Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6003 and GFF6004 are separated by 66 nucleotides GFF6004 and GFF6005 are separated by 112 nucleotides GFF6005 and GFF6006 are separated by 3 nucleotides
GFF6003 - no description, at 61,874 to 62,488
GFF6003
GFF6004 - hypothetical protein, at 62,555 to 62,734
GFF6004
GFF6005 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 62,847 to 64,352
GFF6005
GFF6006 - BUG/TctC family periplasmic protein, at 64,356 to 65,342
GFF6006
Position (kb) 62
63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 62.019 kb on + strand, within GFF6003 at 62.020 kb on - strand, within GFF6003 at 62.020 kb on - strand, within GFF6003 at 62.479 kb on + strand at 62.479 kb on + strand at 62.508 kb on + strand at 62.508 kb on + strand at 62.508 kb on + strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.535 kb on + strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.548 kb on - strand at 62.571 kb on + strand at 62.571 kb on + strand at 62.572 kb on - strand at 62.572 kb on - strand at 62.658 kb on + strand, within GFF6004 at 62.848 kb on + strand at 62.948 kb on - strand at 63.272 kb on - strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.440 kb on - strand, within GFF6005 at 63.440 kb on - strand, within GFF6005 at 63.455 kb on - strand, within GFF6005 at 63.532 kb on + strand, within GFF6005 at 63.532 kb on + strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.538 kb on + strand, within GFF6005 at 63.539 kb on - strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.766 kb on + strand, within GFF6005 at 63.766 kb on + strand, within GFF6005 at 64.255 kb on + strand at 64.255 kb on + strand at 64.255 kb on + strand at 64.255 kb on + strand at 64.256 kb on - strand at 64.357 kb on + strand at 64.453 kb on + strand at 64.454 kb on - strand at 64.586 kb on - strand, within GFF6006 at 64.859 kb on - strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.952 kb on - strand, within GFF6006
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 62,019 + GFF6003 0.24 +0.1 62,020 - GFF6003 0.24 +0.3 62,020 - GFF6003 0.24 -0.5 62,479 + +0.6 62,479 + +0.8 62,508 + -0.6 62,508 + +1.2 62,508 + -1.8 62,509 - +0.7 62,509 - -0.5 62,509 - -0.1 62,509 - -1.0 62,509 - +1.3 62,535 + -0.4 62,536 - -0.3 62,536 - -0.8 62,548 - -0.3 62,571 + +1.2 62,571 + -0.8 62,572 - +0.1 62,572 - +0.1 62,658 + GFF6004 0.57 -0.6 62,848 + -1.5 62,948 - -0.3 63,272 - GFF6005 0.28 +0.7 63,303 + GFF6005 0.30 +0.0 63,303 + GFF6005 0.30 +0.6 63,303 + GFF6005 0.30 +0.8 63,304 - GFF6005 0.30 -0.7 63,304 - GFF6005 0.30 -0.1 63,304 - GFF6005 0.30 -1.0 63,304 - GFF6005 0.30 -0.1 63,304 - GFF6005 0.30 -1.5 63,304 - GFF6005 0.30 +0.1 63,440 - GFF6005 0.39 -0.4 63,440 - GFF6005 0.39 -0.5 63,455 - GFF6005 0.40 +0.6 63,532 + GFF6005 0.45 +0.3 63,532 + GFF6005 0.45 -1.1 63,533 - GFF6005 0.46 +0.0 63,533 - GFF6005 0.46 -0.5 63,533 - GFF6005 0.46 +0.3 63,538 + GFF6005 0.46 -0.5 63,539 - GFF6005 0.46 +0.1 63,601 + GFF6005 0.50 +0.2 63,601 + GFF6005 0.50 -0.1 63,601 + GFF6005 0.50 -0.4 63,601 + GFF6005 0.50 -1.8 63,601 + GFF6005 0.50 -0.6 63,601 + GFF6005 0.50 +0.3 63,601 + GFF6005 0.50 -0.5 63,601 + GFF6005 0.50 -0.3 63,602 - GFF6005 0.50 +0.9 63,602 - GFF6005 0.50 +0.1 63,602 - GFF6005 0.50 +0.7 63,602 - GFF6005 0.50 +0.1 63,602 - GFF6005 0.50 +0.6 63,602 - GFF6005 0.50 -0.2 63,766 + GFF6005 0.61 +0.1 63,766 + GFF6005 0.61 +0.1 64,255 + +0.1 64,255 + +1.2 64,255 + +0.8 64,255 + -0.3 64,256 - +0.4 64,357 + -0.8 64,453 + +0.1 64,454 - +0.4 64,586 - GFF6006 0.23 -0.6 64,859 - GFF6006 0.51 +0.5 64,951 + GFF6006 0.60 -0.0 64,951 + GFF6006 0.60 +0.1 64,951 + GFF6006 0.60 -0.1 64,952 - GFF6006 0.60 +1.1
Or see this region's nucleotide sequence