Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6217 and GFF6218 overlap by 11 nucleotides
GFF6217 - hypothetical protein, at 125,245 to 126,237
GFF6217
GFF6218 - no description, at 126,227 to 126,571
GFF6218
Position (kb)
126
127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 125.265 kb on + strand at 125.265 kb on + strand at 125.265 kb on + strand at 125.265 kb on + strand at 125.424 kb on + strand, within GFF6217 at 125.424 kb on + strand, within GFF6217 at 125.425 kb on - strand, within GFF6217 at 125.673 kb on + strand, within GFF6217 at 125.673 kb on + strand, within GFF6217 at 125.673 kb on + strand, within GFF6217 at 125.673 kb on + strand, within GFF6217 at 125.674 kb on - strand, within GFF6217 at 125.694 kb on + strand, within GFF6217 at 125.695 kb on - strand, within GFF6217 at 125.695 kb on - strand, within GFF6217 at 125.850 kb on - strand, within GFF6217 at 125.904 kb on + strand, within GFF6217 at 125.904 kb on + strand, within GFF6217 at 125.905 kb on - strand, within GFF6217 at 125.936 kb on - strand, within GFF6217 at 125.936 kb on - strand, within GFF6217 at 125.969 kb on - strand, within GFF6217 at 126.004 kb on + strand, within GFF6217 at 126.035 kb on + strand, within GFF6217 at 126.118 kb on + strand, within GFF6217 at 126.118 kb on + strand, within GFF6217 at 126.119 kb on - strand, within GFF6217 at 126.119 kb on - strand, within GFF6217 at 126.229 kb on - strand at 126.283 kb on - strand, within GFF6218 at 126.283 kb on - strand, within GFF6218 at 126.283 kb on - strand, within GFF6218 at 126.283 kb on - strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.312 kb on + strand, within GFF6218 at 126.313 kb on - strand, within GFF6218 at 126.313 kb on - strand, within GFF6218 at 126.313 kb on - strand, within GFF6218 at 126.357 kb on + strand, within GFF6218 at 126.357 kb on + strand, within GFF6218 at 126.357 kb on + strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.358 kb on - strand, within GFF6218 at 126.591 kb on - strand at 126.591 kb on - strand at 126.835 kb on + strand at 126.835 kb on + strand at 126.850 kb on + strand at 126.851 kb on - strand at 126.851 kb on - strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.103 kb on - strand at 127.103 kb on - strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 125,265 + +0.4 125,265 + +0.6 125,265 + +0.2 125,265 + -0.7 125,424 + GFF6217 0.18 -0.2 125,424 + GFF6217 0.18 -0.2 125,425 - GFF6217 0.18 -0.6 125,673 + GFF6217 0.43 +0.1 125,673 + GFF6217 0.43 -1.4 125,673 + GFF6217 0.43 -0.6 125,673 + GFF6217 0.43 +0.2 125,674 - GFF6217 0.43 -1.0 125,694 + GFF6217 0.45 +0.6 125,695 - GFF6217 0.45 -1.2 125,695 - GFF6217 0.45 -2.1 125,850 - GFF6217 0.61 +0.5 125,904 + GFF6217 0.66 +0.5 125,904 + GFF6217 0.66 -0.0 125,905 - GFF6217 0.66 +0.1 125,936 - GFF6217 0.70 +0.4 125,936 - GFF6217 0.70 -0.2 125,969 - GFF6217 0.73 +1.1 126,004 + GFF6217 0.76 -0.1 126,035 + GFF6217 0.80 +0.5 126,118 + GFF6217 0.88 -0.5 126,118 + GFF6217 0.88 +0.1 126,119 - GFF6217 0.88 +0.6 126,119 - GFF6217 0.88 -0.5 126,229 - +0.0 126,283 - GFF6218 0.16 +0.1 126,283 - GFF6218 0.16 +0.3 126,283 - GFF6218 0.16 +0.5 126,283 - GFF6218 0.16 -0.7 126,312 + GFF6218 0.25 -0.5 126,312 + GFF6218 0.25 -0.2 126,312 + GFF6218 0.25 +0.0 126,312 + GFF6218 0.25 -0.1 126,312 + GFF6218 0.25 +1.0 126,312 + GFF6218 0.25 +0.9 126,313 - GFF6218 0.25 -0.1 126,313 - GFF6218 0.25 +0.4 126,313 - GFF6218 0.25 +1.0 126,357 + GFF6218 0.38 -0.4 126,357 + GFF6218 0.38 -0.9 126,357 + GFF6218 0.38 -0.8 126,358 - GFF6218 0.38 -0.2 126,358 - GFF6218 0.38 -0.9 126,358 - GFF6218 0.38 -0.4 126,358 - GFF6218 0.38 -0.4 126,358 - GFF6218 0.38 +1.0 126,358 - GFF6218 0.38 +0.1 126,358 - GFF6218 0.38 +1.1 126,591 - -0.9 126,591 - -0.4 126,835 + +0.8 126,835 + -0.2 126,850 + -1.2 126,851 - +0.4 126,851 - -0.9 126,920 + -0.2 126,920 + +0.3 126,920 + -0.2 126,920 + +0.2 126,920 + -0.2 126,920 + -0.2 126,920 + +0.1 126,920 + -1.0 126,920 + +0.9 126,921 - -0.2 126,921 - +0.2 126,921 - -0.9 126,921 - +0.2 126,921 - +0.3 126,921 - -0.3 126,921 - -0.2 126,921 - -1.1 126,921 - -1.6 126,921 - -0.0 126,921 - -0.5 126,921 - -0.1 126,955 + -0.5 126,955 + +0.1 126,955 + +0.2 126,955 + -0.9 126,955 + -0.7 126,955 + -0.2 126,955 + +0.6 126,956 - -0.5 126,956 - +0.1 126,956 - +0.4 126,956 - -0.1 126,956 - +2.4 127,102 + -0.5 127,102 + +1.5 127,102 + -0.6 127,102 + -0.1 127,103 - -0.6 127,103 - -0.1 127,166 + +0.6 127,166 + -0.3 127,166 + -1.2 127,166 + +0.1 127,166 + -0.5 127,167 - -0.4 127,167 - +0.3 127,167 - -1.2 127,167 - -0.7 127,412 + -0.8 127,412 + +1.3 127,412 + -0.3 127,412 + -0.2 127,412 + +1.0 127,412 + -0.7 127,412 + -0.4 127,412 + -0.6 127,413 - -0.2 127,413 - -0.0 127,413 - -0.1
Or see this region's nucleotide sequence