Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6127 and GFF6128 are separated by 228 nucleotides GFF6128 and GFF6129 overlap by 4 nucleotides GFF6129 and GFF6130 are separated by 22 nucleotides
GFF6127 - Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit, at 28,139 to 29,281
GFF6127
GFF6128 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 29,510 to 29,776
GFF6128
GFF6129 - (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10), at 29,773 to 30,696
GFF6129
GFF6130 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 30,719 to 32,572
GFF6130
Position (kb) 29
30
31 Strain fitness (log2 ratio) -2
-1
0
1 at 28.869 kb on + strand, within GFF6127 at 28.870 kb on - strand, within GFF6127 at 28.953 kb on + strand, within GFF6127 at 28.953 kb on + strand, within GFF6127 at 28.954 kb on - strand, within GFF6127 at 28.954 kb on - strand, within GFF6127 at 29.163 kb on + strand, within GFF6127 at 29.164 kb on - strand, within GFF6127 at 29.164 kb on - strand, within GFF6127 at 29.205 kb on + strand at 29.205 kb on + strand at 29.205 kb on + strand at 29.205 kb on + strand at 29.205 kb on + strand at 29.206 kb on - strand at 29.206 kb on - strand at 29.270 kb on + strand at 29.270 kb on + strand at 29.271 kb on - strand at 29.271 kb on - strand at 29.400 kb on + strand at 29.401 kb on - strand at 29.478 kb on - strand at 29.478 kb on - strand at 29.561 kb on - strand, within GFF6128 at 29.582 kb on + strand, within GFF6128 at 29.666 kb on + strand, within GFF6128 at 29.666 kb on + strand, within GFF6128 at 29.667 kb on - strand, within GFF6128 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.550 kb on + strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.551 kb on - strand, within GFF6129 at 30.554 kb on + strand, within GFF6129 at 30.555 kb on - strand, within GFF6129 at 30.555 kb on - strand, within GFF6129
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 28,869 + GFF6127 0.64 -0.2 28,870 - GFF6127 0.64 +0.4 28,953 + GFF6127 0.71 +0.1 28,953 + GFF6127 0.71 +0.4 28,954 - GFF6127 0.71 +1.3 28,954 - GFF6127 0.71 -0.8 29,163 + GFF6127 0.90 -0.1 29,164 - GFF6127 0.90 +1.2 29,164 - GFF6127 0.90 -0.3 29,205 + -0.4 29,205 + +0.2 29,205 + -1.4 29,205 + +0.7 29,205 + -0.0 29,206 - +0.6 29,206 - +1.2 29,270 + -0.4 29,270 + +0.1 29,271 - -0.5 29,271 - +1.6 29,400 + -1.4 29,401 - +0.2 29,478 - -0.3 29,478 - -0.1 29,561 - GFF6128 0.19 -1.5 29,582 + GFF6128 0.27 -1.3 29,666 + GFF6128 0.58 -0.2 29,666 + GFF6128 0.58 -1.8 29,667 - GFF6128 0.59 -0.1 30,550 + GFF6129 0.84 -0.2 30,550 + GFF6129 0.84 -0.3 30,550 + GFF6129 0.84 +0.1 30,550 + GFF6129 0.84 +0.5 30,550 + GFF6129 0.84 -0.2 30,550 + GFF6129 0.84 -0.8 30,550 + GFF6129 0.84 -0.4 30,550 + GFF6129 0.84 +0.6 30,550 + GFF6129 0.84 -1.0 30,550 + GFF6129 0.84 -1.4 30,550 + GFF6129 0.84 -0.3 30,550 + GFF6129 0.84 -0.0 30,551 - GFF6129 0.84 -0.7 30,551 - GFF6129 0.84 -0.1 30,551 - GFF6129 0.84 +0.5 30,551 - GFF6129 0.84 -1.2 30,551 - GFF6129 0.84 -0.5 30,551 - GFF6129 0.84 +0.1 30,551 - GFF6129 0.84 -0.3 30,551 - GFF6129 0.84 -0.6 30,551 - GFF6129 0.84 +0.4 30,551 - GFF6129 0.84 -0.2 30,551 - GFF6129 0.84 +0.9 30,554 + GFF6129 0.85 -0.9 30,555 - GFF6129 0.85 +1.4 30,555 - GFF6129 0.85 +0.0
Or see this region's nucleotide sequence