Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6010 and GFF6011 are separated by 6 nucleotides GFF6011 and GFF6012 are separated by 111 nucleotides GFF6012 and GFF6013 are separated by 90 nucleotides
GFF6010 - MmgE/PrpD family protein, at 69,791 to 71,152
GFF6010
GFF6011 - putative decarboxylase, at 71,159 to 71,866
GFF6011
GFF6012 - Tricarboxylate transport protein TctC, at 71,978 to 72,982
GFF6012
GFF6013 - Muconate cycloisomerase (EC 5.5.1.1), at 73,073 to 74,227
GFF6013
Position (kb) 71
72
73 Strain fitness (log2 ratio) -1
0
1 at 71.057 kb on + strand at 71.057 kb on + strand at 71.058 kb on - strand at 71.122 kb on + strand at 71.463 kb on + strand, within GFF6011 at 71.497 kb on - strand, within GFF6011 at 71.522 kb on + strand, within GFF6011 at 71.523 kb on - strand, within GFF6011 at 71.731 kb on - strand, within GFF6011 at 71.873 kb on - strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.999 kb on - strand at 72.075 kb on + strand at 72.178 kb on + strand, within GFF6012 at 72.489 kb on + strand, within GFF6012 at 72.873 kb on + strand, within GFF6012 at 73.237 kb on - strand, within GFF6013 at 73.243 kb on - strand, within GFF6013 at 73.620 kb on + strand, within GFF6013 at 73.620 kb on + strand, within GFF6013 at 73.620 kb on + strand, within GFF6013 at 73.621 kb on - strand, within GFF6013 at 73.621 kb on - strand, within GFF6013 at 73.834 kb on - strand, within GFF6013 at 73.947 kb on + strand, within GFF6013 at 73.980 kb on + strand, within GFF6013
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 71,057 + -1.2 71,057 + -0.3 71,058 - -1.1 71,122 + +0.7 71,463 + GFF6011 0.43 +0.0 71,497 - GFF6011 0.48 -0.0 71,522 + GFF6011 0.51 +0.2 71,523 - GFF6011 0.51 -1.5 71,731 - GFF6011 0.81 +0.3 71,873 - -1.1 71,998 + +1.1 71,998 + -0.2 71,998 + -0.8 71,999 - -0.3 72,075 + -0.2 72,178 + GFF6012 0.20 -0.1 72,489 + GFF6012 0.51 +0.2 72,873 + GFF6012 0.89 -0.5 73,237 - GFF6013 0.14 +0.2 73,243 - GFF6013 0.15 -0.1 73,620 + GFF6013 0.47 -0.1 73,620 + GFF6013 0.47 -0.8 73,620 + GFF6013 0.47 -0.8 73,621 - GFF6013 0.47 +0.8 73,621 - GFF6013 0.47 -0.2 73,834 - GFF6013 0.66 +0.8 73,947 + GFF6013 0.76 +0.7 73,980 + GFF6013 0.79 -0.6
Or see this region's nucleotide sequence