Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5535 and GFF5536 are separated by 145 nucleotides GFF5536 and GFF5537 overlap by 4 nucleotides
GFF5535 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17), at 163,695 to 164,738
GFF5535
GFF5536 - no description, at 164,884 to 165,558
GFF5536
GFF5537 - Cell division inhibitor SulA, at 165,555 to 167,051
GFF5537
Position (kb)
164
165
166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 164.018 kb on + strand, within GFF5535 at 164.035 kb on + strand, within GFF5535 at 164.036 kb on - strand, within GFF5535 at 164.269 kb on + strand, within GFF5535 at 164.269 kb on + strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.270 kb on - strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.311 kb on + strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.312 kb on - strand, within GFF5535 at 164.450 kb on - strand, within GFF5535 at 164.852 kb on - strand at 165.037 kb on + strand, within GFF5536 at 165.037 kb on + strand, within GFF5536 at 165.037 kb on + strand, within GFF5536 at 165.037 kb on + strand, within GFF5536 at 165.037 kb on + strand, within GFF5536 at 165.037 kb on + strand, within GFF5536 at 165.038 kb on - strand, within GFF5536 at 165.038 kb on - strand, within GFF5536 at 165.130 kb on + strand, within GFF5536 at 165.131 kb on - strand, within GFF5536 at 165.131 kb on - strand, within GFF5536 at 165.166 kb on + strand, within GFF5536 at 165.166 kb on + strand, within GFF5536 at 165.166 kb on + strand, within GFF5536 at 165.166 kb on + strand, within GFF5536 at 165.167 kb on - strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.193 kb on + strand, within GFF5536 at 165.194 kb on - strand, within GFF5536 at 165.194 kb on - strand, within GFF5536 at 165.194 kb on - strand, within GFF5536 at 165.208 kb on + strand, within GFF5536 at 165.208 kb on + strand, within GFF5536 at 165.283 kb on + strand, within GFF5536 at 165.283 kb on + strand, within GFF5536 at 165.283 kb on + strand, within GFF5536 at 165.284 kb on - strand, within GFF5536 at 165.284 kb on - strand, within GFF5536 at 165.284 kb on - strand, within GFF5536 at 165.284 kb on - strand, within GFF5536 at 165.284 kb on - strand, within GFF5536 at 165.361 kb on + strand, within GFF5536 at 165.362 kb on - strand, within GFF5536 at 165.362 kb on - strand, within GFF5536 at 165.495 kb on + strand at 165.630 kb on + strand at 165.630 kb on + strand at 165.630 kb on + strand at 165.630 kb on + strand at 165.630 kb on + strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.631 kb on - strand at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.714 kb on + strand, within GFF5537 at 165.715 kb on - strand, within GFF5537 at 165.724 kb on + strand, within GFF5537 at 166.470 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.473 kb on + strand, within GFF5537 at 166.474 kb on - strand, within GFF5537 at 166.474 kb on - strand, within GFF5537
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 164,018 + GFF5535 0.31 +0.7 164,035 + GFF5535 0.33 +0.1 164,036 - GFF5535 0.33 -1.2 164,269 + GFF5535 0.55 +0.8 164,269 + GFF5535 0.55 +0.2 164,270 - GFF5535 0.55 +0.8 164,270 - GFF5535 0.55 -0.6 164,270 - GFF5535 0.55 -1.0 164,270 - GFF5535 0.55 +0.5 164,270 - GFF5535 0.55 +0.6 164,270 - GFF5535 0.55 -0.6 164,270 - GFF5535 0.55 +0.8 164,311 + GFF5535 0.59 -2.5 164,311 + GFF5535 0.59 -1.1 164,311 + GFF5535 0.59 +1.0 164,311 + GFF5535 0.59 -0.0 164,311 + GFF5535 0.59 +0.2 164,311 + GFF5535 0.59 +1.1 164,311 + GFF5535 0.59 +1.6 164,311 + GFF5535 0.59 +2.0 164,311 + GFF5535 0.59 +0.1 164,311 + GFF5535 0.59 -1.2 164,311 + GFF5535 0.59 +0.4 164,312 - GFF5535 0.59 -0.3 164,312 - GFF5535 0.59 -0.1 164,312 - GFF5535 0.59 +0.4 164,312 - GFF5535 0.59 +0.5 164,312 - GFF5535 0.59 -0.1 164,312 - GFF5535 0.59 +0.0 164,312 - GFF5535 0.59 +0.1 164,312 - GFF5535 0.59 -0.4 164,312 - GFF5535 0.59 -0.1 164,312 - GFF5535 0.59 +1.2 164,312 - GFF5535 0.59 -0.3 164,312 - GFF5535 0.59 -0.0 164,312 - GFF5535 0.59 -0.2 164,450 - GFF5535 0.72 +0.3 164,852 - +0.7 165,037 + GFF5536 0.23 +0.4 165,037 + GFF5536 0.23 -0.4 165,037 + GFF5536 0.23 +0.1 165,037 + GFF5536 0.23 +1.6 165,037 + GFF5536 0.23 +0.7 165,037 + GFF5536 0.23 -0.1 165,038 - GFF5536 0.23 -0.5 165,038 - GFF5536 0.23 +0.2 165,130 + GFF5536 0.36 -1.7 165,131 - GFF5536 0.37 +0.0 165,131 - GFF5536 0.37 +0.8 165,166 + GFF5536 0.42 -0.1 165,166 + GFF5536 0.42 -1.0 165,166 + GFF5536 0.42 -0.1 165,166 + GFF5536 0.42 -0.1 165,167 - GFF5536 0.42 -0.4 165,193 + GFF5536 0.46 -0.3 165,193 + GFF5536 0.46 -0.8 165,193 + GFF5536 0.46 -0.5 165,193 + GFF5536 0.46 -0.2 165,193 + GFF5536 0.46 -0.3 165,193 + GFF5536 0.46 -0.2 165,193 + GFF5536 0.46 -0.2 165,193 + GFF5536 0.46 +0.8 165,193 + GFF5536 0.46 +0.2 165,193 + GFF5536 0.46 -0.4 165,193 + GFF5536 0.46 +2.0 165,193 + GFF5536 0.46 +0.1 165,193 + GFF5536 0.46 -0.2 165,194 - GFF5536 0.46 -0.2 165,194 - GFF5536 0.46 +0.3 165,194 - GFF5536 0.46 +0.6 165,208 + GFF5536 0.48 -2.3 165,208 + GFF5536 0.48 -1.6 165,283 + GFF5536 0.59 +1.3 165,283 + GFF5536 0.59 -1.5 165,283 + GFF5536 0.59 -0.5 165,284 - GFF5536 0.59 +0.8 165,284 - GFF5536 0.59 +0.1 165,284 - GFF5536 0.59 +0.7 165,284 - GFF5536 0.59 +0.4 165,284 - GFF5536 0.59 -0.0 165,361 + GFF5536 0.71 +0.4 165,362 - GFF5536 0.71 -1.1 165,362 - GFF5536 0.71 -0.3 165,495 + -0.7 165,630 + -0.7 165,630 + -0.4 165,630 + +0.7 165,630 + +0.1 165,630 + +0.6 165,631 - -1.2 165,631 - +0.4 165,631 - -0.2 165,631 - -0.4 165,631 - +0.3 165,631 - +1.5 165,631 - +0.7 165,714 + GFF5537 0.11 -2.0 165,714 + GFF5537 0.11 -0.8 165,714 + GFF5537 0.11 -0.0 165,714 + GFF5537 0.11 +0.4 165,714 + GFF5537 0.11 -0.1 165,714 + GFF5537 0.11 -0.0 165,714 + GFF5537 0.11 +0.2 165,715 - GFF5537 0.11 -0.7 165,724 + GFF5537 0.11 -0.2 166,470 + GFF5537 0.61 -0.7 166,473 + GFF5537 0.61 +0.1 166,473 + GFF5537 0.61 -0.7 166,473 + GFF5537 0.61 -0.0 166,473 + GFF5537 0.61 -0.4 166,473 + GFF5537 0.61 -1.2 166,473 + GFF5537 0.61 +0.3 166,473 + GFF5537 0.61 +2.0 166,473 + GFF5537 0.61 -0.8 166,473 + GFF5537 0.61 +1.0 166,474 - GFF5537 0.61 +0.3 166,474 - GFF5537 0.61 +1.3
Or see this region's nucleotide sequence