on - strand, within GFF6547at 45.449 kb on - strand, within GFF6547at 45.449 kb on - strand, within GFF6547at 45.592 kb on + strand, within GFF6547at 45.592 kb on + strand, within GFF6547at 45.593 kb on - strand, within GFF6547at 45.593 kb on - strand, within GFF6547at 45.619 kb on + strand, within GFF6547at 45.619 kb on + strand, within GFF6547at 45.620 kb on - strand, within GFF6547at 45.697 kb on + strand, within GFF6547at 45.697 kb on + strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.698 kb on - strand, within GFF6547at 45.751 kb on + strand, within GFF6547at 45.751 kb on + strand, within GFF6547at 45.751 kb on + strand, within GFF6547at 45.751 kb on + strand, within GFF6547at 46.060 kb on + strand, within GFF6548at 46.228 kb on + strand, within GFF6548at 46.229 kb on - strand, within GFF6548at 46.229 kb on - strand, within GFF6548at 46.240 kb on + strand, within GFF6548at 46.240 kb on + strand, within GFF6548at 46.240 kb on + strand, within GFF6548at 46.240 kb on + strand, within GFF6548at 46.240 kb on + strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.241 kb on - strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.348 kb on + strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.349 kb on - strand, within GFF6548at 46.351 kb on + strand, within GFF6548at 46.351 kb on + strand, within GFF6548at 46.351 kb on + strand, within GFF6548at 46.351 kb on + strand, within GFF6548at 46.352 kb on - strand, within GFF6548at 46.352 kb on - strand, within GFF6548at 46.352 kb on - strand, within GFF6548at 46.352 kb on - strand, within GFF6548at 46.352 kb on - strand, within GFF6548at 46.647 kb on + strandat 46.648 kb on - strandat 46.648 kb on - strandat 46.740 kb on + strand, within GFF6549at 46.740 kb on + strand, within GFF6549at 46.740 kb on + strand, within GFF6549at 46.740 kb on + strand, within GFF6549at 46.741 kb on - strand, within GFF6549at 46.741 kb on - strand, within GFF6549at 46.741 kb on - strand, within GFF6549at 46.741 kb on - strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.139 kb on + strand, within GFF6549at 47.140 kb on - strand, within GFF6549at 47.140 kb on - strand, within GFF6549at 47.432 kb on + strandat 47.441 kb on + strandat 47.441 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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44,965 + GFF6546 0.85 +0.1
44,965 + GFF6546 0.85 +1.0
44,966 - GFF6546 0.85 +0.1
45,232 + -0.4
45,232 + -0.8
45,232 + -0.2
45,232 + -0.3
45,233 - -1.2
45,233 - +1.7
45,296 - GFF6547 0.19 +0.6
45,448 + GFF6547 0.40 +0.1
45,448 + GFF6547 0.40 +1.1
45,449 - GFF6547 0.41 -0.5
45,449 - GFF6547 0.41 -0.6
45,449 - GFF6547 0.41 +0.6
45,592 + GFF6547 0.61 -0.8
45,592 + GFF6547 0.61 +0.5
45,593 - GFF6547 0.61 +0.6
45,593 - GFF6547 0.61 +0.1
45,619 + GFF6547 0.65 -0.2
45,619 + GFF6547 0.65 -0.1
45,620 - GFF6547 0.65 -0.0
45,697 + GFF6547 0.76 +0.1
45,697 + GFF6547 0.76 +0.3
45,698 - GFF6547 0.76 -1.4
45,698 - GFF6547 0.76 -0.5
45,698 - GFF6547 0.76 +0.6
45,698 - GFF6547 0.76 -0.5
45,698 - GFF6547 0.76 +0.3
45,698 - GFF6547 0.76 +1.1
45,751 + GFF6547 0.83 +0.1
45,751 + GFF6547 0.83 +0.9
45,751 + GFF6547 0.83 -2.0
45,751 + GFF6547 0.83 -0.3
46,060 + GFF6548 0.18 +1.0
46,228 + GFF6548 0.44 +0.3
46,229 - GFF6548 0.44 +0.6
46,229 - GFF6548 0.44 -0.1
46,240 + GFF6548 0.46 -1.9
46,240 + GFF6548 0.46 -0.4
46,240 + GFF6548 0.46 -1.1
46,240 + GFF6548 0.46 -0.6
46,240 + GFF6548 0.46 -2.7
46,241 - GFF6548 0.46 -0.3
46,241 - GFF6548 0.46 -0.6
46,241 - GFF6548 0.46 -2.6
46,241 - GFF6548 0.46 -0.5
46,241 - GFF6548 0.46 -0.3
46,241 - GFF6548 0.46 -0.7
46,348 + GFF6548 0.62 +1.3
46,348 + GFF6548 0.62 -0.4
46,348 + GFF6548 0.62 +0.6
46,348 + GFF6548 0.62 -0.8
46,348 + GFF6548 0.62 +0.6
46,348 + GFF6548 0.62 -0.6
46,348 + GFF6548 0.62 -2.1
46,349 - GFF6548 0.63 -1.0
46,349 - GFF6548 0.63 -0.7
46,349 - GFF6548 0.63 +0.8
46,349 - GFF6548 0.63 -0.1
46,349 - GFF6548 0.63 -0.7
46,349 - GFF6548 0.63 +0.3
46,349 - GFF6548 0.63 -0.2
46,349 - GFF6548 0.63 +0.2
46,349 - GFF6548 0.63 -0.8
46,349 - GFF6548 0.63 -1.5
46,349 - GFF6548 0.63 -2.3
46,349 - GFF6548 0.63 +0.2
46,349 - GFF6548 0.63 -0.1
46,351 + GFF6548 0.63 -0.8
46,351 + GFF6548 0.63 +0.0
46,351 + GFF6548 0.63 -0.5
46,351 + GFF6548 0.63 -0.9
46,352 - GFF6548 0.63 -1.0
46,352 - GFF6548 0.63 +0.6
46,352 - GFF6548 0.63 -1.3
46,352 - GFF6548 0.63 -0.1
46,352 - GFF6548 0.63 +0.4
46,647 + -1.0
46,648 - -0.0
46,648 - +2.0
46,740 + GFF6549 0.16 -0.2
46,740 + GFF6549 0.16 +0.4
46,740 + GFF6549 0.16 +0.1
46,740 + GFF6549 0.16 -1.0
46,741 - GFF6549 0.16 +0.6
46,741 - GFF6549 0.16 +1.1
46,741 - GFF6549 0.16 -0.2
46,741 - GFF6549 0.16 -0.0
47,139 + GFF6549 0.67 -1.0
47,139 + GFF6549 0.67 +1.4
47,139 + GFF6549 0.67 -1.1
47,139 + GFF6549 0.67 -0.1
47,139 + GFF6549 0.67 -0.5
47,139 + GFF6549 0.67 -1.4
47,140 - GFF6549 0.67 -1.6
47,140 - GFF6549 0.67 -0.2
47,432 + +0.1
47,441 + -0.7
47,441 + -1.0

Or see this region's nucleotide sequence