Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5575 and GFF5576 are separated by 5 nucleotides GFF5576 and GFF5577 are separated by 4 nucleotides GFF5577 and GFF5578 overlap by 4 nucleotides
GFF5575 - Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, at 194,802 to 196,424
GFF5575
GFF5576 - Acyl-CoA dehydrogenase, at 196,430 to 197,623
GFF5576
GFF5577 - Enoyl-CoA hydratase (EC 4.2.1.17), at 197,628 to 198,482
GFF5577
GFF5578 - Transcriptional regulator, MarR family, at 198,479 to 198,958
GFF5578
Position (kb) 196
197
198 Strain fitness (log2 ratio) -1
0
1
2
3 at 195.541 kb on + strand, within GFF5575 at 195.542 kb on - strand, within GFF5575 at 195.542 kb on - strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.560 kb on - strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.632 kb on - strand, within GFF5575 at 195.632 kb on - strand, within GFF5575 at 196.231 kb on + strand, within GFF5575 at 196.231 kb on + strand, within GFF5575 at 196.232 kb on - strand, within GFF5575 at 196.427 kb on + strand at 196.427 kb on + strand at 196.427 kb on + strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.431 kb on + strand at 196.432 kb on - strand at 196.432 kb on - strand at 196.488 kb on + strand at 196.488 kb on + strand at 196.488 kb on + strand at 196.489 kb on - strand at 196.516 kb on - strand at 196.698 kb on + strand, within GFF5576 at 196.698 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 197.094 kb on + strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.299 kb on + strand, within GFF5576 at 197.355 kb on + strand, within GFF5576 at 197.356 kb on - strand, within GFF5576 at 197.437 kb on - strand, within GFF5576 at 197.674 kb on + strand at 198.046 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.290 kb on - strand, within GFF5577 at 198.394 kb on + strand, within GFF5577 at 198.395 kb on - strand, within GFF5577 at 198.421 kb on + strand at 198.422 kb on - strand at 198.422 kb on - strand at 198.422 kb on - strand at 198.472 kb on + strand at 198.472 kb on + strand at 198.473 kb on - strand at 198.473 kb on - strand at 198.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 195,541 + GFF5575 0.46 -1.4 195,542 - GFF5575 0.46 -1.1 195,542 - GFF5575 0.46 -0.3 195,559 + GFF5575 0.47 +0.6 195,559 + GFF5575 0.47 +0.6 195,559 + GFF5575 0.47 -0.1 195,560 - GFF5575 0.47 +1.8 195,631 + GFF5575 0.51 +1.2 195,631 + GFF5575 0.51 +0.2 195,631 + GFF5575 0.51 -1.4 195,632 - GFF5575 0.51 +0.5 195,632 - GFF5575 0.51 -0.4 196,231 + GFF5575 0.88 -0.8 196,231 + GFF5575 0.88 -0.8 196,232 - GFF5575 0.88 -0.1 196,427 + +0.3 196,427 + +0.3 196,427 + +0.0 196,428 - -0.6 196,428 - +2.0 196,428 - -0.6 196,428 - +0.1 196,431 + -0.3 196,432 - -0.2 196,432 - -0.2 196,488 + -0.3 196,488 + -0.7 196,488 + -0.8 196,489 - -0.5 196,516 - -0.5 196,698 + GFF5576 0.22 -0.5 196,698 + GFF5576 0.22 -1.1 196,702 + GFF5576 0.23 -0.8 196,702 + GFF5576 0.23 +0.5 196,702 + GFF5576 0.23 -0.4 196,702 + GFF5576 0.23 +0.8 196,703 - GFF5576 0.23 -0.8 196,703 - GFF5576 0.23 +0.0 196,703 - GFF5576 0.23 +0.6 196,703 - GFF5576 0.23 -0.1 196,703 - GFF5576 0.23 -1.1 196,703 - GFF5576 0.23 -1.1 197,094 + GFF5576 0.56 +1.4 197,095 - GFF5576 0.56 -0.3 197,095 - GFF5576 0.56 +0.4 197,095 - GFF5576 0.56 +0.7 197,095 - GFF5576 0.56 -0.2 197,263 - GFF5576 0.70 +0.0 197,263 - GFF5576 0.70 -1.1 197,299 + GFF5576 0.73 +0.4 197,355 + GFF5576 0.77 +1.0 197,356 - GFF5576 0.78 +0.8 197,437 - GFF5576 0.84 +3.0 197,674 + +0.4 198,046 + GFF5577 0.49 -0.0 198,289 + GFF5577 0.77 -1.1 198,289 + GFF5577 0.77 -0.3 198,289 + GFF5577 0.77 +0.5 198,290 - GFF5577 0.77 -1.5 198,394 + GFF5577 0.90 +2.0 198,395 - GFF5577 0.90 -1.1 198,421 + -0.4 198,422 - -1.2 198,422 - +0.1 198,422 - -0.3 198,472 + -0.3 198,472 + -0.1 198,473 - -0.1 198,473 - -0.4 198,473 - -1.2
Or see this region's nucleotide sequence