Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6140 and GFF6141 are separated by 79 nucleotides GFF6141 and GFF6142 are separated by 41 nucleotides
GFF6140 - Uncharacterized MFS-type transporter Saci_1521, at 42,596 to 44,038
GFF6140
GFF6141 - 2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37), at 44,118 to 45,248
GFF6141
GFF6142 - 2-aminoethylphosphonate ABC transporter permease protein, at 45,290 to 47,023
GFF6142
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 43.131 kb on + strand, within GFF6140 at 43.132 kb on - strand, within GFF6140 at 43.156 kb on - strand, within GFF6140 at 43.156 kb on - strand, within GFF6140 at 43.176 kb on + strand, within GFF6140 at 43.176 kb on + strand, within GFF6140 at 43.176 kb on + strand, within GFF6140 at 43.293 kb on + strand, within GFF6140 at 43.294 kb on - strand, within GFF6140 at 43.741 kb on - strand, within GFF6140 at 43.833 kb on + strand, within GFF6140 at 43.833 kb on + strand, within GFF6140 at 43.833 kb on + strand, within GFF6140 at 44.030 kb on + strand at 44.049 kb on - strand at 44.076 kb on + strand at 44.076 kb on + strand at 44.267 kb on - strand, within GFF6141 at 44.275 kb on + strand, within GFF6141 at 44.275 kb on + strand, within GFF6141 at 44.276 kb on - strand, within GFF6141 at 44.276 kb on - strand, within GFF6141 at 44.276 kb on - strand, within GFF6141 at 44.299 kb on + strand, within GFF6141 at 44.300 kb on - strand, within GFF6141 at 44.317 kb on + strand, within GFF6141 at 44.317 kb on + strand, within GFF6141 at 44.437 kb on + strand, within GFF6141 at 44.476 kb on + strand, within GFF6141 at 44.476 kb on + strand, within GFF6141 at 44.477 kb on - strand, within GFF6141 at 44.477 kb on - strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.570 kb on - strand, within GFF6141 at 44.915 kb on - strand, within GFF6141 at 44.915 kb on - strand, within GFF6141 at 44.975 kb on - strand, within GFF6141 at 45.477 kb on + strand, within GFF6142 at 45.525 kb on + strand, within GFF6142 at 45.525 kb on + strand, within GFF6142 at 45.678 kb on + strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.681 kb on + strand, within GFF6142 at 45.681 kb on + strand, within GFF6142 at 45.682 kb on - strand, within GFF6142 at 45.682 kb on - strand, within GFF6142 at 45.871 kb on - strand, within GFF6142 at 46.006 kb on - strand, within GFF6142 at 46.032 kb on + strand, within GFF6142 at 46.129 kb on - strand, within GFF6142 at 46.179 kb on + strand, within GFF6142 at 46.180 kb on - strand, within GFF6142
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 43,131 + GFF6140 0.37 +0.1 43,132 - GFF6140 0.37 -0.4 43,156 - GFF6140 0.39 -0.4 43,156 - GFF6140 0.39 +0.5 43,176 + GFF6140 0.40 -0.2 43,176 + GFF6140 0.40 -0.6 43,176 + GFF6140 0.40 -1.8 43,293 + GFF6140 0.48 +0.4 43,294 - GFF6140 0.48 +1.2 43,741 - GFF6140 0.79 -1.3 43,833 + GFF6140 0.86 +0.3 43,833 + GFF6140 0.86 -0.3 43,833 + GFF6140 0.86 +0.5 44,030 + -1.2 44,049 - -2.0 44,076 + -0.3 44,076 + +0.1 44,267 - GFF6141 0.13 +0.1 44,275 + GFF6141 0.14 -0.2 44,275 + GFF6141 0.14 -2.4 44,276 - GFF6141 0.14 -0.2 44,276 - GFF6141 0.14 -0.2 44,276 - GFF6141 0.14 -0.5 44,299 + GFF6141 0.16 +0.2 44,300 - GFF6141 0.16 -0.2 44,317 + GFF6141 0.18 +0.4 44,317 + GFF6141 0.18 +0.2 44,437 + GFF6141 0.28 +0.1 44,476 + GFF6141 0.32 -0.5 44,476 + GFF6141 0.32 +0.8 44,477 - GFF6141 0.32 -1.4 44,477 - GFF6141 0.32 +0.3 44,554 + GFF6141 0.39 -0.6 44,554 + GFF6141 0.39 -0.1 44,554 + GFF6141 0.39 -0.5 44,554 + GFF6141 0.39 -0.4 44,555 - GFF6141 0.39 -1.4 44,555 - GFF6141 0.39 -0.1 44,555 - GFF6141 0.39 -1.1 44,555 - GFF6141 0.39 -0.5 44,570 - GFF6141 0.40 -1.5 44,915 - GFF6141 0.70 -0.7 44,915 - GFF6141 0.70 +0.2 44,975 - GFF6141 0.76 -0.7 45,477 + GFF6142 0.11 +0.3 45,525 + GFF6142 0.14 +0.4 45,525 + GFF6142 0.14 -0.1 45,678 + GFF6142 0.22 +0.1 45,679 - GFF6142 0.22 -0.4 45,679 - GFF6142 0.22 -0.4 45,679 - GFF6142 0.22 -0.3 45,681 + GFF6142 0.23 +0.6 45,681 + GFF6142 0.23 +0.8 45,682 - GFF6142 0.23 -0.1 45,682 - GFF6142 0.23 -0.5 45,871 - GFF6142 0.34 +0.1 46,006 - GFF6142 0.41 -0.1 46,032 + GFF6142 0.43 -1.2 46,129 - GFF6142 0.48 -1.2 46,179 + GFF6142 0.51 +1.1 46,180 - GFF6142 0.51 -1.2
Or see this region's nucleotide sequence