Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6132 and GFF6133 overlap by 4 nucleotides GFF6133 and GFF6134 are separated by 1 nucleotides GFF6134 and GFF6135 are separated by 138 nucleotides
GFF6132 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 33,442 to 34,245
GFF6132
GFF6133 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 34,242 to 35,105
GFF6133
GFF6134 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 35,107 to 35,988
GFF6134
GFF6135 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 36,127 to 37,281
GFF6135
Position (kb)
35
36 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 34.831 kb on + strand, within GFF6133 at 34.831 kb on + strand, within GFF6133 at 34.831 kb on + strand, within GFF6133 at 34.832 kb on - strand, within GFF6133 at 34.832 kb on - strand, within GFF6133 at 34.832 kb on - strand, within GFF6133 at 34.931 kb on - strand, within GFF6133 at 34.931 kb on - strand, within GFF6133 at 34.957 kb on + strand, within GFF6133 at 35.108 kb on + strand at 35.108 kb on + strand at 35.109 kb on - strand at 35.109 kb on - strand at 35.109 kb on - strand at 35.213 kb on + strand, within GFF6134 at 35.213 kb on + strand, within GFF6134 at 35.213 kb on + strand, within GFF6134 at 35.214 kb on - strand, within GFF6134 at 35.357 kb on + strand, within GFF6134 at 35.417 kb on + strand, within GFF6134 at 35.418 kb on - strand, within GFF6134 at 35.450 kb on + strand, within GFF6134 at 35.450 kb on + strand, within GFF6134 at 35.451 kb on - strand, within GFF6134 at 35.911 kb on + strand at 35.912 kb on - strand at 35.942 kb on + strand at 35.942 kb on + strand at 35.943 kb on - strand at 35.943 kb on - strand at 35.943 kb on - strand at 36.054 kb on + strand at 36.054 kb on + strand at 36.054 kb on + strand at 36.054 kb on + strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.055 kb on - strand at 36.120 kb on + strand at 36.128 kb on + strand at 36.128 kb on + strand at 36.128 kb on + strand at 36.128 kb on + strand at 36.129 kb on - strand at 36.129 kb on - strand at 36.129 kb on - strand at 36.129 kb on - strand at 36.129 kb on - strand at 36.191 kb on + strand at 36.350 kb on + strand, within GFF6135 at 36.350 kb on + strand, within GFF6135 at 36.350 kb on + strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.351 kb on - strand, within GFF6135 at 36.362 kb on + strand, within GFF6135 at 36.362 kb on + strand, within GFF6135 at 36.410 kb on + strand, within GFF6135 at 36.410 kb on + strand, within GFF6135 at 36.410 kb on + strand, within GFF6135 at 36.411 kb on - strand, within GFF6135 at 36.411 kb on - strand, within GFF6135 at 36.411 kb on - strand, within GFF6135 at 36.473 kb on + strand, within GFF6135 at 36.473 kb on + strand, within GFF6135 at 36.473 kb on + strand, within GFF6135 at 36.473 kb on + strand, within GFF6135 at 36.473 kb on + strand, within GFF6135 at 36.474 kb on - strand, within GFF6135 at 36.474 kb on - strand, within GFF6135 at 36.474 kb on - strand, within GFF6135 at 36.474 kb on - strand, within GFF6135 at 36.744 kb on - strand, within GFF6135 at 36.872 kb on + strand, within GFF6135 at 36.872 kb on + strand, within GFF6135 at 36.872 kb on + strand, within GFF6135 at 36.873 kb on - strand, within GFF6135 at 36.873 kb on - strand, within GFF6135 at 36.873 kb on - strand, within GFF6135
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 34,831 + GFF6133 0.68 -1.2 34,831 + GFF6133 0.68 -1.2 34,831 + GFF6133 0.68 -0.4 34,832 - GFF6133 0.68 +2.1 34,832 - GFF6133 0.68 -0.6 34,832 - GFF6133 0.68 +0.3 34,931 - GFF6133 0.80 -0.7 34,931 - GFF6133 0.80 +1.1 34,957 + GFF6133 0.83 -0.3 35,108 + -0.0 35,108 + -0.4 35,109 - -1.0 35,109 - +0.0 35,109 - -0.5 35,213 + GFF6134 0.12 -1.3 35,213 + GFF6134 0.12 +0.1 35,213 + GFF6134 0.12 -0.5 35,214 - GFF6134 0.12 -0.7 35,357 + GFF6134 0.28 +0.1 35,417 + GFF6134 0.35 -1.2 35,418 - GFF6134 0.35 +1.0 35,450 + GFF6134 0.39 -0.9 35,450 + GFF6134 0.39 -0.1 35,451 - GFF6134 0.39 -0.7 35,911 + -0.4 35,912 - -0.4 35,942 + -0.3 35,942 + +0.6 35,943 - -0.3 35,943 - +0.3 35,943 - -0.1 36,054 + -1.0 36,054 + +0.2 36,054 + +0.3 36,054 + -1.1 36,055 - -0.1 36,055 - -1.3 36,055 - -1.3 36,055 - -0.4 36,055 - +1.6 36,055 - +0.4 36,055 - -0.3 36,120 + +0.4 36,128 + -0.1 36,128 + +0.6 36,128 + -0.5 36,128 + -0.2 36,129 - +0.5 36,129 - +0.4 36,129 - +0.7 36,129 - +0.5 36,129 - -0.3 36,191 + +1.2 36,350 + GFF6135 0.19 -2.6 36,350 + GFF6135 0.19 -0.2 36,350 + GFF6135 0.19 +0.3 36,351 - GFF6135 0.19 -0.1 36,351 - GFF6135 0.19 -0.1 36,351 - GFF6135 0.19 -2.6 36,351 - GFF6135 0.19 -0.4 36,351 - GFF6135 0.19 -0.2 36,351 - GFF6135 0.19 -0.1 36,351 - GFF6135 0.19 +0.6 36,351 - GFF6135 0.19 +0.2 36,362 + GFF6135 0.20 -0.2 36,362 + GFF6135 0.20 -1.2 36,410 + GFF6135 0.25 -0.4 36,410 + GFF6135 0.25 +0.6 36,410 + GFF6135 0.25 -0.4 36,411 - GFF6135 0.25 -0.3 36,411 - GFF6135 0.25 +1.2 36,411 - GFF6135 0.25 +0.7 36,473 + GFF6135 0.30 -0.7 36,473 + GFF6135 0.30 -1.4 36,473 + GFF6135 0.30 +0.8 36,473 + GFF6135 0.30 +0.5 36,473 + GFF6135 0.30 +0.2 36,474 - GFF6135 0.30 -0.2 36,474 - GFF6135 0.30 +0.6 36,474 - GFF6135 0.30 -1.0 36,474 - GFF6135 0.30 -0.7 36,744 - GFF6135 0.53 -0.2 36,872 + GFF6135 0.65 -0.0 36,872 + GFF6135 0.65 +0.6 36,872 + GFF6135 0.65 -0.3 36,873 - GFF6135 0.65 -1.6 36,873 - GFF6135 0.65 -0.2 36,873 - GFF6135 0.65 -1.0
Or see this region's nucleotide sequence