Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6278 and GFF6279 overlap by 4 nucleotides GFF6279 and GFF6280 are separated by 176 nucleotides GFF6280 and GFF6281 are separated by 118 nucleotides
GFF6278 - Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), at 67,827 to 69,557
GFF6278
GFF6279 - hypothetical protein, at 69,554 to 70,471
GFF6279
GFF6280 - FIG00348512: hypothetical protein, at 70,648 to 71,076
GFF6280
GFF6281 - hypothetical protein, at 71,195 to 71,698
GFF6281
Position (kb) 69
70
71 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 69.269 kb on + strand, within GFF6278 at 69.269 kb on + strand, within GFF6278 at 69.270 kb on - strand, within GFF6278 at 69.270 kb on - strand, within GFF6278 at 69.270 kb on - strand, within GFF6278 at 69.270 kb on - strand, within GFF6278 at 69.471 kb on + strand at 69.471 kb on + strand at 69.471 kb on + strand at 69.471 kb on + strand at 69.471 kb on + strand at 69.471 kb on + strand at 69.471 kb on + strand at 69.472 kb on - strand at 69.472 kb on - strand at 69.472 kb on - strand at 69.472 kb on - strand at 69.472 kb on - strand at 69.472 kb on - strand at 69.662 kb on + strand, within GFF6279 at 69.662 kb on + strand, within GFF6279 at 69.779 kb on + strand, within GFF6279 at 69.780 kb on - strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.151 kb on + strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.152 kb on - strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.223 kb on + strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.224 kb on - strand, within GFF6279 at 70.396 kb on + strand at 70.396 kb on + strand at 70.396 kb on + strand at 70.396 kb on + strand at 70.396 kb on + strand at 70.396 kb on + strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.397 kb on - strand at 70.458 kb on + strand at 70.458 kb on + strand at 70.459 kb on - strand at 70.459 kb on - strand at 70.459 kb on - strand at 70.459 kb on - strand at 70.469 kb on + strand at 70.469 kb on + strand at 70.470 kb on - strand at 70.470 kb on - strand at 70.470 kb on - strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.540 kb on + strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.541 kb on - strand at 70.542 kb on + strand at 70.542 kb on + strand at 70.543 kb on - strand at 70.543 kb on - strand at 70.543 kb on - strand at 70.543 kb on - strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.559 kb on + strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.560 kb on - strand at 70.604 kb on + strand at 70.604 kb on + strand at 70.604 kb on + strand at 70.604 kb on + strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.605 kb on - strand at 70.624 kb on + strand at 70.624 kb on + strand at 70.624 kb on + strand at 70.625 kb on - strand at 70.625 kb on - strand at 70.625 kb on - strand at 70.625 kb on - strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.633 kb on + strand at 70.634 kb on - strand at 70.634 kb on - strand at 70.634 kb on - strand at 70.634 kb on - strand at 70.908 kb on - strand, within GFF6280 at 71.093 kb on - strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.206 kb on - strand at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.297 kb on + strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.298 kb on - strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.456 kb on + strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.457 kb on - strand, within GFF6281 at 71.471 kb on + strand, within GFF6281
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 69,269 + GFF6278 0.83 -0.2 69,269 + GFF6278 0.83 -0.4 69,270 - GFF6278 0.83 +0.3 69,270 - GFF6278 0.83 -0.4 69,270 - GFF6278 0.83 -0.9 69,270 - GFF6278 0.83 +0.1 69,471 + -0.5 69,471 + -1.2 69,471 + -0.5 69,471 + +0.9 69,471 + -1.3 69,471 + -0.1 69,471 + -0.5 69,472 - +0.0 69,472 - -0.8 69,472 - -0.3 69,472 - -0.6 69,472 - -2.6 69,472 - +1.9 69,662 + GFF6279 0.12 -1.0 69,662 + GFF6279 0.12 -0.1 69,779 + GFF6279 0.25 +0.1 69,780 - GFF6279 0.25 -1.8 70,151 + GFF6279 0.65 -0.1 70,151 + GFF6279 0.65 -0.3 70,151 + GFF6279 0.65 -1.1 70,151 + GFF6279 0.65 -1.8 70,151 + GFF6279 0.65 -0.9 70,151 + GFF6279 0.65 -0.3 70,152 - GFF6279 0.65 -1.3 70,152 - GFF6279 0.65 +0.3 70,152 - GFF6279 0.65 -1.1 70,152 - GFF6279 0.65 -0.6 70,152 - GFF6279 0.65 -0.5 70,152 - GFF6279 0.65 -0.3 70,223 + GFF6279 0.73 -0.1 70,223 + GFF6279 0.73 -1.0 70,223 + GFF6279 0.73 +0.2 70,223 + GFF6279 0.73 -1.3 70,223 + GFF6279 0.73 +0.1 70,223 + GFF6279 0.73 -1.1 70,223 + GFF6279 0.73 -0.2 70,223 + GFF6279 0.73 -1.1 70,223 + GFF6279 0.73 +0.9 70,223 + GFF6279 0.73 -0.7 70,223 + GFF6279 0.73 -1.0 70,223 + GFF6279 0.73 -0.1 70,223 + GFF6279 0.73 -1.3 70,224 - GFF6279 0.73 -2.0 70,224 - GFF6279 0.73 -1.8 70,224 - GFF6279 0.73 -1.5 70,224 - GFF6279 0.73 -1.5 70,224 - GFF6279 0.73 +0.1 70,224 - GFF6279 0.73 +1.5 70,224 - GFF6279 0.73 -0.8 70,224 - GFF6279 0.73 -0.5 70,224 - GFF6279 0.73 +0.5 70,396 + -0.3 70,396 + -0.8 70,396 + -0.0 70,396 + +0.1 70,396 + -1.1 70,396 + +0.3 70,397 - -0.2 70,397 - -0.7 70,397 - -1.7 70,397 - +0.3 70,397 - -1.7 70,397 - -1.3 70,397 - -0.3 70,458 + -1.1 70,458 + +1.9 70,459 - -0.1 70,459 - -0.9 70,459 - -0.8 70,459 - -1.3 70,469 + +0.3 70,469 + -0.5 70,470 - -0.4 70,470 - -0.2 70,470 - +0.8 70,540 + -0.3 70,540 + -0.9 70,540 + -0.6 70,540 + -0.0 70,540 + -0.3 70,540 + +1.2 70,540 + -0.0 70,540 + -0.5 70,540 + -0.6 70,540 + -0.3 70,540 + -0.9 70,540 + -0.1 70,540 + -0.7 70,540 + -0.0 70,540 + -0.4 70,540 + -0.5 70,540 + +0.6 70,540 + -0.1 70,541 - -0.1 70,541 - +1.9 70,541 - -0.1 70,541 - +1.6 70,541 - -1.0 70,541 - +0.6 70,541 - -0.6 70,541 - -0.2 70,541 - -0.4 70,541 - -0.8 70,541 - -0.9 70,541 - -0.1 70,541 - +0.0 70,541 - -0.2 70,541 - -0.5 70,541 - -0.2 70,541 - -0.6 70,541 - -0.3 70,541 - +1.3 70,541 - -0.1 70,541 - -0.4 70,542 + +1.1 70,542 + -2.5 70,543 - -0.7 70,543 - -1.4 70,543 - -0.6 70,543 - -1.0 70,559 + -0.1 70,559 + -0.2 70,559 + -0.2 70,559 + +1.0 70,559 + +0.3 70,559 + +0.7 70,559 + -0.1 70,559 + -0.4 70,559 + -0.6 70,559 + +0.3 70,559 + +0.5 70,559 + -0.7 70,559 + +0.2 70,559 + -0.1 70,559 + +0.2 70,559 + +1.4 70,560 - -0.0 70,560 - -0.1 70,560 - +0.2 70,560 - -0.4 70,560 - +0.1 70,560 - -0.3 70,560 - -0.3 70,560 - +0.3 70,560 - -1.3 70,560 - -1.3 70,560 - -0.4 70,560 - +0.5 70,604 + -0.5 70,604 + +0.2 70,604 + +0.4 70,604 + +1.1 70,605 - +0.9 70,605 - +0.8 70,605 - +1.4 70,605 - +0.1 70,605 - +0.3 70,605 - -0.2 70,605 - -1.1 70,605 - +0.6 70,624 + -0.9 70,624 + +0.5 70,624 + -1.0 70,625 - +0.7 70,625 - -0.3 70,625 - +0.6 70,625 - -0.1 70,633 + +0.6 70,633 + -0.3 70,633 + +0.0 70,633 + +0.4 70,633 + -0.3 70,633 + +0.9 70,633 + +0.9 70,634 - -0.8 70,634 - +0.3 70,634 - -1.0 70,634 - -1.0 70,908 - GFF6280 0.61 +1.0 71,093 - -0.3 71,166 + -0.2 71,166 + +0.0 71,166 + -0.3 71,166 + -1.7 71,166 + -0.6 71,166 + +0.5 71,166 + +0.3 71,166 + -0.6 71,166 + +1.1 71,166 + -0.1 71,167 - -0.9 71,167 - +0.1 71,167 - -1.1 71,167 - -0.1 71,167 - -0.9 71,167 - -0.4 71,167 - +2.3 71,167 - -0.5 71,167 - +0.5 71,206 - +0.1 71,297 + GFF6281 0.20 +0.9 71,297 + GFF6281 0.20 -0.9 71,297 + GFF6281 0.20 +0.0 71,297 + GFF6281 0.20 -0.5 71,297 + GFF6281 0.20 -0.5 71,297 + GFF6281 0.20 -0.2 71,297 + GFF6281 0.20 -0.1 71,297 + GFF6281 0.20 +0.1 71,297 + GFF6281 0.20 +0.9 71,297 + GFF6281 0.20 +0.1 71,297 + GFF6281 0.20 -0.9 71,297 + GFF6281 0.20 -0.1 71,297 + GFF6281 0.20 +0.3 71,297 + GFF6281 0.20 +0.1 71,297 + GFF6281 0.20 +0.3 71,297 + GFF6281 0.20 +0.1 71,297 + GFF6281 0.20 -2.1 71,297 + GFF6281 0.20 +0.4 71,297 + GFF6281 0.20 -1.3 71,297 + GFF6281 0.20 -0.7 71,297 + GFF6281 0.20 +0.6 71,297 + GFF6281 0.20 -1.5 71,297 + GFF6281 0.20 -0.6 71,297 + GFF6281 0.20 -0.7 71,297 + GFF6281 0.20 +0.4 71,297 + GFF6281 0.20 -0.3 71,297 + GFF6281 0.20 +0.3 71,297 + GFF6281 0.20 -0.2 71,297 + GFF6281 0.20 +0.9 71,297 + GFF6281 0.20 +0.2 71,297 + GFF6281 0.20 -0.7 71,297 + GFF6281 0.20 +0.4 71,297 + GFF6281 0.20 -0.7 71,297 + GFF6281 0.20 -1.5 71,297 + GFF6281 0.20 +3.9 71,297 + GFF6281 0.20 +0.1 71,298 - GFF6281 0.20 +0.1 71,298 - GFF6281 0.20 +1.3 71,298 - GFF6281 0.20 -0.6 71,298 - GFF6281 0.20 -0.0 71,298 - GFF6281 0.20 -1.9 71,298 - GFF6281 0.20 -1.7 71,298 - GFF6281 0.20 -1.6 71,298 - GFF6281 0.20 +0.1 71,298 - GFF6281 0.20 +0.4 71,298 - GFF6281 0.20 -0.8 71,298 - GFF6281 0.20 +1.1 71,298 - GFF6281 0.20 -0.7 71,298 - GFF6281 0.20 -1.4 71,298 - GFF6281 0.20 -1.2 71,298 - GFF6281 0.20 -0.7 71,298 - GFF6281 0.20 -0.7 71,298 - GFF6281 0.20 -0.0 71,298 - GFF6281 0.20 +0.3 71,298 - GFF6281 0.20 -1.8 71,298 - GFF6281 0.20 -0.8 71,298 - GFF6281 0.20 +0.5 71,298 - GFF6281 0.20 +0.3 71,298 - GFF6281 0.20 -0.4 71,298 - GFF6281 0.20 +0.0 71,298 - GFF6281 0.20 +0.9 71,298 - GFF6281 0.20 +0.8 71,298 - GFF6281 0.20 -0.6 71,456 + GFF6281 0.52 -1.2 71,456 + GFF6281 0.52 -0.1 71,456 + GFF6281 0.52 +0.2 71,456 + GFF6281 0.52 +0.5 71,456 + GFF6281 0.52 -2.4 71,456 + GFF6281 0.52 +0.2 71,456 + GFF6281 0.52 +0.1 71,456 + GFF6281 0.52 +0.2 71,456 + GFF6281 0.52 -0.5 71,456 + GFF6281 0.52 +0.3 71,456 + GFF6281 0.52 -0.1 71,456 + GFF6281 0.52 +1.1 71,456 + GFF6281 0.52 -2.3 71,457 - GFF6281 0.52 -0.1 71,457 - GFF6281 0.52 -0.6 71,457 - GFF6281 0.52 -0.1 71,457 - GFF6281 0.52 -0.4 71,457 - GFF6281 0.52 +1.4 71,457 - GFF6281 0.52 -0.2 71,457 - GFF6281 0.52 +1.1 71,457 - GFF6281 0.52 +0.5 71,471 + GFF6281 0.55 -0.7
Or see this region's nucleotide sequence