Strain Fitness in Variovorax sp. SCN45 around GFF5721

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5719 and GFF5720 are separated by 76 nucleotidesGFF5720 and GFF5721 overlap by 1 nucleotidesGFF5721 and GFF5722 are separated by 2 nucleotides GFF5719 - Flagellar basal-body rod protein FlgF, at 88,808 to 89,554 GFF5719 GFF5720 - Flagellar basal-body rod protein FlgG, at 89,631 to 90,413 GFF5720 GFF5721 - Flagellar L-ring protein FlgH, at 90,413 to 91,120 GFF5721 GFF5722 - Flagellar P-ring protein FlgI, at 91,123 to 92,331 GFF5722 Position (kb) 90 91 92Strain fitness (log2 ratio) -2 -1 0 1at 89.546 kb on + strandat 89.646 kb on + strandat 89.646 kb on + strandat 89.767 kb on - strand, within GFF5720at 89.970 kb on + strand, within GFF5720at 90.361 kb on - strandat 90.951 kb on - strand, within GFF5721at 90.951 kb on - strand, within GFF5721at 90.951 kb on - strand, within GFF5721at 91.041 kb on - strand, within GFF5721at 91.055 kb on + strandat 91.640 kb on - strand, within GFF5722at 91.640 kb on - strand, within GFF5722

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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89,546 + +0.8
89,646 + -0.4
89,646 + +1.5
89,767 - GFF5720 0.17 -1.4
89,970 + GFF5720 0.43 -0.4
90,361 - -1.0
90,951 - GFF5721 0.76 -0.5
90,951 - GFF5721 0.76 -0.5
90,951 - GFF5721 0.76 -2.0
91,041 - GFF5721 0.89 -0.4
91,055 + +0.2
91,640 - GFF5722 0.43 -0.1
91,640 - GFF5722 0.43 +0.8

Or see this region's nucleotide sequence