Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6165 and GFF6166 are separated by 49 nucleotides GFF6166 and GFF6167 are separated by 109 nucleotides GFF6167 and GFF6168 are separated by 22 nucleotides GFF6168 and GFF6169 overlap by 4 nucleotides GFF6169 and GFF6170 overlap by 4 nucleotides
GFF6165 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 70,737 to 71,603
GFF6165
GFF6166 - Transcriptional regulator, AcrR family, at 71,653 to 72,312
GFF6166
GFF6167 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 72,422 to 73,426
GFF6167
GFF6168 - Na(+) H(+) antiporter subunit G, at 73,449 to 73,820
GFF6168
GFF6169 - Na(+) H(+) antiporter subunit F, at 73,817 to 74,095
GFF6169
GFF6170 - Na(+) H(+) antiporter subunit E, at 74,092 to 74,583
GFF6170
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 71.567 kb on - strand at 71.582 kb on - strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.604 kb on - strand at 71.604 kb on - strand at 71.604 kb on - strand at 71.778 kb on - strand, within GFF6166 at 71.778 kb on - strand, within GFF6166 at 71.801 kb on + strand, within GFF6166 at 71.801 kb on + strand, within GFF6166 at 71.801 kb on + strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.802 kb on - strand, within GFF6166 at 71.987 kb on + strand, within GFF6166 at 71.988 kb on - strand, within GFF6166 at 72.080 kb on + strand, within GFF6166 at 72.081 kb on - strand, within GFF6166 at 72.104 kb on + strand, within GFF6166 at 72.104 kb on + strand, within GFF6166 at 72.105 kb on - strand, within GFF6166 at 72.105 kb on - strand, within GFF6166 at 72.105 kb on - strand, within GFF6166 at 72.105 kb on - strand, within GFF6166 at 72.128 kb on + strand, within GFF6166 at 72.128 kb on + strand, within GFF6166 at 72.129 kb on - strand, within GFF6166 at 72.129 kb on - strand, within GFF6166 at 72.129 kb on - strand, within GFF6166 at 72.129 kb on - strand, within GFF6166 at 72.134 kb on + strand, within GFF6166 at 72.135 kb on - strand, within GFF6166 at 72.135 kb on - strand, within GFF6166 at 72.375 kb on - strand at 72.558 kb on - strand, within GFF6167 at 72.558 kb on - strand, within GFF6167 at 72.558 kb on - strand, within GFF6167 at 72.560 kb on + strand, within GFF6167 at 72.560 kb on + strand, within GFF6167 at 72.560 kb on + strand, within GFF6167 at 72.561 kb on - strand, within GFF6167 at 72.561 kb on - strand, within GFF6167 at 72.582 kb on - strand, within GFF6167 at 72.743 kb on + strand, within GFF6167 at 72.767 kb on + strand, within GFF6167 at 72.767 kb on + strand, within GFF6167 at 72.767 kb on + strand, within GFF6167 at 72.767 kb on + strand, within GFF6167 at 72.767 kb on + strand, within GFF6167 at 72.977 kb on + strand, within GFF6167 at 72.978 kb on - strand, within GFF6167 at 72.978 kb on - strand, within GFF6167 at 73.058 kb on + strand, within GFF6167 at 73.059 kb on - strand, within GFF6167 at 73.059 kb on - strand, within GFF6167 at 73.088 kb on + strand, within GFF6167 at 73.145 kb on + strand, within GFF6167 at 73.145 kb on + strand, within GFF6167 at 73.322 kb on + strand, within GFF6167 at 73.323 kb on - strand, within GFF6167 at 73.361 kb on + strand at 73.362 kb on - strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.425 kb on - strand at 73.425 kb on - strand at 73.425 kb on - strand at 73.637 kb on - strand, within GFF6168 at 73.637 kb on - strand, within GFF6168 at 73.908 kb on + strand, within GFF6169 at 73.908 kb on + strand, within GFF6169 at 73.908 kb on + strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 74.294 kb on - strand, within GFF6170 at 74.378 kb on + strand, within GFF6170 at 74.378 kb on + strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 71,567 - -0.5 71,582 - +0.6 71,603 + -0.5 71,603 + -0.2 71,603 + +1.0 71,604 - +0.3 71,604 - +0.1 71,604 - +0.4 71,778 - GFF6166 0.19 +0.5 71,778 - GFF6166 0.19 +1.0 71,801 + GFF6166 0.22 +0.4 71,801 + GFF6166 0.22 -1.5 71,801 + GFF6166 0.22 -0.2 71,802 - GFF6166 0.23 +0.2 71,802 - GFF6166 0.23 -1.5 71,802 - GFF6166 0.23 -1.2 71,802 - GFF6166 0.23 -0.2 71,802 - GFF6166 0.23 -0.6 71,802 - GFF6166 0.23 -0.4 71,802 - GFF6166 0.23 -0.4 71,802 - GFF6166 0.23 -0.9 71,987 + GFF6166 0.51 +0.3 71,988 - GFF6166 0.51 -0.7 72,080 + GFF6166 0.65 -1.1 72,081 - GFF6166 0.65 -0.8 72,104 + GFF6166 0.68 -0.7 72,104 + GFF6166 0.68 -0.7 72,105 - GFF6166 0.68 +0.1 72,105 - GFF6166 0.68 -0.7 72,105 - GFF6166 0.68 +0.1 72,105 - GFF6166 0.68 -1.2 72,128 + GFF6166 0.72 +0.3 72,128 + GFF6166 0.72 +0.1 72,129 - GFF6166 0.72 +0.4 72,129 - GFF6166 0.72 +0.6 72,129 - GFF6166 0.72 -1.1 72,129 - GFF6166 0.72 +0.3 72,134 + GFF6166 0.73 -1.1 72,135 - GFF6166 0.73 -1.0 72,135 - GFF6166 0.73 +0.5 72,375 - -1.2 72,558 - GFF6167 0.14 -0.4 72,558 - GFF6167 0.14 -0.1 72,558 - GFF6167 0.14 +1.6 72,560 + GFF6167 0.14 -0.7 72,560 + GFF6167 0.14 -0.1 72,560 + GFF6167 0.14 +0.8 72,561 - GFF6167 0.14 +2.0 72,561 - GFF6167 0.14 +0.7 72,582 - GFF6167 0.16 -0.2 72,743 + GFF6167 0.32 +0.9 72,767 + GFF6167 0.34 -0.4 72,767 + GFF6167 0.34 -0.7 72,767 + GFF6167 0.34 -1.1 72,767 + GFF6167 0.34 +0.6 72,767 + GFF6167 0.34 +1.1 72,977 + GFF6167 0.55 -0.3 72,978 - GFF6167 0.55 -0.3 72,978 - GFF6167 0.55 -0.4 73,058 + GFF6167 0.63 -0.4 73,059 - GFF6167 0.63 +0.9 73,059 - GFF6167 0.63 +0.7 73,088 + GFF6167 0.66 +0.2 73,145 + GFF6167 0.72 +0.5 73,145 + GFF6167 0.72 +0.1 73,322 + GFF6167 0.90 +0.4 73,323 - GFF6167 0.90 -0.2 73,361 + +0.2 73,362 - +0.8 73,424 + +0.0 73,424 + -0.2 73,424 + -0.7 73,424 + -0.7 73,424 + -0.8 73,424 + -0.1 73,424 + -1.3 73,424 + +0.1 73,424 + +0.4 73,424 + -1.1 73,424 + +0.0 73,425 - -1.2 73,425 - -0.5 73,425 - -0.6 73,637 - GFF6168 0.51 -3.3 73,637 - GFF6168 0.51 +0.3 73,908 + GFF6169 0.33 +0.1 73,908 + GFF6169 0.33 +0.1 73,908 + GFF6169 0.33 +1.3 73,909 - GFF6169 0.33 +2.2 73,909 - GFF6169 0.33 +0.7 73,909 - GFF6169 0.33 -2.3 73,909 - GFF6169 0.33 -1.8 73,909 - GFF6169 0.33 +0.1 73,909 - GFF6169 0.33 +0.3 73,965 + GFF6169 0.53 +0.3 73,965 + GFF6169 0.53 -0.6 73,965 + GFF6169 0.53 -0.2 73,965 + GFF6169 0.53 -0.0 73,965 + GFF6169 0.53 -1.5 73,965 + GFF6169 0.53 -0.1 73,965 + GFF6169 0.53 +0.4 73,965 + GFF6169 0.53 -1.8 73,965 + GFF6169 0.53 +0.2 73,966 - GFF6169 0.53 +0.9 73,966 - GFF6169 0.53 -1.5 73,966 - GFF6169 0.53 +0.1 73,966 - GFF6169 0.53 +2.6 74,294 - GFF6170 0.41 -0.2 74,378 + GFF6170 0.58 +1.3 74,378 + GFF6170 0.58 -0.2 74,379 - GFF6170 0.58 -0.2 74,379 - GFF6170 0.58 -0.9 74,379 - GFF6170 0.58 +0.6 74,379 - GFF6170 0.58 +0.2 74,379 - GFF6170 0.58 -0.3
Or see this region's nucleotide sequence