Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5277 and GFF5278 are separated by 22 nucleotides GFF5278 and GFF5279 are separated by 30 nucleotides GFF5279 and GFF5280 are separated by 11 nucleotides GFF5280 and GFF5281 are separated by 26 nucleotides
GFF5277 - Sorbitol dehydrogenase (EC 1.1.1.14), at 145,866 to 146,654
GFF5277
GFF5278 - Transaldolase (EC 2.2.1.2), at 146,677 to 147,348
GFF5278
GFF5279 - Fructokinase (EC 2.7.1.4), at 147,379 to 148,347
GFF5279
GFF5280 - Transcriptional regulator of various polyols utilization, AraC family, at 148,359 to 149,315
GFF5280
GFF5281 - Xylulose kinase (EC 2.7.1.17), at 149,342 to 150,808
GFF5281
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 146.407 kb on + strand, within GFF5277 at 146.407 kb on + strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.604 kb on + strand at 146.605 kb on - strand at 146.698 kb on + strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 147.031 kb on + strand, within GFF5278 at 147.032 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.088 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.661 kb on + strand, within GFF5279 at 147.662 kb on - strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.880 kb on + strand, within GFF5279 at 148.074 kb on + strand, within GFF5279 at 148.074 kb on + strand, within GFF5279 at 148.075 kb on - strand, within GFF5279 at 148.075 kb on - strand, within GFF5279 at 148.323 kb on + strand at 148.324 kb on - strand at 148.324 kb on - strand at 148.447 kb on - strand at 148.533 kb on + strand, within GFF5280 at 148.533 kb on + strand, within GFF5280 at 148.533 kb on + strand, within GFF5280 at 148.533 kb on + strand, within GFF5280 at 148.534 kb on - strand, within GFF5280 at 148.534 kb on - strand, within GFF5280 at 148.534 kb on - strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.175 kb on + strand, within GFF5280 at 149.176 kb on - strand, within GFF5280 at 149.176 kb on - strand, within GFF5280 at 149.176 kb on - strand, within GFF5280 at 149.176 kb on - strand, within GFF5280 at 149.193 kb on + strand, within GFF5280 at 149.313 kb on + strand at 149.313 kb on + strand at 149.313 kb on + strand at 149.313 kb on + strand at 149.313 kb on + strand at 149.313 kb on + strand at 149.314 kb on - strand at 149.314 kb on - strand at 149.314 kb on - strand at 149.317 kb on + strand at 149.317 kb on + strand at 149.317 kb on + strand at 149.317 kb on + strand at 149.317 kb on + strand at 149.317 kb on + strand at 149.318 kb on - strand at 149.318 kb on - strand at 149.318 kb on - strand at 149.318 kb on - strand at 149.318 kb on - strand at 149.318 kb on - strand at 149.345 kb on + strand at 149.345 kb on + strand at 149.346 kb on - strand at 149.346 kb on - strand at 149.346 kb on - strand at 149.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 146,407 + GFF5277 0.69 +0.0 146,407 + GFF5277 0.69 -1.2 146,408 - GFF5277 0.69 -1.2 146,408 - GFF5277 0.69 -2.0 146,604 + +0.5 146,605 - -1.4 146,698 + -1.2 146,699 - -1.4 146,699 - -0.6 146,699 - -1.2 146,699 - -2.5 147,031 + GFF5278 0.53 -1.1 147,032 - GFF5278 0.53 +0.1 147,077 - GFF5278 0.60 +0.4 147,077 - GFF5278 0.60 -2.0 147,088 + GFF5278 0.61 -1.7 147,217 + GFF5278 0.80 -0.7 147,217 + GFF5278 0.80 -0.8 147,218 - GFF5278 0.81 -1.0 147,218 - GFF5278 0.81 -0.5 147,218 - GFF5278 0.81 -0.4 147,218 - GFF5278 0.81 +0.8 147,661 + GFF5279 0.29 -1.6 147,662 - GFF5279 0.29 -3.5 147,670 + GFF5279 0.30 -1.9 147,670 + GFF5279 0.30 -1.1 147,670 + GFF5279 0.30 -1.8 147,670 + GFF5279 0.30 -1.4 147,670 + GFF5279 0.30 -0.5 147,671 - GFF5279 0.30 -1.5 147,671 - GFF5279 0.30 -0.2 147,671 - GFF5279 0.30 -2.1 147,671 - GFF5279 0.30 -1.2 147,671 - GFF5279 0.30 +0.6 147,880 + GFF5279 0.52 -2.1 148,074 + GFF5279 0.72 +0.8 148,074 + GFF5279 0.72 -1.5 148,075 - GFF5279 0.72 -1.0 148,075 - GFF5279 0.72 +2.8 148,323 + +0.1 148,324 - +0.3 148,324 - -0.9 148,447 - -1.2 148,533 + GFF5280 0.18 -1.0 148,533 + GFF5280 0.18 -1.0 148,533 + GFF5280 0.18 -0.3 148,533 + GFF5280 0.18 -1.5 148,534 - GFF5280 0.18 -0.2 148,534 - GFF5280 0.18 -0.5 148,534 - GFF5280 0.18 -0.9 149,175 + GFF5280 0.85 -1.7 149,175 + GFF5280 0.85 -1.4 149,175 + GFF5280 0.85 -0.0 149,175 + GFF5280 0.85 -0.2 149,175 + GFF5280 0.85 -1.7 149,175 + GFF5280 0.85 -0.5 149,176 - GFF5280 0.85 +0.5 149,176 - GFF5280 0.85 +0.2 149,176 - GFF5280 0.85 -0.2 149,176 - GFF5280 0.85 -0.7 149,193 + GFF5280 0.87 -0.9 149,313 + +0.2 149,313 + +0.6 149,313 + -0.1 149,313 + -0.3 149,313 + -1.1 149,313 + -0.6 149,314 - -0.4 149,314 - -1.9 149,314 - -0.7 149,317 + -0.7 149,317 + +0.1 149,317 + +0.5 149,317 + -0.2 149,317 + +0.2 149,317 + -0.2 149,318 - +0.6 149,318 - +1.0 149,318 - -0.5 149,318 - -2.0 149,318 - -0.2 149,318 - +1.0 149,345 + +0.5 149,345 + +0.2 149,346 - -0.3 149,346 - -0.4 149,346 - -0.7 149,346 - -0.9
Or see this region's nucleotide sequence