Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5598 and GFF5599 overlap by 4 nucleotides GFF5599 and GFF5600 overlap by 4 nucleotides GFF5600 and GFF5601 are separated by 9 nucleotides GFF5601 and GFF5602 are separated by 132 nucleotides GFF5602 and GFF5603 are separated by 186 nucleotides
GFF5598 - putative phospholipase D family protein, at 218,379 to 219,659
GFF5598
GFF5599 - hypothetical protein, at 219,656 to 220,087
GFF5599
GFF5600 - no description, at 220,084 to 220,224
GFF5600
GFF5601 - Predicted hydrolase of the alpha/beta-hydrolase fold, at 220,234 to 220,920
GFF5601
GFF5602 - FIG00348944: hypothetical protein, at 221,053 to 221,559
GFF5602
GFF5603 - D-xylose ABC transporter, substrate-binding protein XylF, at 221,746 to 222,768
GFF5603
Position (kb) 220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 219.390 kb on + strand, within GFF5598 at 219.510 kb on + strand, within GFF5598 at 219.510 kb on + strand, within GFF5598 at 219.510 kb on + strand, within GFF5598 at 219.511 kb on - strand, within GFF5598 at 219.511 kb on - strand, within GFF5598 at 219.582 kb on + strand at 219.582 kb on + strand at 219.582 kb on + strand at 219.582 kb on + strand at 219.583 kb on - strand at 219.583 kb on - strand at 219.583 kb on - strand at 219.583 kb on - strand at 219.657 kb on + strand at 219.657 kb on + strand at 219.657 kb on + strand at 219.658 kb on - strand at 219.658 kb on - strand at 219.658 kb on - strand at 219.658 kb on - strand at 219.658 kb on - strand at 219.750 kb on + strand, within GFF5599 at 219.795 kb on + strand, within GFF5599 at 219.795 kb on + strand, within GFF5599 at 219.795 kb on + strand, within GFF5599 at 219.795 kb on + strand, within GFF5599 at 219.796 kb on - strand, within GFF5599 at 219.796 kb on - strand, within GFF5599 at 219.796 kb on - strand, within GFF5599 at 219.796 kb on - strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.813 kb on + strand, within GFF5599 at 219.814 kb on - strand, within GFF5599 at 219.814 kb on - strand, within GFF5599 at 219.814 kb on - strand, within GFF5599 at 219.814 kb on - strand, within GFF5599 at 219.814 kb on - strand, within GFF5599 at 219.858 kb on + strand, within GFF5599 at 219.858 kb on + strand, within GFF5599 at 219.858 kb on + strand, within GFF5599 at 220.159 kb on + strand, within GFF5600 at 220.159 kb on + strand, within GFF5600 at 220.160 kb on - strand, within GFF5600 at 220.160 kb on - strand, within GFF5600 at 220.178 kb on - strand, within GFF5600 at 220.239 kb on + strand at 220.239 kb on + strand at 220.239 kb on + strand at 220.239 kb on + strand at 220.239 kb on + strand at 220.239 kb on + strand at 220.240 kb on - strand at 220.240 kb on - strand at 220.240 kb on - strand at 220.432 kb on + strand, within GFF5601 at 220.432 kb on + strand, within GFF5601 at 220.433 kb on - strand, within GFF5601 at 220.433 kb on - strand, within GFF5601 at 220.433 kb on - strand, within GFF5601 at 221.059 kb on + strand at 221.059 kb on + strand at 221.059 kb on + strand at 221.059 kb on + strand at 221.059 kb on + strand at 221.059 kb on + strand at 221.059 kb on + strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.060 kb on - strand at 221.170 kb on + strand, within GFF5602 at 221.171 kb on - strand, within GFF5602 at 221.222 kb on + strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.223 kb on - strand, within GFF5602 at 221.288 kb on - strand, within GFF5602 at 221.431 kb on + strand, within GFF5602 at 221.671 kb on + strand at 221.689 kb on - strand at 221.692 kb on + strand at 221.700 kb on + strand at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.908 kb on + strand, within GFF5603 at 221.909 kb on - strand, within GFF5603 at 221.909 kb on - strand, within GFF5603 at 221.909 kb on - strand, within GFF5603 at 221.909 kb on - strand, within GFF5603
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 219,390 + GFF5598 0.79 +0.2 219,510 + GFF5598 0.88 -0.5 219,510 + GFF5598 0.88 +2.4 219,510 + GFF5598 0.88 -0.5 219,511 - GFF5598 0.88 +0.8 219,511 - GFF5598 0.88 -0.8 219,582 + +0.3 219,582 + -0.6 219,582 + +1.7 219,582 + -0.1 219,583 - +0.0 219,583 - +0.1 219,583 - -0.3 219,583 - +0.4 219,657 + -1.3 219,657 + +0.2 219,657 + +0.2 219,658 - +0.7 219,658 - -0.4 219,658 - -1.0 219,658 - +0.9 219,658 - +0.1 219,750 + GFF5599 0.22 +0.4 219,795 + GFF5599 0.32 -0.6 219,795 + GFF5599 0.32 -0.0 219,795 + GFF5599 0.32 -0.7 219,795 + GFF5599 0.32 +0.3 219,796 - GFF5599 0.32 +0.4 219,796 - GFF5599 0.32 +0.7 219,796 - GFF5599 0.32 +0.6 219,796 - GFF5599 0.32 +1.6 219,813 + GFF5599 0.36 +0.5 219,813 + GFF5599 0.36 -0.5 219,813 + GFF5599 0.36 -0.6 219,813 + GFF5599 0.36 -0.1 219,813 + GFF5599 0.36 +0.5 219,813 + GFF5599 0.36 +0.3 219,814 - GFF5599 0.37 -0.2 219,814 - GFF5599 0.37 -1.3 219,814 - GFF5599 0.37 +0.2 219,814 - GFF5599 0.37 -0.4 219,814 - GFF5599 0.37 -1.3 219,858 + GFF5599 0.47 -0.1 219,858 + GFF5599 0.47 -0.7 219,858 + GFF5599 0.47 +0.0 220,159 + GFF5600 0.53 -0.9 220,159 + GFF5600 0.53 -0.0 220,160 - GFF5600 0.54 +1.4 220,160 - GFF5600 0.54 -0.2 220,178 - GFF5600 0.67 -0.3 220,239 + -0.7 220,239 + -0.8 220,239 + -0.3 220,239 + +0.6 220,239 + -0.3 220,239 + -1.5 220,240 - -0.2 220,240 - -0.0 220,240 - -0.4 220,432 + GFF5601 0.29 -0.2 220,432 + GFF5601 0.29 -2.2 220,433 - GFF5601 0.29 -1.1 220,433 - GFF5601 0.29 -0.8 220,433 - GFF5601 0.29 -0.9 221,059 + -1.6 221,059 + -0.6 221,059 + -1.0 221,059 + +0.2 221,059 + +1.6 221,059 + +0.8 221,059 + -1.7 221,060 - -1.3 221,060 - -0.6 221,060 - -1.3 221,060 - -1.2 221,060 - +1.0 221,060 - -0.4 221,060 - +0.2 221,170 + GFF5602 0.23 -0.2 221,171 - GFF5602 0.23 +1.0 221,222 + GFF5602 0.33 -0.6 221,223 - GFF5602 0.34 +0.4 221,223 - GFF5602 0.34 -0.2 221,223 - GFF5602 0.34 -0.7 221,223 - GFF5602 0.34 +1.0 221,223 - GFF5602 0.34 -0.3 221,223 - GFF5602 0.34 +1.2 221,288 - GFF5602 0.46 -1.2 221,431 + GFF5602 0.75 -0.7 221,671 + -0.6 221,689 - -0.3 221,692 + -1.3 221,700 + -1.5 221,908 + GFF5603 0.16 -1.4 221,908 + GFF5603 0.16 -2.2 221,908 + GFF5603 0.16 -2.3 221,908 + GFF5603 0.16 -1.7 221,908 + GFF5603 0.16 -1.4 221,908 + GFF5603 0.16 -2.9 221,908 + GFF5603 0.16 +2.0 221,909 - GFF5603 0.16 -1.4 221,909 - GFF5603 0.16 -1.5 221,909 - GFF5603 0.16 -1.7 221,909 - GFF5603 0.16 -2.3
Or see this region's nucleotide sequence